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2013 | 60 | 3 | 485-488
Article title

Homocysteine thiolactone affects protein ubiquitination in yeast

Content
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EN
Abstracts
EN
The formation of homocysteine thiolactone (HcyTl) from homocysteine occurs in all examined so far organisms including bacteria, yeast, and humans. Protein N-homocysteinylation at the ε-amino group of lysine is an adverse result of HcyTl accumulation. Since tagging of proteins by ubiquitination before their proteasomal degradation takes place at the same residue, we wondered how N-homocysteinylation may affect the ubiquitination of proteins. We used different yeast strains carrying mutations in genes involved in the homocysteine metabolism. We found positive correlation between the concentration of endogenous HcyTl and the concentration of ubiquitinated proteins. This suggests that N-homocysteinylation of proteins apparently does not preclude but rather promotes their decomposition.
Publisher

Year
Volume
60
Issue
3
Pages
485-488
Physical description
Dates
published
2013
received
2013-06-13
revised
2013-08-30
accepted
2013-09-09
(unknown)
2013-09-19
Contributors
author
  • Poznań University of Life Sciences, Poznań, Poland
  • Poznań University of Life Sciences, Poznań, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p485kz
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