PL EN


Preferences help
enabled [disable] Abstract
Number of results
2013 | 60 | 4 | 635-640
Article title

AS-30D hepatoma as a model to study on insulin resistance in vitro

Content
Title variants
Languages of publication
EN
Abstracts
EN
Studies on insulin resistance of liver cells are often performed with the use of various hepatoma cell lines. Such an approach allows investigating selected biochemical pathways at the cellular level. However, possible modifications of metabolic processes due to the neoplastic nature of such cells must be considered. Expanding the diversity of hepatoma cell lines used in metabolic studies could deliver new data for comparison with those obtained for other cell lines and should reduce the risk of misleading conclusions. In this study rat hepatoma AS-30D cells were tested as a potential model for studies on palmitate-induced insulin resistance. It was found that insulin-induced Akt kinase phosphorylation was substantially reduced in cells incubated with palmitate at a concentration as low as 75 µM. This effect was not accompanied by excessive reactive oxygen species (ROS) generation or increased Jun N-terminal kinase (JNK) phosphorylation. Moreover, preincubation of AS-30D cells with rosiglitazone, an antidiabetic agonist of peroxisome proliferator-activated receptor gamma (PPARγ), efficiently prevented the palmitate-induced insulin resistance. We conclude that AS-30D hepatoma cells may be used as a model sensitive to insulin and vulnerable to palmitate-induced insulin resistance.
Publisher

