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2013 | 60 | 1 | 33-36
Article title

Changes of DNA methylation and hydroxymethylation in plant protoplast cultures

Content
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EN
Abstracts
EN
Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.
Publisher

Year
Volume
60
Issue
1
Pages
33-36
Physical description
Dates
published
2013
received
2012-06-21
revised
2013-01-08
accepted
2013-02-12
(unknown)
2013-03-15
Contributors
  • Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic
  • Department of Botany, Faculty of Science, Palacýi University, Olomouc, Czech Republic
  • Department of Botany, Faculty of Science, Palacýi University, Olomouc, Czech Republic
author
  • Department of Biochemistry, Faculty of Science, Palacýi University, Olomouc, Czech Republic
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p33kz
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