PL EN


Preferences help
enabled [disable] Abstract
Number of results
2013 | 60 | 4 | 695-700
Article title

Comparative study of metal induced phospholipid modifications in the heavy metal tolerant filamentous fungus Paecilomyces marquandii and implications for the fungal membrane integrity

Content
Title variants
Languages of publication
EN
Abstracts
EN
In this work we compared the effect of five heavy metals: Zn, Pb, Cd, Ni and Cu on phospholipid composition of the ubiquitous soil fungus Paecilomyces marquandii, originating from a strongly metal polluted area and characterized by high tolerance to these elements. Cd, Ni and Cu caused an increase in phosphatidylcholine (PC). Only Pb decreased PC content, which was accompanied by a significant rise in the phosphatidic acids (PA) level, probably due to activation of phospholipase D which hydrolyzes PC to PA. This could result in membrane fluidity disturbance, and thus affect its integrity. The assessment of propidium iodide influx showed strong disturbance of membrane integrity for Cu and Pb stressed mycelia, whereas mycelia treated with Ni were impermeable to this dye. The results obtained revealed a strong Cu and Pb toxicity involving disruption of membrane integrity. Pb action was reflected by lipid composition, whereas changes in Cu treated mycelia did not completely elucidate its harmful effect on the membrane, which was most probably caused by Cu induced lipid peroxidation. Zn did not induce quantitative changes in PC and phosphatidylethanolamine (PE) but caused changes in phospholipid lipid saturation, which appears to be important for fungus adaptation to the presence of metals. The enhanced PC content balanced by higher PC saturation can help in the maintenance of proper membrane fluidity and result in alleviating the Cd and Ni induced stress. These results will allow to clarify the mechanism of Pb toxicity and help to elucidate the cellular basis of fungal membrane adaptation to heavy metals.
Publisher