Year
Volume
60
Issue
4
Pages
635-640
Physical description
Dates
published
2013
received
2013-08-20
revised
2013-10-12
accepted
2013-10-27
(unknown)
2013-11-22
Contributors
  • Nencki Institute of Experimental Biology, Warsaw, Poland
  • Nencki Institute of Experimental Biology, Warsaw, Poland
  • Nencki Institute of Experimental Biology, Warsaw, Poland
  • Nencki Institute of Experimental Biology, Warsaw, Poland
  • Nencki Institute of Experimental Biology, Warsaw, Poland
References
  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-917.
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
  • Briscoe DA, Fiskum G, Holleran AL, Kelleher JK (1994) Acetoacetate metabolism in AS-30D hepatoma cells. Mol Cell Biochem 136: 131-137.
  • Burgermeister E, Schnoebelen A, Flament A, Benz J, Stihle M, Gsell B, Rufer A, Ruf A, Kuhn B, Märki HP, Mizrahi J, Sebokova E, Niesor E, Meyer M (2006) A novel partial agonist of peroxisome proliferator-activated receptor-gamma (PPARgamma) recruits PPARgamma-coactivator-1alpha, prevents triglyceride accumulation, and potentiates insulin signaling in vitro. Mol Endocrinol 20: 809-830.
  • Cao J, Dai DL, Yao L, Yu HH, Ning B, Zhang Q, Chen J, Cheng WH, Shen W, Yang ZX (2012) Saturated fatty acid induction of endoplasmic reticulum stress and apoptosis in human liver cells via the PERK/ATF4/CHOP signaling pathway. Mol Cell Biochem 364: 115-129.
  • Chen Z, Vigueira PA, Chambers KT, Hall AM, Mitra MS, Qi N, McDonald WG, Colca JR, Kletzien RF, Finck BN (2012) Insulin Resistance and metabolic derangements in obese mice are ameliorated by a novel peroxisome proliferator-activated receptor -sparing thiazolidinedione. J Biol Chem 287: 23537-23548.
  • Cousin SP, Hugl SR, Wrede CE, Kajio H, Myers MG, Rhodes CJ (2001) Free fatty acid-induced inhibition of glucose and insulin-like growth factor I-induced deoxyribonucleic acid synthesis in the pancreatic beta-cell line INS-1. Endocrinology 142: 229-240.
  • Djaouti L, Jourdan T, Demizieux L, Chevrot M, Gresti J, Vergès B, Degrace P (2010) Different effects of pioglitazone and rosiglitazone on lipid metabolism in mouse cultured liver explants. Diabetes Metab Res Rev 26: 297-305.
  • Feng XT, Wang TZ, Leng J, Chen Y, Liu JB, Liu Y, Wang WJ (2012) Palmitate contributes to insulin resistance through downregulation of the Src-mediated phosphorylation of Akt in C2C12 myotubes. Biosci Biotechnol Biochem 76: 1356-1361.
  • Gao D, Nong S, Huang X, Lu Y, Zhao H, Lin Y, Man Y, Wang S, Yang J, Li J (2010) The effects of palmitate on hepatic insulin resistance are mediated by NADPH oxidase 3-derived reactive oxygen species through JNK and p38MAPK pathways. J Biol Chem 285: 29965-29973.
  • Ginter E, Simko V (2012) Type 2 diabetes mellitus, pandemic in 21st century. Adv Exp Med Biol 771: 42-50.
  • Guo L, Zhang L, Sun Y, Muskhelishvili L, Blann E, Dial S, Shi L, Schroth G, Dragan YP (2006) Differences in hepatotoxicity and gene expression profiles by anti-diabetic PPARγ agonists on rat primary hepatocytes and human HepG2 cells. Mol Divers 10: 349-360.
  • Henriksen EJ, Diamond-Stanic MK, Marchionne EM (2010) Oxidative stress and the etiology of insulin resistance and type 2 diabetes. Free Radic Biol Med 5: 993-999.
  • Holleran AL, Briscoe DA, Fiskum G, Kelleher JK (1995) Glutamine metabolism in AS-30D hepatoma cells. Evidence for its conversion into lipids via reductive carboxylation. Mol Cell Biochem 152: 95-101.
  • Jiang G, Dallas-Yang Q, Biswas S, Li Z, Zhang BB (2004) Rosiglitazone, an agonist of peroxisome-proliferator-activated receptor gamma (PPARgamma), decreases inhibitory serine phosphorylation of IRS1 in vitro and in vivo. Biochem J 377 (Pt2): 339-346.
  • Kumar N, Dey CS (2003) Development of insulin resistance and reversal by thiazolidinediones in C2C12 skeletal muscle cells. Biochem Pharmacol 65: 249-257.
  • Lee JY, Cho HK, Kwon YH (2010) Palmitate induces insulin resistance without significant intracellular triglyceride accumulation in HepG2 cells. Metab Clin Expt 59: 927-934.
  • Mathupala SP, Rempel A, Pedersen PL (1995) Glucose catabolism in cancer cells. J Biol Chem 270: 16918-16925.
  • Mordier S, Iynedjian PB (2007) Activation of mammalian target of rapamycin complex 1 and insulin resistance induced by palmitate in hepatocytes. Biochem Biophys Res Commun 362: 206-211.
  • Nakamura S, Takamura T, Matsuzawa-Nagata N, Takayama H, Misu H, Noda H, Nabemoto S, Kurita S, Ota T, Ando H, Miyamoto K, Kaneko S (2009) Palmitate induces insulin resistance in H4IIEC3 hepatocytes through reactive oxygen species produced by mitochondria. J Biol Chem 284: 14809-14818.
  • Rodríguez-Enríquez S, Juárez O, Rodríguez-Zavala JS, Moreno-Sánchez R (2001) Multisite control of the Crabtree effect in ascites hepatoma cells. Eur J Biochem 268: 2512-2519.
  • Rogue A, Spire C, Brun M, Claude N, Guillouzo A (2010) Gene expression changes induced by PPAR gamma agonists in animal and human liver. PPAR Res doi: 10.1155/2010/325183.
  • Ruddock MW, Stein A, Landaker E, Park J, Cooksey RC, McClain D, Patti ME (2008) Saturated fatty acids inhibit hepatic insulin action by modulating insulin receptor expression and post-receptor signalling. J Biochem 144: 599-607.
  • Snel M, Jonker JT, Schoones J, Lamb H, de Roos A, Pijl H, Smit JW, Meinders AE, Jazet IM (2012) Ectopic fat and insulin resistance: pathophysiology and effect of diet and lifestyle interventions. Int J Endocrinol doi: 10.1155/2012/983814.
  • Walborg EF Jr, Christenson L, Chang JP (1970) Establishment of a Transplantable Ascites Variant of a Rat Hepatoma Induced by 3'-Methyl-4-dimcthylaminoazobenzene. Cancer Res 30: 2306-2309.
  • Wu HS, Yu JH, Li YY, Yang YS, He QJ, Lou YJ, Ji RY (2007) Insulin-sensitizing effects of a novel a-methyl-a-phenoxylpropionate derivative in vitro. Acta Pharmacol Sin 28: 417-422.
  • Yang C, Aye CC, Li X, Diaz Ramos A, Zorzano A, Mora S (2012) Mitochondrial dysfunction in insulin resistance: differential contributions of chronic insulin and saturated fatty acid exposure in muscle cells. Biosci Rep 32: 465-478.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p635kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.