A simple method for the determination of the cholesterol esterase activity

• Authors: Agnieszka Stępień , Mykhailo Gonchar
• Languages of publication: EN

Abstracts

EN

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

Keywords

EN

cholesterol esterase; cholesterol; activity determination; human serum

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 3
• Pages: 401-403

Dates

published

2013

received

2012-11-28

revised

2013-07-25

accepted

2013-09-13

(unknown)

2013-09-20

Contributors

author

Agnieszka Stępień

• Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland

author

Mykhailo Gonchar

• Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland

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