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2013 | 60 | 1 | 43-50
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Composition of volatile in micropropagated and field grown aromatic plants from Tuscany Islands

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Aromatic plant species present in the natural Park of Tuscany Archipelago are used as flavoring agents and spices, as dietary supplements and in cosmetics and aromatherapy. The plants are usually collected from wild stands, inducing a depletion of the natural habitat. Therefore, micropropagation of these aromatic plants can play a role in the protection of the natural ecosystem, can guarantee a massive sustainable production and can provide standardized plant materials for diverse economical purposes. The aim of this study is to compare the volatile organic compounds produced by the wild plants with those from in vitro plantlets using headspace solid phase micro-extraction (HS-SPME) followed by capillary gas-chromatography coupled to mass spectrometry (GC-MS). Typical plants of this natural area selected for this work were Calamintha nepeta L., Crithmum maritimum L., Lavandula angustifolia L., Myrtus communis L., Rosmarinus officinalis L., Salvia officinalis L. and Satureja hortensis L. Different explants were used: microcuttings with vegetative apical parts, axillary buds and internodes. Sterilization percentage, multiplication rate and shoot length, as well as root formation were measured. The volatile aromatic profiles produced from in vitro plantlets were compared with those of the wild plants, in particular for C. maritimum, R. officinalis, S. officinalis and S. hortensis. This study indicated that the micropropagation technique can represent a valid alternative to produce massive and sterile plant material characterised by the same aromatic flavour as in the wild grown plants.
Physical description
  • Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy
  • Department of Pharmacy, University of Pisa, Pisa, Italy
  • Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy
  • Department of Pharmacy, University of Pisa, Pisa, Italy
  • Abraham Z (2011) Collection and conservation of medicinal and aromatic plants resources. OAJMAP 1: 40-43.
  • Abu-Lafi S, Odeh I, Dewik H, Qabajah M, Hanus LO, Dembitsky VM (2008) Thymol and carvacrol production from leaves of wild palestinian Majorana syriaca. Bioresour Technol 99: 3914-3918.
  • Adams RP (1995) In Identification of essential oil components by gas chromatography-mass spectroscopy. Allured, Carol Stream, Illinois.
  • Andrade LB, Echeverrigaray S, Fracaro F, Pauletti GF, Rota L (1999) The effect of growth regulators on shoot propagation and rooting of common lavender (Lavandula vera DC). Plant Cell Tissue Org Cult 56: 79-83.
  • Arikat NA, Jawad M, Karam NS, Shibli RA (2004) Micropropagation and accumulation of essential oils in wild sage (Salvia fruticosa Mill.) Sci Hortic 100: 193-202.
  • Arrebola ML, Socorro O, Barceló-Muñoz A, Simón-Pérez E, Pliego-Alfaro F (1997) Micropropagation of Satureja obovata Lag. Hort Sci 32: 1278-1280.
  • Avato P, Morone Fortunato I, Ruta C, D'Elia R (2005) Glandular hair's and essential oils in micropropagated plants of Salvia officinalis L. Plant Sci 169: 29-36.
  • Azaz AD, Kürkcüoglu M, Satil F, Can Baser KH, Tümen G (2005) In vitro antimicrobial activity and chemical composition of some Satureja essential oils. Flavour Fragr J 20: 587-591.
  • Belliardo F, Bicchi C, Cordero C, Liberto E, Rubiolo P, Sgorbini (2006) Headspace-solid-phase microextraction in the analysis of the volatile fraction of aromatic and medicinal plants. J Chromatogr Sci 44: 416-429.
  • Connolly JD, Hill RA (1991) In Dictionary of terpenoids. Chapman & Hall, London.
  • Da Porto C, Decorti D (2008) Analysis of the volatile compounds of flowers and essential oils from Lavandula angustifolia cultivated in northeastern italy by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry. Planta Med 74: 182-187.
  • De Pooter HL, De Buyck LF, Schamp NM (1986) The volatiles of Calamintha nepeta subsp. glandulosa. Phytochemistry 25: 691-694.
  • Debnath M, Malik CP, Bisen PS (2006) Micropropagation: a tool for the production of high quality plant-based medicines. Curr Pharm Biotechnol 7: 33-49.
  • Dicke M, Loreto F (2010) Induced plant volatiles: from genes to climate change. Trends Plant Sci 15: 115-117.
