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2013 | 60 | 1 | 33-36
Article title

Changes of DNA methylation and hydroxymethylation in plant protoplast cultures

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Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.
Physical description
  • Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic
  • Department of Botany, Faculty of Science, Palacýi University, Olomouc, Czech Republic
  • Department of Botany, Faculty of Science, Palacýi University, Olomouc, Czech Republic
  • Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic
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