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2013 | 60 | 2 | 255-258
Article title

The antigenic character of zinc-binding proteins and their localization in boar reproductive tract - A preliminary study

Content
Title variants
Languages of publication
EN
Abstracts
EN
In this study immunoelectrophoretic and double immunodiffusion analyses were used to investigate the antigenic character of zinc-binding proteins (ZnBPs), whereas the indirect immunofluorescence technique was used to identify their origin in boar reproductive tract. The mmunoelectrophoretic analysis of ZnBPs of the seminal plasma resulted in the appearance of three antigenic protein complexes, while specific immunoreactivity patterns of the anti-ZnBP serum were detected by double immunodiffusion analysis. Indirect immunofluorescence technique confirmed that ZnBPs were secreted by different reproductive tract tissues, suggesting their contributions to the seminal plasma.
Year
Volume
60
Issue
2
Pages
255-258
Physical description
Dates
published
2013
received
2013-02-08
revised
2013-04-25
accepted
2013-06-05
(unknown)
2013-06-12
Contributors
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
References
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  • Boursnell JC, Noble EA, Andrews MG (1975) Boar seminal zinc-precipitable protein and the haemagglutinin. J Reprod Fertil 45: 415-420.
  • Henkel R, Baldauf C, Schill WB (2003) Resorption of the element zinc from spermatozoa by the epididymal epithelium. Reprod Domest Anim 38: 97-101.
  • Hołody D, Strzeżek J (1999) Heparin- and Zn2+-binding proteins from boar seminal plasma. Acta Biochim Pol 46: 935-939.
  • Jonáková V, Mañásková P, Tichá M (2007) Separation, characterization and identification of boar seminal plasma proteins. J Chromatogr B 849: 307-314.
  • Mañásková P, Jonáková V. (2008) Localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract and spermatozoa. J Reprod Immunol 78: 40-48.
  • Mogielnicka-Brzozowska M, Fraser L, Czarzasta J, Kordan W (2012) Isolation and characterization of zinc-binding proteins from canine seminal plasma. Polish J Vet Sci 15: 493-498.
  • Mogielnicka-Brzozowska M, Wysocki P, Strzeżek J, Kordan W (2011) Zinc-binding proteins from boar seminal plasma - isolation, biochemical characteristics and influence on spermatozoa stored at 4°C. Acta Biochim Pol 58: 171-177.
  • Ouchterlony O (1963) Gel-diffusion techniques. 55 'Immunological methods'. Ackroyd JF eds, Bracewell Sci., Publ. Oxford.
  • Rodriguez-Martinez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ (2011) Seminal plasma proteins: What role do they play? Am J Reprod Immunol 66: 11-22.
  • Scheidegger JJ (1955) Une micro-metode de l'immuno-elecrtrophorese, (A micro-method of immunoelectrophoresis). Int Arch Allergy Appl Immunol 7: 103.
  • Strzeżek J, Hopfer E, Zaborniak A (1987) Zinc ion dependent protein in boar semen, II. Effects on sperm motility and antibacterial properties, Anim Reprod Sci 13: 133-142.
  • Strzeżek J, Hopfer E (1987) Zinc ion-dependent protein in boar semen, I. Egg yolk precipitating activity and some biochemical proteins. Anim Reprod Sci 13: 117-31.
  • Veselsky L, Dostal J, Kraus M, Peknicova J, Holan V, Zajicova A, Jonáková V, Zelezna B (2002) Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction. Anim Reprod Sci 71: 111-123.
  • Vivacqua A, Siciliano L, Sabato M, Palma A, Carpino A (2004) Prostasomes as zinc ligands in human seminal plasma. Int J Androl 27: 27-31.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p255kz
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