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2013 | 60 | 3 | 489-493
Article title

The alcohol dehydrogenase isoenzyme (ADH IV) as a candidate tumour marker of esophageal cancer

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Abstracts
EN
Objective: Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are present in esophageal cancer cells. Moreover the total activity of ADH as well as the activity of class IV ADH isoenzyme is significantly higher in cancer tissue than in healthy mucosa. The activity of these enzymes in cancer cells is reflected in the sera and could thus be helpful for diagnostics of esophageal cancer. The aim of this study was to investigate a potential significance of ADH isoenzymes and ALDH as tumour markers of esophageal cancer. We defined diagnostic sensitivity, specificity, predictive value for positive and negative results, and receiver-operating characteristics (ROC) curve for tested enzymes. Methods: Serum samples were taken for routine biochemical investigation from 180 patients with esophageal cancer before treatment. Total ADH activity was measured by a photometric method with p-nitrosodimethylaniline as a substrate and ALDH activity by a fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by a photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. Results: There was a significant increase in the activity of class IV of ADH isoenzyme (7.65 mU/l vs 5.88 mU/l) and total ADH activity (1198 mU/l vs 848 mU/l) in the sera of esophageal cancer patients compared to the control. The diagnostic sensitivity for ADH IV was 72%, the specificity 76%, the positive and negative predictive values were 80% and 72% respectively. The area under the ROC curve for ADH IV was 0.65. Conclusion: The results suggest a potential significance of ADH IV as a marker of esophageal cancer.
Publisher

Year
Volume
60
Issue
3
Pages
489-493
Physical description
Dates
published
2013
received
2013-06-19
revised
2013-09-04
accepted
2013-09-11
(unknown)
2013-09-19
Contributors
  • Department of Biochemical Diagnostics, Medical University, Białystok, Poland
  • Department of Biochemical Diagnostics, Medical University, Białystok, Poland
  • Department of Thoracic Surgery, Medical University, Białystok, Poland
  • Department of Thoracic Surgery, Medical University, Białystok, Poland
  • Department of Thoracic Surgery, Medical University, Białystok, Poland
  • Department of Biochemical Diagnostics, Medical University, Białystok, Poland
References
  • Estonius M, Svensson S, Hoog JO (1996) Alcohol dehydrogenase in human tissue: localisation of transcripts coding for five classes of the enzyme. FEBS Lett 397: 338-42.
  • Dohmen K, Baraona E, Ishibashi H (1996) Ethnic differences in gastric s-alcohol dehydrogenase activity and ethanol first pass metabolism. Alcohol Clin Exp Res 20: 1569-1576.
  • Goedde HW, Agarwal DP (1987) Polymorphism of aldehyde dehydrogenase and alcohol sensitivity. Enzyme 37: 29-44.
  • Guillem P, Triboulet J (2005) Elevated serum levels of C-reactive protein are indicative of a poor prognosis in patients with esophageal cancer. Dis Esophagus 18: 146-150.
  • Jelski W, Chrostek L, Zalewski B, Szmitkowski M (2008a) Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with gastric cancer. Dig Dis Sci 53: 2101-2105.
  • Jelski W, Kozlowski M, Laudanski J, Niklinski J, Szmitkowski M (2009a) The activity of class I, II, III, and IV alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in esophageal cancer. Dig Dis Sci 54: 725-730.
  • Jelski W, Kozlowski M, Laudanski J, Niklinski J, Szmitkowski M (2009b) Alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in the sera of patients with esophageal cancer. Clin Exp Med 9: 131-137.
  • Jelski W, Kutylowska E, Laniewska-Dunaj M, Szmitkowski M (2011) Alcohol dehydrogenase (ADH) and aldehyde dehydrpgenase (ALDH) as candidates for tumor markers in patients with pancreatic cancer. J Gastrointestin Liver Dis 20: 255-259.
  • Jelski W, Mroczko B, Szmitkowski M (2010b) The diagnostic value of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) measurement in the sera of colorectal cancer patients. Dig Dis Sci 55: 2953-2957.
  • Jelski W, Orywal K, Laniewska M, Szmitkowski M (2010a) The diagnostic value of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) measurement in the sera of gastric cancer patients. Clin Exp Med 10: 215-219.
  • Jelski W, Zalewski B, Szmitkowski M (2008b) Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with pancreatic cancer. Dig Dis Sci 53: 2276-2280.
  • Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011) Global cancer statistics. CA Cancer J Clin 61: 69-90.
  • Kairisto V, Virtanen A, Uusipaikka E, Voipio-Pulkki LM, Näntö V, Peltola O, Irjala K (1993) Method for determining reference changes from patients' serial data: example of cardiac enzymes. Clin Chem 39: 2298-2304.
  • Koivusalo M, Baumann M, Uotila L (1989) Evidence for the identity of glutathione- dependent formaldehyde dehydrogenase and class III alcohol dehydrogenase. FEBS Lett 257: 105-109.
  • Lindblom A, Liljegren A (2000) Tumour markers in malinancies. BMJ 320: 424-427.
  • Lukaszewicz-Zajac M, Mroczko B, Kozlowski M, Niklinski J, Laudanski J, Szmitkowski M (2010) Clinical significance of serum macrophage-colony stimulating factor (M-CSF) in esophageal cancer patients and its comparison with classical tumor markers. Clin Chem Lab Med 48: 1467-1473.
  • Lukaszewicz-Zajac M, Mroczko B, Kozlowski M, Niklinski J, Laudanski J, Szmitkowski M (2012) Higher importance of interleukine 6 (IL-6) than classical tumor markers (CEA and SCC-Ag) in the diagnosis of esophageal cancer patients. Dis Esophagus 25: 242-249.
  • Mroczko B, Kozlowski M, Groblewska M, Lukaszewicz M, Niklinski J, Jelski W, Laudanski J, Chyczewski L, Szmitkowski M (2008) The diagnostic value of measurement of matrix metalloproteinase 9 (MMP-9), squamos cell cancer antigen (SCC) and carcinoembryonic antigen (CEA) in the sera of esophageal cancer patients. Clin Chim Acta 389: 61-66.
  • Skursky L, Kovar J, Stachova M (1979) A sensitive photometric assay for alcohol dehydrogenase activity in blood serum. Anal Biochem 99: 65-71.
  • Vaglenova J, Martinez SE, Porte S, Duester G, Farres J, Pares X (2003) Expression, localization and potential physiological significance of alcohol dehydrogenase in the gastrointestinal tract. Eur J Biochem 270: 2652-2662.
  • Vallbohmer D, Lenz HJ (2006) Predictive and prognostic molecular markers in outcome of esophageal cancer. Dis Esophagus 19: 425-432.
  • Wierzchowski J, Dafeldecker WP, Holmquist B, Vallee BL (1989) Fluorometric assays for isozymes of human alcohol dehydrogenase. Anal Biochem 178: 57-62.
  • Yin S-J, Chou F-J, Chao S-F, Tsai S-F, Liao C-S, Wang S-L, Wu C-W, Lee S-C (1993) Alcohol and aldehyde dehydrogenases in human esophagus: comparison with the stomach enzyme activities. Alcohol Clin Exp Res 17: 376-381.
Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p489kz
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