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2013 | 60 | 4 | 677-682
Article title

Utilization of 4-n-nonylphenol by Metarhizium sp. isolates

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Nonylphenol (4-NP) is a xenobiotic classified as an endocrine disrupting compound with an ability to interfere with hormonal systems of numerous organisms including humans. It is widely distributed not only in aquatic but also in terrestrial systems. The aim of this study was to evaluate the ability of cosmopolitan fungus Metarhizium (commonly persistent in soil as a facultative insect pathogen, controlling populations of arthropods in natural environment) to degrade 4-n-nonylphenol. All isolates examined in this work were identified to a species rank based on five, independent genetic markers. Among eight Metarhizium strains; six of them have been identified as M. robertsii, and two others as M. brunneum and M. lepidiotae. All investigated Metarhzium isolates were found to eliminate 4-n-NP with significant efficiency (initial xenobiotic concentration 50 mg L-1). The degradation process was very effective and at 24h of incubation 50-90% of 4-n-NP was eliminated by certain strains, while extended incubation resulted in further utilization of this compound. At the end of the experiments 64-99% of 4-n-NP was removed from the culture medium. Additionally, in all tested cultures three major metabolites were detected: 4-hydroxybenzoic acid; 2-(4-hydroxyphenyl)acetic acid and 4-hydroxyphenylpentanoic acid. The obtained results indicate that Metarhizium sp. possesses an ability to degrade NP and can serve as a potential candidate for further biodegradation studies.
Physical description
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
  • Department of Systematics and Plant Geography, Faculty of Biology, University of Warsaw, Warsaw, Poland
  • Department of Systematics and Plant Geography, Faculty of Biology, University of Warsaw, Warsaw, Poland
  • Department of Plant Protection, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland
  • Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland
  • Amóra SS, Bevilaqua CM, Feijó FM, Pereira RH, Alves ND, Freire FA, Kamimura MT, de Oliveira DM, Luna-Alves Lima EA, Rocha M (2010) The effects of the fungus Metarhizium anisopliae var. acridum on different stages of Lutzomyia longipalpis (Diptera: Psychodidae). Acta Trop 113: 214-220.
  • Bernat P, Długoński J (2012) Comparative study of fatty acids composition during cortexolone hydroxylation and tributyltin chloride (TBT) degradation in the filamentous fungus Cunninghamella elegans. Int Biodeterior Biodegradation 74: 1-6.
  • Bidochka MJ, Kamp AM, Lavender TM, Dekoning J, De Croos JN (2001) Habitat association in two genetic groups of the insect-pathogenic fungus Metarhizium anisopliae: uncovering cryptic species? Appl Environ Microbiol 67: 1335-1342.
  • Bischoff JF, Rehner SA, Humber RA (2006) Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia 98: 737-745.
  • Bischoff JF, Rehner SA, Humber RA (2009) A multilocus phylogeny of the Metarhizium anisopliae lineage. Mycologia 101: 512-530.
  • Brown S, Devin-Clarke D, Doubrava M, O'Connor G (2009) Fate of 4-nonylphenol in a biosolids amended soil. Chemosphere 75: 549-554.
  • Budziszewska J, Szypuła W, Wilk M, Wrzosek M (2011) Paraconiothyrium babiogorense sp. nov., a new endophyte from fir club moss Huperzia selago (Huperziaceae). Mycotaxon 115: 457-468.
  • Bruck DJ (2009) Impact of fungicides on Metarhizium anisopliae in the rhizosphere, bulk soil and in vitro. BioControl 54: 597-606.
  • Cajthaml T, Kresinova Z, Svobodova K, Moder M (2009) Biodegradation of endocrine-disrupting compounds and suppression of estrogenic activity by ligninolytic fungi. Chemosphere 75: 745-750.
  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553-555.
  • Domene X, Ramírez W, Solà L, Alcañiz JM, AndrésP (2009) Soil pollution by nonylphenol and nonylphenol ethoxylates and their effects to plants and invertebrates. J Soils Sediments 9: 555-567.
  • Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792-1797.
  • Fernandes ÉK, Bittencourt VR, Roberts DW (2012) Perspectives on the potential of entomopathogenic fungi in biological control of ticks. Exp Parasitol 130: 300-305.
  • Gouy M, Guindon S, Gascuel O (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27: 221-224.
  • Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59: 307-321.
  • Jeon CO, Madsen EL (2012) In situ microbial metabolism of aromatic-hydrocarbon environmental pollutants. Curr Opin Biotechnol 24: 474-481.
