PL EN


Preferences help
enabled [disable] Abstract
Number of results
2014 | 61 | 1 | 163-169
Article title

Vaccinium myrtillus leaves and Frangula alnus bark derived extracts as potential antistaphylococcal agents

Content
Title variants
Languages of publication
EN
Abstracts
EN
Due to constantly increasing antibiotic resistance of pathogens and participation of the biofilms they make in various types of infections, a development of alternative therapeutic strategies becomes an urgent need. Taking advantage of the biological activity of plant-derived compounds can solve this problem. In this study antimicrobial, including those synergistic with classic antibiotics, and cytotoxic properties of newly-obtained extracts from Vaccinium myrtillus leaves (VLE) and Frangula alnus bark (FBE) were evaluated. Both tested extracts exhibited relevant antistaphylococcal activity (MIC range 0.75-1.5 mg/mL) accompanied by a relativly low cytotoxic effect on mammalian cells (BI > 1). Phytochemical analysis of the extracts tested showed a high total content of phenolic compounds with the predominance of hydroxycinnamic acids in VLE and hydroxybenzoic acids and flavanols in FBE. Widely described in the literature antimicrobial properties of phenolics were probably connected with the biological activity of the extracts tested. We also report that the presence of VLE or FBE at sub-MIC concentrations enhances biocidal potential of vancomycin and linezolid. Therefore, we are considering a possibility of an alternative therapy for local infections caused by S. aureus by combining classic antibiotics with plant-derived extracts.
Publisher

