Changes of DNA methylation and hydroxymethylation in plant protoplast cultures

• Authors: Pavla Moricová , Vladan Ondřej , Božena Navrátilová , Lenka Luhová
• Languages of publication: EN

Abstracts

EN

Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.

Keywords

EN

Brassica oleracea; DNA hydroxymethylation; Cucumis sativus; protoplasts; DNA methylation

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2013
• Volume: 60
• Issue: 1
• Pages: 33-36

Dates

published

2013

received

2012-06-21

revised

2013-01-08

accepted

2013-02-12

(unknown)

2013-03-15

Contributors

author

Pavla Moricová

• Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic

author

Vladan Ondřej

• Department of Botany, Faculty of Science, Palacýi University, Olomouc, Czech Republic

author

Božena Navrátilová

• Department of Botany, Faculty of Science, Palacýi University, Olomouc, Czech Republic

author

Lenka Luhová

• Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic

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