Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

PL EN


Preferences help
enabled [disable] Abstract
Number of results
2013 | 60 | 2 | 255-258

Article title

The antigenic character of zinc-binding proteins and their localization in boar reproductive tract - A preliminary study

Content

Title variants

Languages of publication

EN

Abstracts

EN
In this study immunoelectrophoretic and double immunodiffusion analyses were used to investigate the antigenic character of zinc-binding proteins (ZnBPs), whereas the indirect immunofluorescence technique was used to identify their origin in boar reproductive tract. The mmunoelectrophoretic analysis of ZnBPs of the seminal plasma resulted in the appearance of three antigenic protein complexes, while specific immunoreactivity patterns of the anti-ZnBP serum were detected by double immunodiffusion analysis. Indirect immunofluorescence technique confirmed that ZnBPs were secreted by different reproductive tract tissues, suggesting their contributions to the seminal plasma.

Year

Volume

60

Issue

2

Pages

255-258

Physical description

Dates

published
2013
received
2013-02-08
revised
2013-04-25
accepted
2013-06-05
(unknown)
2013-06-12

Contributors

  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

References

  • Arver S, Eliasson R (1982) Zinc and zinc ligands in human seminal plasma. II. Contribution by ligands of different origin to the zinc binding properties of human seminal plasma. Acta Physiol Scand 115: 217-24.
  • Boursnell JC, Noble EA, Andrews MG (1975) Boar seminal zinc-precipitable protein and the haemagglutinin. J Reprod Fertil 45: 415-420.
  • Henkel R, Baldauf C, Schill WB (2003) Resorption of the element zinc from spermatozoa by the epididymal epithelium. Reprod Domest Anim 38: 97-101.
  • Hołody D, Strzeżek J (1999) Heparin- and Zn2+-binding proteins from boar seminal plasma. Acta Biochim Pol 46: 935-939.
  • Jonáková V, Mañásková P, Tichá M (2007) Separation, characterization and identification of boar seminal plasma proteins. J Chromatogr B 849: 307-314.
  • Mañásková P, Jonáková V. (2008) Localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract and spermatozoa. J Reprod Immunol 78: 40-48.
  • Mogielnicka-Brzozowska M, Fraser L, Czarzasta J, Kordan W (2012) Isolation and characterization of zinc-binding proteins from canine seminal plasma. Polish J Vet Sci 15: 493-498.
  • Mogielnicka-Brzozowska M, Wysocki P, Strzeżek J, Kordan W (2011) Zinc-binding proteins from boar seminal plasma - isolation, biochemical characteristics and influence on spermatozoa stored at 4°C. Acta Biochim Pol 58: 171-177.
  • Ouchterlony O (1963) Gel-diffusion techniques. 55 'Immunological methods'. Ackroyd JF eds, Bracewell Sci., Publ. Oxford.
  • Rodriguez-Martinez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ (2011) Seminal plasma proteins: What role do they play? Am J Reprod Immunol 66: 11-22.
  • Scheidegger JJ (1955) Une micro-metode de l'immuno-elecrtrophorese, (A micro-method of immunoelectrophoresis). Int Arch Allergy Appl Immunol 7: 103.
  • Strzeżek J, Hopfer E, Zaborniak A (1987) Zinc ion dependent protein in boar semen, II. Effects on sperm motility and antibacterial properties, Anim Reprod Sci 13: 133-142.
  • Strzeżek J, Hopfer E (1987) Zinc ion-dependent protein in boar semen, I. Egg yolk precipitating activity and some biochemical proteins. Anim Reprod Sci 13: 117-31.
  • Veselsky L, Dostal J, Kraus M, Peknicova J, Holan V, Zajicova A, Jonáková V, Zelezna B (2002) Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction. Anim Reprod Sci 71: 111-123.
  • Vivacqua A, Siciliano L, Sabato M, Palma A, Carpino A (2004) Prostasomes as zinc ligands in human seminal plasma. Int J Androl 27: 27-31.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.bwnjournal-article-abpv60p255kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.