Bioavailability of hydrocarbons to bacterial consortia during Triton X-100 mediated biodegradation in aqueous media

• Authors: Daria Pęziak , Aleksandra Piotrowska , Roman Marecik , Piotr Lisiecki , Marta Woźniak , Alicja Szulc , Łukasz Ławniczak , Łukasz Chrzanowski
• Languages of publication: EN

Abstracts

EN

The aim of our study was to investigate the effect of Triton X-100 on the biodegradation efficiency of hexadecane and phenanthrene carried out by two bacterial consortia. It was established that the tested consortia were not able to directly uptake compounds closed in micelles. It was observed that in micellar systems the nonionic synthetic surfactant was preferentially degraded (the degradation efficiency of Triton X-100 after 21 days was 70% of the initial concentration - 500 mg/l), followed by a lesser decomposition of hydrocarbon released from the micelles (30% for hexadecane and 20% for phenanthrene). However, when hydrocarbons were used as the sole carbon source, 70% of hexadecane and 30% of phenanthrene were degraded. The degradation of the surfactant did not contribute to notable shifts in bacterial community dynamics, as determined by Real-Time PCR. The obtained results suggest that if surfactant-supplementation is to be used as an integral part of a bioremediation process, then possible bioavailability decrease due to entrapment of the contaminant into surfactant micelles should also be taken into consideration, as this phenomenon may have a negative impact on the biodegradation efficiency. Surfactant-induced mobilization of otherwise recalcitrant hydrocarbons may contribute to the spreading of contaminants in the environment and prevent their biodegradation.

Keywords

EN

biodegradation; bioavailability; hexadecane; phenanthrene; Triton X-100

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 4
• Pages: 789-793

Dates

published

2013

received

2013-10-15

revised

2013-12-04

accepted

2013-12-04

Contributors

author

Daria Pęziak

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

author

Aleksandra Piotrowska

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

author

Roman Marecik

• Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznań, Poland

author

Piotr Lisiecki

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

author

Marta Woźniak

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

author

Alicja Szulc

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

author

Łukasz Ławniczak

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

author

Łukasz Chrzanowski

• Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland

References

• Alias S, Hussain N-H, Omar M, Abdul-Talib S (2012) Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbon in contaminated sand by Sphingobacterium spiritovorum and Corynebaterium urealyticum. Int J Eng Phys Sci 6: 369-376.
• Broze G (1999) Handbook of Detergents Part A: Properties. Surfactant Science Series 82.
• Chrzanowski Ł, Owsianiak M, Szulc A, Marecik R, Piotrowska-Cyplik A, Olejnik-Schmidt AK, Staniewski J, Lisiecki P, Ciesielczyk F, Jesionowski T, Heipieper HJ (2011) Interactions between rhamnolipid biosurfactants and toxic chlorinated phenols enhance biodegradation of a model hydrocarbon-rich effluent. Int Biodeter Biodegr 65: 605-611.
• Chrzanowski Ł, Wick LY, Meulenkamp R, Kaestner M, Heipieper HJ (2009) Rhamnolipid biosurfactants decrease the toxicity of chlorinated phenols to Pseudomonas putida DOT-T1E. Lett Appl Microbiol 48: 756-762.
• Cyplik P, Schmidt M, Szulc A, Marecik R, Lisiecki P, Heipieper HJ, Owsianiak M, Vainshtein M, Chrzanowski Ł (2011) Relative quantitative PCR to assess bacterial community dynamics during biodegradation of diesel and biodiesel fuels under various aeration conditions. Biores Technol 102: 4347-4352.
• Dai Z, Wang Z, Xu JH, Qi H (2010) Assessing bioavailability of the solubilization of organic compound in nonionic surfactant micelles by dose-response analysis. Appl Microbiol Biotechnol 88: 327-339.
• Das N, Chandran P (2011) Microbial degradation of petroleum hydrocarbon contaminants. Biotechnol Res Int 2011: 1-13.
• Efroymson R, Alexander M (1991) Biodegradation by an Arthrobacter species of hydrocarbons partitioned into an organic solvent. Appl Environ Microbiol 57: 1441-1447.
• Graves D, Leavitt M (1991) Petroleum biodegradation in soil: The effect of direct application of surfactants. Remediation 1: 147-166.
• Guha S, Jaffe PR (1996) Biodegradation kinetics of phenantrene partitioned into the micellar phase of nonionic surfactants. Environ Sci Technol 30: 605-611.
• Guha S, Jaffe PR, (1996) Bioavailable of hydrophobic compounds partitioned into the micellar phase of nonionic surfactants. Environ Sci Technol 30: 1382-1391.
• Ławniczak Ł, Marecik R, Chrzanowski Ł, (2013) Contributions of biosurfactants to natural or induced bioremediation. Appl Microbiol Biotechnol 97: 2327-2339.
• Makkar RS, Rockne KJ (2003) Comparison of synthetic surfactants and biosurfactants in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environ Toxicol Chem 22: 2280-2292.
• Owsianiak M, Szulc A, Chrzanowski Ł, Cyplik P, Bogacki M, Olejnik-Schmidt AK, Heipieper HJ (2009a) Biodegradation and surfactant-mediated biodegradation of diesel fuel by 218 microbacterial consortia and not correlated to cell surface hydrophobicity. Appl Microbiol Biotechnol 84: 545-553.
• Owsianiak M, Chrzanowski L, Szulc A, Staniewski J, Olszanowski A, Olejnik-Schmidt AK, Heipieper HJ (2009b) Biodegradation of diesel/biodiesel blends by a consortium of hydrocarbon degraders: Effect of the type of blend and the addition of biosurfactants. Bioresource Technol 100: 1497-1500.
• Paria S (2008) Surfactant-enhanced remediation of organic contaminanted soil and water. Adv Colloid Interface Sci 138: 24-58.
• Stelmack PL, Gray MR, Pickard MA (1999) Bacterial adhesion to soil contaminants in the presence of surfactants. Appl Environ Microbiol 65: 163-168.
• Szymanski A, Lukaszewski Z (1996) Determination of poly(ethylene glycols) in environmental samples by the indirect tensammetric method. Analyst 121: 1897-1901.
• Wang Z (2011) Bioavailability of organic compounds solubilized in nonionic surfactant micelles. Appl Microbiol Biotechnol 89: 523-534.
• Willumsen A, Karlson U, Pritchard PH (1998) Response of fluoranthene-degrading bacteria to surfactants. Appl Microbiol Biotechnol 50: 475-483.
• Wyrwas B, Chrzanowski Ł, Ławniczak Ł, Szulc A, Cyplik P, Białas W, Szymański A, Hołderna-Odachowska A (2011) Utilization of Triton X-100 and polyethylene glycols during surfactant-mediated biodegradation of diesel fuel. J Hazard Mater 197: 97-103.
• Volkering F, Breure AM, Andel JG, Rulkens WH (1995) Influence of nonionic surfactants on bioavailability and biodegradation of polycyclic aromatic hydrocarbons. Appl Environ Microbiol 61: 1699-705.
• Yang J, Chen JJ, Lu Y (2009) Removal mechanism of lighf non-aqueus phase liquid from soil and groundwater by surfactants. China Environ Sci 30: 2153-2159.
• Zeng G, Fu H, Zhong H (2007) Co-degradation with glucose of four surfactants, CTAB, Trition X-100, SDS and Rhamnolipid, in liquid culture media and compost matrix. Biodegradation 18: 303-310.