  • Flamini G, Cioni PL, Morelli I, Maccioni S, Baldini R (2004) Phytochemical typologies in some populations of Myrtus communis L. on Caprione Promontory (East Liguria, Italy). Food Chem 85: 599-604.
  • Gonçalves S, Serra H, Nogueira JMF, Almeida R, Custódio L, Romano A (2008) Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis. Biol Plantarum 52: 133-136.
  • Grigoriadou K, Maloupa E (2008) Micropropagation and salt tolerance of in vitro grown Crithmum maritimum L. Plant Cell Tiss Org Cult 94: 209-217.
  • Güllüce M, Sökmen M, Daferera D, Agar G, Özkan H, Kartal N, Polissiou M, Sökmen A, Sahin F (2003) In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. J Agric Food Chem 51: 3958-3965.
  • Hamilton AC (2004) Medicinal plants, conservation and livelihoods. Biodivers Conserv 13: 1477-1517.
  • Jennings W, Shibamoto T (1980) In Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. Academic Press, New York.
  • Katerinopoulos HE, Pagona G, Afratis A, Stratigakis N, Roditakis N (2005) Composition and insect attracting activity of the essential oil of Rosmarinus officinalis. J Chem Ecol 31: 111-122.
  • Kim NS, Lee DD (2002) Comparison of different extraction method for the analysis of fragrance from Lavandula species by gas chromatographymass spectrometry. J Chromatogr A 982: 31-47.
  • Kulisic-Bilusic T, Blazevic I, Dejanovic B, Milos M, Pifat G (2010) Evaluation of the antioxidant activity of essential oils from caper (Capparis spinosa) and sea fennel (Crithmum maritimum) by different methods. J Food Biochem 34: 286-302.
  • Landi S (1989) In Flora e ambiente dell'isola d'Elba. Editrice Azzurra, Cavalese (Tn).
  • Longaray Delamare AP, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S (2007) Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem 100: 603-608.
  • Lucchesini M, Mensuali-Sodi A (2010) Plant tissue culture-An opportunity for the production of nutraceuticals. Adv Exp Med Biol 698: 185-202.
  • Maffei M, Gertsch J, Appendino G (2011) Plant volatiles: production, function and pharmacology. Nat Prod Rep 28: 1359-1380.
  • Massada Y (1976) In Analysis of essential oils by gas chromatography and mass spectrometry. Wiley & Sons, New York.
  • Misra P, Chaturvedi HC (1984) Micropropagation of Rosmarinus officinalis L. Plant Cell Tiss Org Cult 3: 163-168.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-493.
  • Özcan MM, Pedro LG, Figueiredo AC, Barroso JG (2006) Constituents of the essential oil of sea fennel (Crithmum maritimum L.) growing wild in Turkey. J Med Food 9: 128-130.
  • Pistelli L, Giovannini A, Ruffoni B, Bertoli A, Pistelli L. (2010) Hairy root cultures for secondary metabolites production. Adv Exp Med Biol 698: 167-184.
  • Rinaldi G (2000) In Flora dell'Arcipelago Toscano. Edizioni Archipelagos, Portoferraio (Livorno).
  • Ruffoni B, Pistelli L, Bertoli A, Pistelli L (2010) Plant Cell Cultures: Bioreactors for Industrial Production. Adv Exp Med Biol 698: 203-221.
  • Ruffoni B, Mascarello C (2009) Tecniche per la propagazione in vitro degli arbusti mediterranei. Flortecnica 6: 51-52.
  • Santos-Gomes PC, Fernandes-Ferreira M (2001) Organ- and season-dependent variation in the essential oil composition of Salvia officinalis L. cultivated at two different sites. J Agric Food Chem 49: 2908-2916.
  • Scarpa GM, Milia M, Satta M (2000) The influence of growth regulators on proliferation and rooting of in vitro propagated myrtle Plant Cell Tiss Org Cult 62: 175-179.
  • Sefidkon F, Abbasi K, Khaniki GB (2006) Influence of drying and extraction methods on yield and chemical composition of the essential oil of Satureja hortensis. Food Chem 99: 19-23.
  • Stenhagen E, Abrahamsson S, McLafferty FW (1974) In Registry of mass spectral data. Wiley & Sons, New York.
  • Swigar AA, Silverstein RM (1981) In Monoterpenes. Aldrich Chem. Comp., Milwaukee.
  • Zawirska-Wojtasiak R, Wasowicz E (2009) GC analysis of rosemary aroma isolated traditionally by distillation and by SPME. J Essent Oil Res 21: 8-15.
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