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2013 | 60 | 1 | 21-31
Article title

Cytoprotective effect of methanolic extract of Nardostachys jatamansi against hydrogen peroxide induced oxidative damage in C6 glioma cells

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Abstracts
EN
Oxidative stress has been implicated as an important factor in the process of neurodegeneration and hydrogen peroxide (H2O2) is one of the most important precursors of reactive oxygen species (ROS), responsible for many neurodegenerative diseases. This study used extracts from Nardostachys jatamansi rhizomes, known for nerve relaxing properties in Ayurvedic medicine, to ascertain their protective role in H2O2-induced oxidative stress in C6 glioma cells. The protective effect of methanolic, ethanolic and water extracts of N. jatamansi (NJ-MEx, NJ-EEx and NJ-WEx respectively) was determined by MTT assay. NJ-MEx significantly protected against H2O2 cytotoxicity when cells were pretreated for 24 h. The level of antioxidant enzymes, catalase, superoxide dismutase (Cu-ZnSOD), glutathione peroxidase (GPx), and a direct scavenger of free radicals, glutathione (GSH), significantly increased following pre-treatment with NJ-MEx. Lipid peroxidation (LPx) significantly decreased in NJ-MEx-pretreated cultures. The expression of a C6 differentiation marker, GFAP (glial fibrillary acidic protein), stress markers HSP70 (heat shock protein) and mortalin (also called glucose regulated protein 75, Grp75) significantly decreased when cells were pre-treated with NJ-MEx before being subjected to H2O2 treatment as shown by immunofluorescence, western blotting and RT-PCR results. The present study suggests that NJ-MEx could serve as a potential treatment and/or preventive measure against neurodegenerative diseases.
Publisher

Year
Volume
60
Issue
1
Pages
21-31
Physical description
Dates
published
2013
received
2012-05-10
revised
2012-11-12
accepted
2013-02-21
(unknown)
2013-03-20
Contributors
  • Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar- Punjab, India
author
  • Department of Biotechnology, DAV College, Amritsar, Punjab, India
author
  • Department of Biotechnology, DAV College, Amritsar, Punjab, India
  • Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar- Punjab, India
  • Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar- Punjab, India
References
  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105: 121-126.
  • Ahmad M, Yousuf S, Khan MB, Hoda MN, Ahmad AS, Ansari MA, Ishrat T, Agrawal AK, Islam F (2006) Attenuation by Nardostachys jatamansi of 6-hydroxydopamine-induced parkinsonism in rats: behavioral, neurochemical, and immunohistochemical studies. Pharmacol Biochem Behav 83: 150-160.
  • Altiok N, Ersoz M, Karpuz V, Koyuturk M (2006) Ginkgo biloba extract regulates differentially the cell death induced by hydrogen peroxide and simvastatin. Neurotoxicology 27: 158-163.
  • Axelsen PH, Komatsu H, Murray IV (2011) Oxidative stress and cell membranes in the pathogenesis of Alzheimer's disease. Physiology (Bethesda) 26: 54-69.
  • Bayir H, Kagan VE, Clark RS, Janesko-Feldman K, Rafikov R, Huang Z, Zhang X, Vagni V, Billiar TR, Kochanek PM (2007) Neuronal NOS-mediated nitration and inactivation of manganese superoxide dismutase in brain after experimental and human brain injury. J Neurochem 101: 168-181.
  • Benda P, Lightbody J, Sato G, Levine L, Sweet W (1968) Differentiated rat glial cell strain in tissue culture. Science 161: 370-371.
  • Bjornstedt M, Xue J, Huang W, Akesson B, Holmgren A (1994) The thioredoxin and glutaredoxin systems are efficient electron donors to human plasma glutathione peroxidase. J Biol Chem 269: 29382-29384.
  • Borniquel S, Valle I, Cadenas S, Lamas S, Monsalve M (2006) Nitric oxide regulates mitochondrial oxidative stress protection via the transcriptional coactivator PGC-1alpha. FASEB J 20: 1889-1891.
