Preferences help
enabled [disable] Abstract
Number of results
2013 | 60 | 1 | 71-75
Article title

Genetic variants of uncoupling proteins-2 and -3 in relation to maximal oxygen uptake in different sports

Title variants
Languages of publication
Uncoupling proteins 2 and 3 (UCP2 and UCP3) as mitochondrial electron transporters are involved in regulation of ATP production and energy dissipation as heat. Energy efficiency plays an important role in physical performance, especially in aerobic fitness. The aim of this study was to examine the association between maximal oxygen uptake and genetic variants of the UCP2 and UCP3 genes. The studies were carried out in a group of 154 men and 85 women, professional athletes representing various sports and fitness levels and students of the University of Physical Education in Poznań. Physiological and molecular procedures were used, i.e. direct measurement of maximum oxygen uptake (VO2max) and analysis of an insertion/deletion (I/D) polymorphism in the 3'untranslated region of exon 8 of the UCP2 gene and a C>T substitution in exon 5 (Y210Y) of the UCP3 gene. No statistically significant associations were found, only certain trends. Insertion allele (I) of the I/D UCP2 and the T allele of the UCP3 gene were favourable in obtaining higher VO2max level and might be considered as endurance-related alleles.

Physical description
  • Department of Physiology, Faculty of Physical Education, Sport and Rehabilitation, The Eugeniusz Piasecki University School of Physical Education, Poznań, Poland
  • Department of Physiology, Faculty of Physical Education, Sport and Rehabilitation, The Eugeniusz Piasecki University School of Physical Education, Poznań, Poland
  • Department of Physiology, Faculty of Physical Education, Sport and Rehabilitation, The Eugeniusz Piasecki University School of Physical Education, Poznań, Poland
  • Computer Laboratory of the Faculty of Animal Breeding and Biology, Poznań, University of Life Sciences, Poznań, Poland
  • Ahmetov II, Williams AG, Popov DV et al (2009) The combined impact of metabolic gene polymorphism on elite endurance athletes status and related phenotypes. Hum Genet 126: 751-761.
  • Argyropoulos G, Brown AM, Willi SM, Zhu J, He Y, Reitman M et al (1998) Effects of mutations in the human uncoupling protein 3 gene on the respiratory quotient and fat oxidation in severe obesity and type 2 diabetes. J Clin Invest 102: 1345-1351.
  • Bellotti P, Benzi G, Dal Monte A, Donati A, Matteucci E et al (1978) Classificazione degli Sport e determinazione del mezzi di allenamento. Atleticastudi 3/4.
  • Beumann B Schierning B, Toubro S, Bibby BM, Sorensen T, Dalgaard L et al, (2001) The association between the val/ala-55 polymorphism of the uncoupling protein 2 gene and exercise efficiency. Int J Obes Relat Metab Disord 4: 467-471.
  • Boss O, Hagen T, Lowell BB (2000) Uncoupling proteins 2 and 3: Potential regulators of mitochondrial energy metabolism. Diabetes 49: 143-156.
  • Bouchard C, Perusse L, Chagnon YC, Warden C, Ricquier D et al (1997) Linkage between markers in the vicinity of the uncoupling protein 2 gene and resting metabolic rate in humans. Hum Molec Genet 6: 1887-1889.
  • Bray MS, Hagberg JM, Pérusse L, Rankinen T, Roth SM, Wolfarth B, Bouchard C. (2009) The human gene map for performance and health-related fitness phenotypes: the 2006-2007 update. Med Sci Sports Exerc 41: 35-73.
  • Crocco P, Montesanto A, Passarino G, Rose G (2011) A common polymorphism in the UCP3 promoter influences hand grip strength in elderly people. Biogerontology 12: 265-71.
  • Damcott CM, Feingold E, Moffet SP, Barmada MM, Marshall JA, Hamman RF et al (2004) Genetic variation in uncoupling protein 3 is associated with dietary intake and body composition in females. Metabolism 53: 458-464.
  • Erlanson-Albertsson C (2003) The role of uncoupling proteins in the regulation of metabolism. Acta Physiol Scand 178: 405-412.
  • Esterbauer H, Schneitler C, Oberkofler H, Ebenbichler C, Paulweber B, Sandhofer F et al (2001) A common polymorphism in the promoter of UCP2 is associated with decreased risk of obesity in middle-aged humans. Nature Genet 28: 178-183.
  • Esteves TC, Brand MD (2005) The reactions catalysed by the mitochondrial uncoupling proteins UCP2 and UCP3. Biochim Biophys Acta 1709: 35-44.
  • Fleury C, Sanchis D (1999) The mitochondrial uncoupling protein-2: current status. Int J Biochem Cell Biol 31: 1261-1278.
  • Gronek P, Holdys J, Konarski J, Kryściak J, Wolc A (2013) ACE genotype in professional field hockey players. Trends Sport Sci 1: 38-42.
