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2013 | 60 | 4 | 657-660
Article title

Generation of stable, non-aggregating Saccharomyces cerevisiae wild isolates

Content
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Languages of publication
EN
Abstracts
EN
Cellular aggregates observed during growth of Saccharomyces cerevisiae strains derived from various natural environments makes most laboratory techniques optimized for non-aggregating laboratory strains inappropriate. We describe a method to reduce the size and percentage of the aggregates. This is achieved by replacing the native allele of the AMN1 gene with an allele found in the W303 laboratory strain. The reduction in aggregates is consistent across various environments and generations, with no change in maximum population density or strain viability, and only minor changes in maximum growth rate and colony morphology.
Year
Volume
60
Issue
4
Pages
657-660
Physical description
Dates
published
2013
received
2013-09-25
revised
2013-11-22
accepted
2013-11-25
(unknown)
2013-12-20
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p657kz
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