  • Brenner S, Gulden M, Maser E, Seibert H (2010) Lasting effect of preceding culture conditions on the susceptibility of C6 cells to peroxide-induced oxidative stress. Toxicol In Vitro 24: 2090-2096.
  • Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol 52: 302-310.
  • Calabrese V, Scapagnini G, Giuffrida Stella AM, Bates TE, Clark JB (2001) Mitochondrial involvement in brain function and dysfunction: relevance to aging, neurodegenerative disorders and longevity. Neurochem Res 26: 739-764.
  • Carette J, Lehnert S, Chow TY (2002) Implication of PBP74/mortalin/GRP75 in the radio-adaptive response. Int J Radiat Biol 78: 183-190.
  • Caviness JN, Lue L, Adler CH, Walker DG (2011) Parkinson's disease dementia and potential therapeutic strategies. CNS Neurosci Ther 17: 32-44.
  • Chatterjee A, Basak B, Saha M, Dutta U, Mukhopadhyay C, Banerji J, Konda Y, Harigaya Y (2000) Structure and stereochemistry of nardostachysin, a new terpenoid ester constituent of the rhizomes of Nardostachys jatamansi. J Nat Prod 63: 1531-1533.
  • Chen CM (2011) Mitochondrial dysfunction, metabolic deficits, and increased oxidative stress in Huntington's disease. Chang Gung Med J 34: 135-152.
  • Chow AM, Brown IR (2007) Induction of heat shock proteins in differentiated human and rodent neurons by celastrol. Cell Stress Chaperones 12: 237-244.
  • Colombrita C, Calabrese V, Stella AM, Mattei F, Alkon DL, Scapagnini G (2003) Regional rat brain distribution of heme oxygenase-1 and manganese superoxide dismutase mRNA: relevance of redox homeostasis in the aging processes. Exp Biol Med (Maywood) 228: 517-524.
  • Doeppner TR, Hermann DM (2010) Free radical scavengers and spin traps - therapeutic implications for ischemic stroke. Best Pract Res Clin Anaesthesiol 24: 511-520.
  • Dringen R, Kussmaul L, Gutterer JM, Hirrlinger J, Hamprecht B (1999) The glutathione system of peroxide detoxification is less efficient in neurons than in astroglial cells. J Neurochem 72: 2523-2530.
  • Eng LF (1985) Glial fibrillary acidic protein (GFAP): the major protein of glial intermediate filaments in differentiated astrocytes. J Neuroimmunol 8: 203-214.
  • Eng LF, Ghirnikar RS (1994) GFAP and astrogliosis. Brain Pathol 4: 229-237.
  • Giffard RG, Xu L, Zhao H, Carrico W, Ouyang Y, Qiao Y, Sapolsky R, Steinberg G, Hu B, Yenari MA (2004) Chaperones, protein aggregation, and brain protection from hypoxic/ischemic injury. J Exp Biol 207: 3213-3220.
  • Gulden M, Jess A, Kammann J, Maser E, Seibert H (2010) Cytotoxic potency of H2O2 in cell cultures: impact of cell concentration and exposure time. Free Radic Biol Med 49: 1298-1305.
  • Hadari YR, Haring HU, Zick Y (1997) p75, a member of the heat shock protein family, undergoes tyrosine phosphorylation in response to oxidative stress. J Biol Chem 272: 657-662.
  • Halliwell B (2012) Free radicals and antioxidants: updating a personal view. Nutr Rev 70: 257-265.
  • Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine. Oxford; New York: Clarendon Press; Oxford University Press.
  • Han D, Sen CK, Roy S, Kobayashi MS, Tritschler HJ, Packer L (1997) Protection against glutamate-induced cytotoxicity in C6 glial cells by thiol antioxidants. Am J Physiol 273: R1771-R1778.
  • Han X, Pan J, Ren D, Cheng Y, Fan P, Lou H (2008) Naringenin-7-O-glucoside protects against doxorubicin-induced toxicity in H9c2 cardiomyocytes by induction of endogenous antioxidant enzymes. Food Chem Toxicol 46: 3140-3146.