Year
Volume
60
Issue
4
Pages
695-700
Physical description
Dates
published
2013
received
2013-10-30
revised
2013-12-02
accepted
2013-12-03
Contributors
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
References
  • Avery SV, Howlett NG, Radice S (1996) Copper toxicity towards Saccharomyces cerevisiae: dependence on plasma membrane fatty acid composition. Appl Environ Microb 62: 3960-3966.
  • Azevedo MM, Carvalho A, Pascoal C, Rodrigues F, Cassio F (2007) Responses of antioxidant defenses to Cu and Zn stress in two aquatic fungi. Sci Total Environ 377: 233-243.
  • Belyaeva EA, Glazunov VV, Korotkov SM (2004) Cd2+-promoted mitochondrial permeability transition: a comparison with other heavy metals. Acta Biochim Pol 51: 545-551.
  • Bernat P, Gajewska E, Bernat T, Wielanek M (2013) Characterisation of the wheat phospholipid fraction in the presence of nickiel and/or selenium. Plant Growth Regul DOI 10.1007/s10725-013-9848-x.
  • Bou Khalil M, Hou W, Zhou H, Elisma F, Swayne LA, Blanchard AP, Yao Z, Bennett SAL, Figeys D (2010) Lipidomics era: accomplishments and challenges. Mass Spectrom Rev 29: 877-929.
  • Carman GM, Henry SA (2007) Phosphatidic acid plays a central role in the transcriptional regulation of glycerophospholipid synthesis in Saccharomyces cerevisiae. J Biol Chem 282: 37293-37297.
  • Čertík M, Breierová E, Juršíková P (2005) Effect of cadmium on lipid composition of Aureobasidium pullulans grown with added extracellular polysaccharides. Int Biodeterior Biodegradation 55: 195-202.
  • Chmielowska-Bąk J, Izbiańska K, Deckert J (2013) The toxic Doppelganger: on the ionic and molecular mimicry of cadmium. Acta Biochim Pol 60: 369-374.
  • de Kroon AI (2007) Metabolism of phosphatidylcholine and its implications for lipid acyl chain composition in Saccharomyces cerevisiae. Biochim Biophys Acta 1771: 343-352.
  • de Kroon AI, Rijken PJ, De Smet CH (2013) Checks and balances in phospholipid class and acyl chain homeostasis, the yeast perspective. Prog Lipid Res 52: 374-394.
  • Dercová K, Čertík M, Mal'ová A, Sejáková Z (2004) Effect of chlorophenols on the membrane lipids of bacterial cells. Int Biodeterior Biodegradation 54: 251-254.
  • Folch J, Lees M, Sloane-Stanley G (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 199: 833-841.
  • Gadd GM (2010) Metals, minerals and microbes: geomicrobiology and bioremediation. Microbiology 156: 609-643.
  • Garcia JJ, Martinez-Ballarin E, Millan-Plano S, Allue JL, Albendea C, Fuentes L, Escanero JF (2005) Effects of trace elements on membrane fluidity. J Trace Elem Med Biol 19: 19-22.
  • Howlett NG, Avery SV (1997) Relationship between cadmium sensitivity and degree of plasma membrane fatty acid unsaturation in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 48: 539-545.
  • Kaur A, Chaudhary A, Kaur A, Choudhary R, Kaushik R (2005) Phospholipid fatty acid - a bioindicator of environment monitoring and assessment in soil ecosystem. Curr Sci 89: 1103-1112.
  • Li Z, Agellon LB, Allen TM, Umeda M, Jewell L, Mason A, Vance DE (2006) The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell Metab 3: 321-331.
  • Lisowska K, Szemraj J, Różalska S, Długoński J (2006) The expression of cytochrome P-450 and cytochrome P-450 reductase genes in the simultaneous transformation of corticosteroids and phenanthrene by Cunninghamella elegans. FEMS Micrbiol Lett 261: 175-180.
  • Martins MN, Santos NA, Curti C, Bianchi ML, Santos AC (2008) Cisplatin induces mitochondrial oxidative stress with resultant energetic metabolism impairment, membrane rigidification and apoptosis in rat liver. J Appl Toxicol 28: 337-344.
  • Masia A, Avery SV, Zoroddu MA, Gadd GM (1998) Enrichment with a polyunsaturated fatty acid enhances the survival of Saccharomyces cerevisiae in the presence of tributyltin. FEMS Microbiol Lett 167: 321-326.
  • Niebergal LJ, Vance DE (2012) The ratio of phosphatidylcholine to phosphatidylethanolamine does not predict integrity of growing MT58 Chinese hamster ovary cells. Biochim Biophys Acta 1821: 324-334.
  • Orlean P, Menon AK (2007) Thematic review series: lipid posttranslational modifications. GPI anchors of protein in yeast and mammalian cells, or: how we learned to stop worrying and low glycophospholipids. J Lipid Res 48: 993-1011.
  • Paraszkiewicz K, Bernat P, Długoński J (2009) Effect of nickel, copper, and zinc on emulsifier production and saturation of cellular fatty acids in filamentous fungus Curvularia lunata. Int Biodeterior Biodegradation 63: 100-105.
  • Paraszkiewicz K, Bernat P, Naliwajski M, Długoński J (2010) Lipid peroxidation in the fungus Curvularia lunata exposed to nickel. Arch Microbiol 192: 135-141.
  • Paraszkiewicz K, Frycie A, Słaba M, Długoński J (2007) Enhancement of emulsifier production by Curvularia lunata in cadmium, zinc and lead presence. Biometals 20: 797-805.
  • Quartacci MF, Cosi E, Navari-Izzo F (2001) Lipids and NADPH-dependent superoxide production in plasma membrane vesicles from roots of wheat grown under copper deficiency or excess. J Exp Bot 52: 77-84.
  • Reynolds TB (2009) Strategies for acquiring the phospholipid metabolite inositol in pathogenic bacteria, fungi and protozoa: making and taking it. Microbiology 155: 1386-1396.
  • Šimočková M, Holič R, Tahotná D, Patton-Vogt J, Griač P (2008) Yeast Pgc1p (YPL206c) controls the amount of phosphatidylglycerol via a phospholipase C-type degradation mechanism. J Biol Chem 283: 17107-17115.
  • Słaba M, Bizukojć M, Pałecz B, Długoński J (2005) Kinetic study of the toxicity of zinc and lead ions to the heavy metals accumulating fungus Paecilomyces marquandii. Bioprocess Biosyst Eng 28: 185-197.
  • Słaba M, Długoński J (2011) Efficient Zn2+ and Pb2+ uptake by filamentous fungus Paecilomyces marquandii with engagement of metal hydrocarbonates precipitation. Int Biodeterior Biodegradation 65: 954-960.
  • Słaba M, Gajewska E, Bernat P, Fornalska M, Długoński J (2013) Adaptive alterations in the fatty acids composition under induced oxidative stress in heavy metal-tolerant filamentous fungus Paecilomyces marquandii cultured in ascorbic acid presence. Environ Sci Pollut Res Int 20: 3423-3434.
  • Stohs SJ, Bagchi D (1995) Oxidative mechanisms in the toxicity of metal ions. Free Radical Biol Med 18: 321-336.
  • Su K, Bremer DJ, Jeannotte R, Welti R, Yang C (2009) Membrane lipid composition and heat tolerance in cool-season turfgrasses, including a hybrid bluegrass. J Am Soc Hortic Sci 134: 511-520.
  • Vance JE, Steenbergen R (2005) Metabolism and functions of phosphatidylserine. Prog Lipid Res 44: 207-234.
  • Welti R, Li W, Li M, Sang Y, Biesiada H, Zhou HE, Rajashekar CB, Williams TD, Wang X (2002) Profiling membrane lipids in plant stress responses. Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis. J Biol Chem 277: 31994-32002.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p695kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.