Preferences help
enabled [disable] Abstract
Number of results
2013 | 60 | 4 | 871-873
Article title

Prp4 kinase is required for proper segregation of chromosomes during meiosis in Schizosaccharomyces pombe

Title variants
Languages of publication
Chromosome segregation during meiosis is a complex process, which leads to production of four haploid gametes from two precursor cells. Reversible phosphorylation of proteins plays a crucial role in this process. The Schizosaccharomyces pombe Prp4 is an essential serine/threonine protein kinase, which belongs to the Clk/Sty family. To study the role of Prp4 in meiosis, we analysed chromosome segregation in a strain carrying conditional analog-sensitive allele of Prp4 protein kinase (prp4-as2). Our data show, that Prp4 protein kinase plays important role in chromosome segregation during meiosis, as revealed by enhanced missegregation of chromosomes in prp4-as2 mutant cells.
Physical description
  • Slovak University of Agriculture, Department of Genetics and Breeding Biology, Nitra, Slovakia
  • Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia
  • Slovak University of Agriculture, Department of Genetics and Breeding Biology, Nitra, Slovakia
  • Cipak L, Zhang C, Kovacikova I, Rumpf C, Miadokova E, Shokat KM, Gregan J (2011) Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe. Cell Cycle 10: 3527-3532.
  • Gregan J, Zhang C, Rumpf C, Cipak L, Li Z, Uluocak P, Nasmyth K, Shokat KM (2007) Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe. Nat Protoc 2: 2996-3000.
  • Gross T, Lützelberger M, Weigmann H, Klingenhoff A, Shenoy S, Käufer NF (1997) Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue. Nucleic Acids Res 25: 1028-1035.
  • Gutierrez-Caballero C, Cebollero LR, Pendas AM (2012) Shugoshins: from protectors of cohesion to versatile adaptors at the centromere. Trends Genet 28: 351-360.
  • Kovacikova I, Polakova S, Benko Z, Cipak L, Zhang L, Rumpf C, Miadokova E, Gregan J (2013) A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe. Cell Cycle 12: 618-624.
  • Krebs EG (1993) Nobel Lecture. Protein phosphorylation and cellular regulation I. Biosci Rep 13: 127-142.
  • Nousiainen M, Sillje HH, Sauer G, Nigg EA, Korner R (2006) Phosphoproteome analysis of the human mitotic spindle. Proc Natl Acad Sci USA 103: 5391-5396.
  • Montembault E, Dutertre S, Prigent C, Giet R (2007) PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. J Cell Biol 179: 601-609.
  • Rabitsch KP, Gregan J, Schleiffer A, Javerzat JP, Eisenhaber F, Nasmyth K (2004) Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II. Curr Biol 14: 287-301.
  • Rosenberg GH, Alahari SK, Kaufer NF (1991) Prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns. Mol Gen Genet 226: 305-309.
  • Rumpf C, Cipak L, Dudas A, Benko Z, Pozgajova M, Riedel CG, Ammerer G, Mechtler K, Gregan J (2010) Casein kinase 1 is required for efficient removal of Rec8 during meiosis I. Cell Cycle 9: 2657-2662.
  • Schneider M, Hsiao H, Will CL, Giet R, Urlaub H, Lührmann R (2010) Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation. Nat Struct Mol Biol 17: 216-221.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.