  • Kaszycki P, Czechowska K, Petryszak P, Miedzobrodzki J, Pawlik B, Kołoczek H. (2006) Methylotrophic extremophilic yeast Trichosporon sp.: a soil-derived isolate with potential applications in environmental biotechnology. Acta Biochim Pol 53: 463-473.
  • Kepler RM, Rehner SA (2013) Genome-assisted development of nuclear intergenic sequence markers for entomopathogenic fungi of the Metarhizium anisopliae species complex. Mol Ecol Resour 13: 210-217.
  • Kinney CA, Campbell BR, Thompson R, Furlong ET, Kolpin DW, Burkhardt MR, Zaugg SD, Werner SL, Hay AG (2012) Earthworm bioassays and seedling emergence for monitoring toxicity, aging and bioaccumulation of anthropogenic waste indicator compounds in biosolids-amended soil. Sci Total Environ 433: 507-515.
  • Langdon KA, Warne MS, Smernik RJ, Shareef A, Kookana RS (2011) Degradation of 4-nonylphenol, 4-t-octylphenol, bisphenol A and triclosan following biosolids addition to soil under laboratory conditions. Chemosphere 84: 1556-1562.
  • Langdon KA, Warne MS, Smernik RJ, Shareef A, Kookana RS (2012) Field dissipation of 4-nonylphenol, 4-t-octylphenol, triclosan and bisphenol A following land application of biosolids. Chemosphere 86: 1050-1058.
  • Lisowska K, Szemraj J, Różalska S, Długoński J (2006) The expression of cytochrome P-450 and cytochrome P-450 reductase genes in the simultaneous transformation of corticosteroids and phenanthrene by Cunninghamella elegans. FEMS Microbiol Lett 261: 175-180.
  • Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M (2001) Biological control of locusts and grasshoppers. Ann Rev Entomol 46: 667-702.
  • Luque J, Martos S, Phillips AJ (2005) Botryosphaeria viticola sp. nov. on grapevines: a new species with a Dothiorella anamorph. Mycologia 97: 1111-1121.
  • Milner RJ (1992) Selection and characterization of strains of Metarhizium anisopliae for control of soil insects in Australia. In: Biological Control of Locusts and Grasshoppers. Lomer CJ, Prior C eds, pp 200-207. CAB International, Wallingford.
  • Ojeda G, Patrício J, Navajas H, Comellas L, Alcañiz JM, Ortiz O, Marks E, Natal-da-Luz T, Sousa JP (2013) Effects of nonylphenols on soil microbial activity and water retention. App Soil Ecol 64: 77-83.
  • Roberts DW, St Leger RJ (2004) Metarhizium spp., cosmopolitan insect-pathogenic fungi: mycological aspects. Adv Appl Microbiol 54: 1-70.
  • Rocha LF, Inglis PW, Humber RA, Kipnis A, Luz C (2012) Occurrence of Metarhizium spp. in Central Brazilian soils. J Basic Microbiol 53: 251-259.
  • Różalska S, Szewczyk R, Długoński J (2010) Biodegradation of 4-n-nonylphenol by the non-ligninolytic filamentous fungus Gliocephalotrichum simplex: a proposal of a metabolic pathway. J Hazard Mater 180: 323-331.
  • Schneider S, Rehner SA, Widmer F, Enkerli J (2011) A PCR-based tool for cultivation-independent detection and quantification of Metarhizium clade 1. J Invertebr Pathol 108: 106-114.
  • Schumacher V, Poehling HM (2012) In vitro effect of pesticides on the germination, vegetative growth, and conidial production of two strains of Metarhizium anisopliae. Fungal Biol 116: 121-132.
  • Słaba M, Szewczyk R, Bernat P, Długoński J (2009) Simultaneous toxic action of zinc and alachlor resulted in enhancement of zinc uptake by the filamentous fungus Paecilomyces marquandii. Sci Total Environ 407: 4127-4133.
  • White TJ, Bruns TD, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a guide to methods and applications. Innis MA, Gelfand DH, Sninsky JJ, White TJ eds, pp 315-322. Academic Press, New York.
  • Vallini G, Frassinetti S, Scorzetti G (1997) Candida aquaetextoris sp. nov., a new species of yeast occurring in sludge from a textile industry wastewater treatment plant in Tuscany, Italy. Int J Syst Bacteriol 47: 336-340.
  • Wyrebek M, Huber C, Sasan RK, Bidochka MJ (2011) Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity. Microbiology 157: 2904-2911.
  • Venkatesan AK, Halden RU (2013) National inventory of alkylphenol ethoxylate compounds in U.S. sewage sludges and chemical fate in outdoor soil mesocosms. Environ Pollut 174: 189-193.
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