PL EN


Preferences help
enabled [disable] Abstract
Number of results
2013 | 60 | 4 | 701-706
Article title

Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H Nuclear Magnetic Resonance search for candidate biomarkers

Content
Title variants
Languages of publication
EN
Abstracts
EN
Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.
Publisher

Year
Volume
60
Issue
4
Pages
701-706
Physical description
Dates
published
2013
received
2013-10-31
revised
2013-11-25
accepted
2013-12-12
Contributors
author
  • Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland
  • Faculty of Chemistry, Opole University, Opole, Poland
  • Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland
  • Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wrocław, Poland
  • Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wrocław, Poland
  • Department of Microbiology, Medical University of Wroclaw, Wrocław, Poland
  • Faculty of Chemistry, Opole University, Opole, Poland
References
  • Bertini I, Cacciatore S, Jensen B, Schou J, Johansen J, Kruhoffer M, Luchinat C, Nielsen D, Turano P (2012) Metabolomic NMR Fingerprinting to Identify and Predict Survival of Patients with Metastatic Colorectal Cancer. Cancer Res 72: 356-364.
  • Bjarnsholt T, Kirketerp-Møller K, Jensen PØ, Madsen KG, Phipps R, Krogfelt K, Høiby N, Givskov M (2008) Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen 16: 2-10.
  • Bjarnsholt T (2011) Biofilm Infections. Springer Science, ISBN978-1-4419-6083-2.
  • Bothwell J, Griffin J (2010) An introduction to biological nuclear magnetic resonance spectroscopy. Biol Rev Camb Philos Soc 86: 493-510.
  • Declerck P (2010) Biofilms: the environmental playground of Legionella. Environ Microbiol 12: 557-566.
  • Fiehn O (2002) Metabolomics - the link between genotypes and phenotypes. Plant Mol Biol 48: 155-171.
  • Gupta A, Dwivedi M, Mahdi A, Khetrapal L, Bhandari M (2012) Broad identification of bacterial type in urinary tract infection using (1)h NMR spectroscopy. J Proteome Res 11: 1844-1854.
  • Gaupp R, Schlag S, Liebeke M, Lalk M, Gotz F (2010) Advantage of Upregulation of Succinate Dehydrogenase in Staphylococcus aureus Biofilms. J Bacteriol 192: 2385-2394.
  • Graham J, Wilkinson B (1992) Staphylococcus aureus osmoregulation: roles for choline, glycine betaine, proline, and taurine. J Bacteriol 174: 2711-2716.
  • Gross M, Cramton S, Gotz F, Peschel A (2001) Key role of teichoic acid net charge in Staphylococcus aureus colonization of artificial surfaces. Infect Immun 69: 3423-3426.
  • Hochbaum A, Kolodkin-Gal I, Foulston L, Kolter R, Aizenberg J, Losick R (2011) Inhibitory effects of d-amino acids on Staphylococcus aureus biofilm development. J Bacteriol 193: 5616-5623.
  • Jawien A et al. (2012) Guidelines for local and systemic treatment of infected chronic wounds. Leczenie Ran 9: 77-91. (in Polish and English).
  • Li X, Chen H, Jia W, Xie G (2013) A metabolomics-based strategy for the quality control of traditional Chinese medicine: shengmai infection as a case study. Evid Based Complemnet alternat Med doi: 10.1155/2013/836179.
  • Mahmoud Gad, Abdel Aziz, Ibrahem A (2012) In-vitro adhesion of Staphylococcus spp. to certain orthopaedic biomaterials and expression of adhesion genes. J App Pharm Scien 6: 145-149.
  • Montanaro L, Speziale P, Campoccia D, Ravaioli S, Cangini I, Pietrocola G, Giannini S, Arciola CR (2011) Scenery of Staphylococcus implant infections. Future Microbiol 6: 1329-1349.
  • Nicholson J, Lindon J, Holmes E (1999) 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data. Xenobiotica 29: 1181-1189.
  • Pohl K, Francois P, Stenz L, Schlin F, Geiger T, Herbert S, Goerke C, Schrenzel J, Wolz C (2009) CodY in Staphylococcus aureus: a regulatory link between metabolism and virulence gene expression. J Bacteriol 191: 2953-2963.
  • Resch A, Rosenstein R, Nerz C, Gotz F (2005) Differential gene expression profiling of Staphylococcus aureus cultivated under biofilm and planktonic conditions. Appl Environ Microbiol 71: 2663-2673.
  • Sadowska B, Wieckowska-Szakiel M, Paszkiewicz M, Rozalska B (2013) The immunomodulatory activity of Staphylococcus aureus products derived from biofilm and planktonic cultures. Arch Immunol Ther Exp 61: 413-420.
  • Schievano E, Stocchero M, Morelato E, Facchin C, Mammi S (2012) An-NMR-based metabolomic approach to identify the botanical origin of honey. Metabolomics 8: 679-690.
  • Schlag S, Nerz Ch, Birkenstock T, Altenberend F, Gotz F (2007) Inhibition of Staphylococcal biofilm formation by nitrite. J Bacteriol 189: 7911-7919.
  • Secor P, James G, Fleckman P, Olerud J, McInnerney K, Stewart P (2011) Staphylococcus aureus biofilm and planktonic cultures differentially impact gene expression, mapk phosphorylation, and cytokine production in human keratinocytes. BMC Microbiol 11: 143. doi: 10.1186/1471-2180-11-143.
  • Shunmuganperumal T et al. (2010) Biofilm Eradication and Prevention; John Wiley & Sons, ISBN 978-0-470-47996-4.
  • Widegrow A (2008) Persistence of the chronic wound - implicating biofilm. Wound Heal South Afr 1: 05-07.
  • Wolcott R, Rhoads D (2008) A study of biofilm-based wound management in subjects with crirical limb ischaemia. J Wound Care 17: 145-155.
  • Wolcott R, Dowd S, Kennedy J, Jones C (2010) Biofilm - based wound care. Adv in W Care 1: 311-318 .
  • Zhang B, Powers R (2012) Analysis of bacterial biofilms using NMR-based metabolomics. Future Med Chem 4: 1273-1306
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p701kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.