Genetic polymorphism of Hucul horse population based on 17 microsatellite loci

• Authors: Agnieszka Fornal , Anna Radko , Agata Piestrzyńska-Kajtoch
• Languages of publication: EN

Abstracts

EN

Short tandem repeat (STR) loci, i.e. microsatellites are a class of genetic markers commonly used for population studies and parentage control. This study determined the usefulness of microsatellite markers recommended by International Society for Animal Genetics (ISAG) for identification and pedigree analysis in horses based on the example of Polish Hucul horse population (Equus caballus). The set of seventeen microsatellites loci was tested (AHT4, AHT5, ASB2, HMS2, HMS3, HMS6, HMS7, HTG10, HTG4, HTG6, HTG7, VHL20, ASB17, ASB23, CA425, HMS1, LEX3) for 216 individuals. All samples were genotyped and mean number of alleles per locus was estimated (7.00). Means of observed (Ho) and expected (He) heterozygosity were calculated 0.7288 and 0.7027, respectively. The observed heterozygosity was similar to the results of research on Hucul horse population in another area of Carpathians Mountains. The average polymorphism information content (PIC) for analyses of seventeen microsatellite markers indicates the usefulness of this set of markers for Hucul horse parentage testing.

Keywords

EN

microsatellite markers; STR; parentage testing; horse; Hucul horse; indigenous breeds

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 4
• Pages: 761-765

Dates

published

2013

received

2013-10-13

revised

2013-12-03

accepted

2013-12-12

Contributors

author

Agnieszka Fornal

• National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland

author

Anna Radko

• National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland

author

Agata Piestrzyńska-Kajtoch

• National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland

References

• Bordzoł A, Jackowski M (2008) Struktura genealogiczna populacji koni huculskich w Bieszczadzkim Parku Narodowym. Roczniki bieszczadzkie 16: 389-408 (in Polish).
• Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32: 314-331.
• DAD-IS (Domestic Animal Diversity Information System hosted by FAO) (2013.09.12).
• Dimsoski P (2003) Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses. Croatian Medical Journal 44: 332-335.
• Equine Genetics and Parentage Analysis Workshop (2012) Equine Genetics & Thoroghbred Parentage Testing Standardisation Workshop.
• Georgescu SE, Costache M (2012) Genetic characterization of romanian local breeds using microsatellite markers. In Analysis of Genetic Variation in Animals. Chapter 2: 27-44.
• Georgescu SE, Manea MA, Costache M (2008) The genetic structure of indigenous Romanian Hucul horse breed inferred from microsatellite data. Roumanian Biotechnological Letters 13: 4030-4036.
• Georgescu SE, Manea MA, Dudu A, Costache M (2011) Phylogenetic relationships of the Hucul horse from Romania inferred from mitochondrial D-loop variation. Genet Mol Res 10: 4104-4113.
• Huston KA (1998) Statistical analysis of STR data. Profiles in DNA 3: 14-15.
• Instruction Manual, Equine Genotypes™ Panel 1.1 (2011)
• Józsa C, Bán B, Mihók S, Bodó I (2005) DNA microsatellite test of Hutsul horses in Hungary. EAAP - 56th Annual Meeting.
• Kusza S, Priskin K, Ivankovic A, Jedrzejewska B, Podgorski T, Jávor A, Mihók S (2013) Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data. Biol J Linn Soc Lond 109: 54-65.
• Nei M (1987) Molecular Evolutionary Genetics. Columbia University Press.
• Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6: 288-295.
• Raymond M, Rousset F (1995) GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism. J Heredity 86: 248-249.
• Tautz D (1989) Hypervariability of simple sequences as general source for polymorphic DNA markers. Nucleic Acids Res 17: 6463-6471.
• Tomczyk-Wrona I (2009) Program ochrony zasobów genetycznych koni huculskich. 2013.09.12
• Trandžík J, Žídek R, Jakabová D, Buleca J, Massányi P, Haško M, Kozlík P (2006) Genetic diversity of Hucul horse, based on microsatellite data in Slovak Republic.
• Van de Goor LH, Panneman H, van Haeringen WA (2010) A proposal for standardization in forensic equine DNA typing: allele nomenclature for 17 equine-specific STR loci. Animal Genetics 41: 122-127.
• Ząbek T, Fornal A (2009) Evaluation of the 17-plex STR kit for parentage testing of polish Coldblood and Hucul horses. Ann Animal Sci 9: 363-372.
• Žídek R, Jakabová D, Trandzz̆ík J, Gralak B, Burócziová M, Buleca JJr, Maróti-Agóts A, Massányi P, Dvorz̆ák J, Rz̆iha J, Zöldág L (2009) Diversity analysis of Hucul horse population based on molecular genetic data. Magyar Állatorvosok Lapja 131: 685-691.