  • Hesselink MKC, Greenhaff PL, Constantin-Teodosiu D, Hultman E, Saris WHM, Nieuwlaat R et al (2003) Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo. J Clin Invest 111: 479-486.
  • Hoeks J, Hesselink MK, Schrauwen P (2006) Involvement of UCP3 in mild uncoupling and lipotoxicity. Exp Gerontol 41: 658-662.
  • Ježek P (1999) Fatty acid interaction with mitochondrial uncoupling proteins. J Bioenerg Biomembr 31: 457-466.
  • Ježek P (2002) Possibile physiological role of mitochondria uncoupling proteins - UCPn. Int J Biochem Cell Biol 34: 1190-1206.
  • Kimm SY, Glynn NW, Aston CE, Damcott CM, Poehlman ET, Daniels SR et al (2002) Racial differences in the relation between uncoupling protein genes and resting energy expenditure. Am J Clin Nutr 75: 714-719.
  • Krauss S, Zhang CY, Lowell BB (2005) The mitochondrial uncoupling-protein homologues. Nat Rev Mol Cell Biol 6: 248-261.
  • Lanouette Ch-M, Chagnon YC, Rice T, Pérusse L, Muzzin P, Giacobino J-P et al (2002) Uncoupling protein 3 gene is associated with body composition changes with training in HERITAGE study. J Appl Physiol 92: 1111-1118.
  • Lippi G, Longo UG, Maffulli N (2010) Genetics and sports. Br Med Bull 93: 27-47.
  • Maciejewska-Karłowska A (2013) Polymorphisms in the Peroxisome Proliferator-Activated Receptor genes: relevance for athletic performance Trends Sport Sci 1: 5-15.
  • Matsunaga T, Gu N, Yamazaki H, Tsuda M, Adachi T, Yasuda K, Moritani T et al (2009) Association of UCP2 and UCP3 polymorphisms with heart rate variability in Japanese men. J Hypertens 27: 305-313.
  • Nabben M, Hoeks J (2008) Mitochondrial uncoupling protein 3 and its role in cardiac- and skeletal muscle metabolism. Physiol Behav 94: 259-269.
  • Nabben M, Shabalina IG, Moonen-Kornips E, van Beurden D, Cannon B, Schrauwen P, Nedergaard J, Hoeks J (2011) Uncoupled respiration, ROS production, acute lipotoxicity and oxidative damage in isolated skeletal muscle mitochondria from UCP3-ablated mice. Biochim Biophys Acta 1807: 1095-1105.
  • Otabe S, Clement K, Dina C, Pelloux V, Guy-Grand B, Froguel P et al. (2000) A genetic variation in the 5' flanking region of the UCP3 gene is associated with body mass index in humans in interaction with physical activity. Diabetologia 43: 245-249.
  • Rose G, Crocco P, De Rango F, Montesanto A, Passarino G (2011) Further support to the uncoupling-to-survive theory: the genetic variation of human UCP genes is associated with longevity. PLoS One 6: e29650. doi: 10.1371/journal.pone.0029650.
  • Rosmond R, Bouchard C, Björntorp P (2002) Lack of association between the uncoupling protein-2 Ala55Val gene polymorphism and phenotypic features of the Metabolic Syndrome. Biochim Biophys Acta 1588: 103-105.
  • Russell A, Wadley G, Snow R, Giacobino JP, Muzzin P, Garnham A et al. (2002) Slow component of VO2 kinetics: the effect of training status, fibre type, UCP3 mRNA and citrate synthase activity. Int J Obes Relat Metab Disord 26: 157-164.
  • Sawczuk M, Maciejewska-Karłowska A, Cięszczyk P (2013) A single nucleotide polymorphism rs553668 in ADRA2A gene and Polish elite endurance athlete status. Trends Sport Sci 1: 32-37.
  • Schrauwen P, Hoeks J, Hesselink MK (2006) Putative function and physiological relevance of the mitochondrial uncoupling protein-3: involvement in fatty acid metabolism? Prog Lipid Res 45: 17-41.
  • Schrauwen P, Russell AP, Moonen-Kornips E et al. (2005) Effect of 2 weeks of endurance training on uncoupling protein 3 content in untrained human subjects. Acta Physiol Scand 183: 273-280.
  • Stuart JA, Cadenas S, Jekabsons MB, Roussel D, Brand MD et al (2001) Mitochondrial proton leak and the uncoupling protein 1 homologues. Biochim Biophys Acta 1504: 144-158.
  • Tu N, Chen H, Winnikes U, Reinert I, Marmann G, Pirke KM, Lentes KU (1999) Structural organization and mutational analysis of the human uncoupling protein-2 (hUCP2) gene. Life Sci 64: PL41-50.
  • Urhammer SA, Dalgaard LT, Sørensen TI, Tybjaerg-Hansen A, Echwald SM, Andersen T et al (1998) Organisation of the coding exons and mutational screening of the uncoupling protein 3 gene in subjects with juvenile-onset obesity. Diabetologia 41: 241-244.
  • Walder K, Norman RA, Hanson RL, Schrauwen P, Neverova M, Jenkinson CP et al (1998) Association between uncoupling protein polymorphisms (UCP2-UCP3) and energy metabolism/obesity in Pima Indians. Hum Mol Gen 7: 1431-1435.
  • Wallace DC (2005) The mitochondrial genome in human adaptive radiation and disease: on the road to therapeutics and performance enhancement. Gene 354: 169-180.
  • Warden C (1999) Genetics of uncoupling proteins in humans. Int J Obes Relat Metab Disord 23: S46-S48.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.