Apoptosis of peripheral blood leucocytes in rabbits infected with different strains of rabbit haemorrhagic disease virus
Languages of publication
The pathogenicity of RHDV (rabbit haemorrhagic disease virus) is mainly associated with its affinity to blood vessels, with causing disseminated intravascular coagulations (DIC), and with the stimulation of the host immune system. Moreover, there are implications suggesting that apoptosis may be a pivotal process in understanding the basis of viral haemorrhagic disease in rabbits - a serious infectious disease causing mortality to wild and domestic rabbits. The aim of this study is to evaluate, by means of flow cytometry, the dynamics of apoptosis in peripheral blood granulocytes and lymphocytes in rabbits experimentally infected with seven different strains of RHDV and so-called antigenic variants of RHDV denominated as RHDVa, i.e.: Hungarian 24V/89, 1447V/96, 72V/2003; Austrian 01-04, 237/04, V-412 and French 05-01. The results showed that all of the RHDV and RHDVa strains cause an increase in the number of apoptotic cells throughout the infection, which might indicate the need for further analysis of the importance of this process.
- Alonso C, Oviedo JM, Martin-Alonso JM, Diaz E, Boga JA, Parra F (1998) Programmed cell death in the pathogenesis of rabbit hemorrhagic disease. Arch Virol 143: 321-332.
- Anon (1987) Information and training materials of the Laboratory Animals Section, General Assembly of the Association of Agriculture Engineers and Technicians, p. 26-77 (in Polish).
- Anon (2010) Regulation of the Minister of Agriculture and Rural Development of 10 March 2006 on detailed conditions for maintenance of laboratory animals in experimental units, breeding units and suppliers (Polish Journal of Laws of 2006, No. 50, item 368 (in Polish).
- Garcia-Lastra R, San-Miguel B, Crespo I, Jorquera F, Alvarez M, Gonzalez-Gallego J, Tunon M (2010) Signaling pathways involved in liver injury and regeneration in rabbit hemorrhagic disease, an animal model of virally-induced fulminant hepatic failure. Vet Res 41: 2.
- Jung JY, Lee BJ, Tai JH, Park JH, Lee YS (2000) Apoptosis in Rabbit Haemorrhagic Disease. J Comp Path 123: 135-140.
- Marques RM, Costa-e-Silva A, Águas AP, Teixeira L, Ferriera PG (2010) Early acute depletion of lymphocytes in calicivirus-infected adult rabbits. Vet Res Commun 34: 659-668.
- Ni Z, Wei W, Liu GQ, Chen L, Yu B, Yun T, Hua JG, Li SM (2009) Studies on the apoptosis of RK13cells induced by rabbit hemorrhagic disease virus. Chin J Virol 25: 316-317.
- Niedźwiedzka-Rystwej P, Deptuła W (2010) Non-specific immunity in rabbits infected with 10 strains of the rabbit haemorrhagic disease virus with different biological properties. Centr Europ J Biol 5: 613-632.
- Niedźwiedzka-Rystwej P, Deptuła W (2012) Apoptosis of peripheral blood leukocytes from rabbits infected with non-haemagglutinating strains of rabbit haemorrhagic disease virus (RHDV). Vet Immunol Immunopathol 149: 54-57.
- Niedźwiedzka-Rystwej P, Tokarz-Deptuła B, Deptuła W (2013) The phenomenon of apoptosis of granulocytem and lymphocytes in peripheral blood in rabbits infected with haemagglutinating and non-haemagglutinating antigenic variants of the RHD virus (rabbit haemorrhagic disease). Pol J Vet Sci (in press).
- Nyström K, Le Gall-Recule G, Grassi P, Abrantes J, Ruvoën-Clouet N, Le Moullac-Vaidye B, Lopes AM, Esteves PJ, Strive T, Marchandeau S, Haslam SM, Le Pendu J (2011) Histo-blood group antigens act as attachment factors of rabbit hemorrhagic disease virus infection in a virus strain-dependent manner. PLOS Pathog 7: e1002188.
- San-Miguel B, Alvarez M, Culebras JM, Gonzalez-Gallego J, Tunon MJ (2006) N-acetyl-cysteine protects liver from apoptotic death in an animal model of fulminant hepatic failure. Apoptosis 11: 1945-1957.
- Tokarz-Deptuła B (2009) Immunity phenomena in rabbits infected with the RHD (rabbit haemorrhagic disease) virus. Pol J Env Stud 7: 1-81.
- Tuñón MJ, San Miguel B, Crespo I, Jorquera F, Santamaria E, Prieto J, González-Gallego J (2011) Melatonin attenuates apoptotic liver damage in fulminant hepatic failure induced by rabbit hemorrhagic disease virus. J Pineal Res 50: 38-45.
- Ueda K, Park HI, Ochiai K, Itakura C (1992) Disseminated intravascular coagulation (DIC) in rabbit haemorrhagic disease. Jpn J Vet Res 40: 133-141.
Publication order reference