Thermodynamic aspects of the self-assembly of DsrA, a small noncoding RNA from Escherichia coli

• Authors: Frédéric Geinguenaud , Maeva Gesson , Véronique Arluison
• Languages of publication: EN

Abstracts

EN

DsrA is an Escherichia coli small noncoding RNA that acts by base pairing to some mRNAs in order to control their translation and turnover. It was recently shown that DsrA is able to self-associate in a way similar to DNA and to build nanostructures. Although functional consequence of this RNA self-assembly in vivo is not yet understood, the formation of such an assemblage more than likely influences the noncoding RNA function. We report here for the first time the thermodynamic basis of this natural RNA self-assembly. In particular we show that assembling of the ribonucleic acid is enthalpy driven and that the versatility of the RNA molecule is important for the polymerisation; indeed, an equivalent DNA sequence is unable to make a nanoassembly. The origin of the difference is discussed herein.

Keywords

EN

DsrA; small noncoding RNA; nucleic acid self-assembly; RNA nanotechnology

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2014
• Volume: 61
• Issue: 1
• Pages: 179-184

Dates

published

2014

received

2013-12-30

revised

2014-01-18

accepted

2014-01-20

(unknown)

2014-01-22

Contributors

author

Frédéric Geinguenaud

• UFR SMBH, Université Paris 13, Sorbonne Paris Cité, 93017 Bobigny, France

author

Maeva Gesson

• Laboratoire Léon Brillouin, Commissariat à l'Energie Atomique, CNRS-UMR 12, CEA-Saclay, Gif-sur-Yvette Cedex, 91191 France

author

Véronique Arluison

• Laboratoire Léon Brillouin, Commissariat à l'Energie Atomique, CNRS-UMR 12, CEA-Saclay, Gif-sur-Yvette Cedex, 91191 France

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