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2013 | 60 | 3 | 387-393
Article title

An organic solvent and surfactant stable α-amylase from soybean seeds

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EN
Abstracts
EN
An organic solvent and surfactant stable α-amylase was obtained from soybean seeds. The direct and indirect effect of various organic solvents (non-polar, polar protic, and polar aprotic) and surfactants on the activity and stability of free enzyme was determined. The enzyme showed a very high catalytic efficiency and stabilization against most of the organic solvents and surfactants tested, except for few. Those organic solvents and surfactants (like chloroform, dimethyl formamide, n-butanol, and Tween 20), which caused an inhibition in enzyme activity, were used to study their effects on immobilized enzyme. The inhibitory effect was found to be decreased in immobilized enzyme as compared to free enzyme indicating that immobilization imparted stability to the enzyme. Moreover, the possibility of reuse of the enzyme in the presence of the organic solvents and surfactants was increased upon immobilization. The stability of soybean α-amylase towards organic solvents and surfactants shows that it is a potential candidate for use in organic-solvent biocatalysis as well as in detergent industries.
Keywords
Publisher

Year
Volume
60
Issue
3
Pages
387-393
Physical description
Dates
published
2013
received
2012-09-25
revised
2013-09-13
accepted
2013-09-19
(unknown)
2013-09-20
Contributors
  • Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi U.P., India
author
  • Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi U.P., India
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p387kz
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