Year
Volume
61
Issue
1
Pages
163-169
Physical description
Dates
published
2014
received
2013-10-31
revised
2014-03-11
accepted
2014-03-17
(unknown)
2014-03-20
Contributors
  • Department of Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Department of Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
  • Department of Biotechnology and Food Sciences, Institute of Technical Biochemistry, Lodz University of Technology, Łódź, Poland
  • Department of Biotechnology and Food Sciences, Institute of Technical Biochemistry, Lodz University of Technology, Łódź, Poland
  • Department of Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Poland
References
  • Alviano DS, Alviano CS (2009) Plant extracts: search for new alternatives to treat microbial diseases. Curr Pharm Biotechnol 10: 106-21.
  • Al-Zahrani SHM (2012) Antibacterial activities of gallic acid and gallic acid methyl ester on methicillin-resistant Staphylococcus aureus. J Am Sci 8: 7-12.
  • Appeldoorn CC, Bonnefoy A, Lutters BC, Daenens K, van Berkel TJ, Hoylaerts MF, Biessen EA (2005) Gallic acid antagonizes P-selectin-mediated platelet-leukocyte interactions. Implications for the French Paradox. Circulation 111: 106-112.
  • Aqil F, Khan MS, Owais M, Ahmad I (2005) Effect of certain bioactive plant extracts on clinical isolates of ß-lactamase producing methicillin resistant Staphylococcus aureus. J Basic Microbiol 45: 106-14.
  • Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils - a review. Food Chem Toxicol 46: 446-475.
  • Budzyńska A, Więckowska-Szakiel M, Sadowska B, Kalemba D, Różalska B (2011) Antibiofilm activity of selected plant essential oils and their major components. Polish J Microbiol 60: 35-41.
  • Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A (2011) Antibacterial activity of thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement Altern Med 11: 1-6. Available from: http://www.biomedcentral.com/1472-6882/11/29.
  • Clinical and Laboratory Standards Institute (2009) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M07-A8 8th edn, Vol 29, No 2, pp 5-30. Wayne, USA.
  • Cushnie TP, Lamb AJ (2005) Antimicrobial activity of flavonoids. Int J Antimicrob Agents 26: 343-356.
  • Cushnie TP, Lamb AJ (2011) Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 38: 99-107.
  • Dyrby M, Westergaard N, Stapelfeldt H (2001) Light and heat sensitivity of red cabbage extract in soft drink model system. Food Chem 72: 431-437.
  • Fankam AG, Kuete V, Voukeng IK, Kuiate JR, Pages JM (2011) Antibacterial activities of selected Cameroonian spices and their synergistic effects with antibiotics against multidrug-resistant phenotypes. BMC Compl Altern Med 11: 104-114.
  • Fraga CG, Galleano M, Verstraeten SV, Oteiza PI (2010) Basic biochemical mechanisms behind the health benefits of polyphenols. Mol Aspects Med 31: 435-45.
  • Gauwerky K, Borelli C, Korting HC (2009) Targeting virulence: A new paradigm for antifungals. Drug Discov Today 14: 214-222.
  • Gholamhoseinian N, Mansouri S, Rahighi S (2009) Effects of sub-inhibitory concentration of Myrtus communis leave extracts on the induction of free radicals in Staphylococcus aureus: a possible mechanism for the antibacterial action. Asian J Plant Sci 8: 551-556.
  • Gibbons S (2008) Phytochemicals for bacterial resistance-strengths, weaknesses and opportunities. Planta Medica 74: 594-602.
  • Hartzfeld PW, Forkner R, Hunter MD, Hagerman AE (2002) Determination of hydrolyzable tannins (gallotannins and ellagitannins) after reaction with potassium iodate. J Agric Food Chem 50: 1785-1790.
  • Hokkanen J, Mattila S, Jaakola L, Pirttila AM, Tolonen A (2009) Identification of phenolic compounds from lingonberry (Vaccinium vitis-idaea L.), bilberry (Vaccinium myrtillus L.) and hybrid bilberry (Vaccinium intermedium Ruthe L.) leaves. J Agric Food Chem 57: 9437-9447.
  • Kalil AC, Klompas M, Haynatzki G, Rupp ME (2013) Treatment of hospital-acquired pneumonia with linezolid or vancomycin: a systematic review and meta-analysis. BMJ Open 3: doi: 10.1136/bmjopen-2013-003912.
  • Kharlamova T, Esposito F, Zinzula L, Floris G, Cheng YC, Dutschman G, Tramontano E (2009) Inhibition of HIV-1 ribonuclease H activity by novel frangula-emodine derivatives. Med Chem 5: 398-410.
  • Kurlenda J, Grinholc M (2012) Alternative therapies in Staphylococcus aureus diseases. Acta Biochim Polon 59: 171-184.
  • Kuźma Ł, Wysokińska H, Różalski M et al. (2012) Antimicrobial and anti-biofilm properties of new taxidione derivative from hairy roots of Salvia austriaca. Phytomedicine 19: 1285-1287.
  • Manojlovic NT, Solujic S, Sukdolak S, Milosev M (2005) Antifungal activity of Rubia tinctorum, Rhamnus frangula and Caloplaca cerina. Fitoterapia 76: 244-246.
  • Martos J, Luque CM, González-Rodriguez MP, Arias-Moliz MT, Baca P (2013) Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide against Enterococcus faecalis biofilm. Eur J Microbiol Immunol (Bp) 3: 44-48.
  • McMahon MA, Blair IS, Moore JE, McDowell DA (2007) Habituation to sub-lethal concentrations of tee tree oil (Melaleuca alternifolia) is associated with reduced susceptibility to antibiotics in human pathogens. J Antimicrob Chemother 59: 125-127.
  • Müller G, Kramer A (2008) Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity. J Antimicrob Chemother 61: 1281-1287.
  • Nguyen HM, Graber CJ (2010) Limitations of antibiotic options for invasive infections caused by methicillin-resistant Staphylococcus aureus is combination therapy the answer? J Antimicrob Chemother 65: 24-36.
  • Palombo EA (2011) Traditional medicinal plant extracts and natural products with activity against oral bacteria: Potential application in the prevention and treatment of oral diseases. Evid Based Complement Alternat Med 2011: article ID 680354, 15 pages.
  • Rafii F, Shahverdi AR (2007) Comparison of essential oils from three plants for enhancement of antimicrobial activity of nitrofurantoin against enterobacteria. Chemotherapy 53: 21-25.
  • Rösch D, Bergmann M, Knorr D, Kroh LW (2003) Structure-antioxidant efficiency relationships of phenolic compounds and their contribution to the antioxidant activity of sea buckthorn juice. J Agric Food Chem 51: 4233-4239.
  • Różalski M, Micota B, Sadowska B, Stochmal A, Jędrejek D, Więckowska-Szakiel M, Różalska B (2013) Antiadherent and antibiofilm activity of Humulus lupulus L. derived products: new pharmacological properties. BioMed Res Intern 7 pages [DOI: 10.1155/2013/101089].
  • Swain T, Hillis WE (1959) The phenolics constituents of Prunus domestica II. J Sci Food Agric 10: 135-138.
  • Tegos G, Stermitz FR, Lomovskaya O, Lewis K (2002) Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrob Agents Chemother 46: 3133-3141.
  • Trumbeckaite S, Burdulis D, Raudone L, Liobikas J, Toleikis A, Janulis V (2013) Direct effects of Vaccinium myrtillus L. fruit extracts on rat heart mitochondrial functions. Phytother Res 27: 499-506.
  • Walencka E, Różalska S, Wysokińska H, Różalski M, Kuźma Ł, Różalska B (2007) Salvipisone and aethiopinone from Salvia sclarea hairy roots modulate staphylococcal antibiotic resistance and express anti-biofilm activity. Planta Med 73: 545-551.
  • Yamaura K, Shimada M, Ueno K (2011) Anthocyanins from bilberry (Vaccinium myrtillus L.) alleviate pruritus in a mouse model of chronic allergic contact dermatitis. Pharmacognosy Res 3: 173-177.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv61p163kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.