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2013 | 60 | 3 | 395-400
Article title

Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

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Languages of publication
EN
Abstracts
EN
Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.
Year
Volume
60
Issue
3
Pages
395-400
Physical description
Dates
published
2013
received
2012-10-08
revised
2013-06-28
accepted
2013-07-08
(unknown)
2013-07-25
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p395kz
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