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2013 | 60 | 3 | 401-403
Article title

A simple method for the determination of the cholesterol esterase activity

Content
Title variants
Languages of publication
EN
Abstracts
EN
The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.
Publisher

Year
Volume
60
Issue
3
Pages
401-403
Physical description
Dates
published
2013
received
2012-11-28
revised
2013-07-25
accepted
2013-09-13
(unknown)
2013-09-20
Contributors
  • Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland
  • Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland
References
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  • Sigma quality control test procedure
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p401kz
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