  • Hansen MB, Nielsen SE, Berg K (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 119: 203-210.
  • Harborne JB (1998) Phytochemical methods : a guide to modern techniques of plant analysis. London; New York: Chapman and Hall.
  • Hertz L, Dringen R, Schousboe A, Robinson SR (1999) Astrocytes: glutamate producers for neurons. J Neurosci Res 57: 417-428.
  • Katayama S, Ishikawa S, Fan MZ, Mine Y (2007) Oligophosphopeptides derived from egg yolk phosvitin up-regulate gamma-glutamylcysteine synthetase and antioxidant enzymes against oxidative stress in Caco-2 cells. J Agric Food Chem 55: 2829-2835.
  • Kim HS, Lee K, Kang KA, Lee NH, Hyun JW (2012) Phloroglucinol exerts protective effects against oxidative stress-induced cell damage in SH-SY5Y cells. J Pharmacol Sci 119: 186-192.
  • Konar A, Shah N, Singh R, Saxena N, Kaul SC, Wadhwa R, Thakur MK (2011) Protective role of Ashwagandha leaf extract and its component withanone on scopolamine-induced changes in the brain and brain-derived cells. PLoS One 6: e27265.
  • Kono Y (1978) Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys 186: 189-195.
  • Landry CF, Ivy GO, Brown IR (1990) Developmental expression of glial fibrillary acidic protein mRNA in the rat brain analyzed by in situ hybridization. J Neurosci Res 25: 194-203.
  • Liu B, Gao HM, Wang JY, Jeohn GH, Cooper CL, Hong JS (2002) Role of nitric oxide in inflammation-mediated neurodegeneration. Ann N Y Acad Sci 962: 318-331.
  • Lyle N, Bhattacharyya D, Sur TK, Munshi S, Paul S, Chatterjee S, Gomes A (2009) Stress modulating antioxidant effect of Nardostachys jatamansi. Indian J Biochem Biophys 46: 93-98.
  • Maroto R, Perez-Polo JR (1997) BCL-2-related protein expression in apoptosis: oxidative stress versus serum deprivation in PC12 cells. J Neurochem 69: 514-523.
  • Massa SM, Longo FM, Zuo J, Wang S, Chen J, Sharp FR (1995) Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain. J Neurosci Res 40: 807-819.
  • Mitsumoto A, Takeuchi A, Okawa K, Nakagawa Y (2002) A subset of newly synthesized polypeptides in mitochondria from human endothelial cells exposed to hydroperoxide stress. Free Radic Biol Med 32: 22-37.
  • Murphy MP (1999) Nitric oxide and cell death. Biochim Biophys Acta 1411: 401-414.
  • Osorio C, Sullivan PM, He DN, Mace BE, Ervin JF, Strittmatter WJ, Alzate O (2007) Mortalin is regulated by APOE in hippocampus of AD patients and by human APOE in TR mice. Neurobiol Aging 28: 1853-1862.
  • Pekny M, Nilsson M (2005) Astrocyte activation and reactive gliosis. Glia 50: 427-434.
  • Rajdev S, Sharp FR (2000) Stress proteins as molecular markers of neurotoxicity. Toxicol Pathol 28: 105-112.
  • Rasheed AS, Venkataraman S, Jayaveera KN, Fazil AM, Yasodha KJ, Aleem MA, Mohammed M, Khaja Z, Ushasri B, Pradeep HA, Ibrahim M (2010) Evaluation of toxicological and antioxidant potential of Nardostachys jatamansi in reversing haloperidol-induced catalepsy in rats. Int J Gen Med 3: 127-136.
  • Rohrdanz E, Schmuck G, Ohler S, Kahl R (2001) The influence of oxidative stress on catalase and MnSOD gene transcription in astrocytes. Brain Res 900: 128-136.
  • Rutka JT, De Armond SJ, Giblin J, McCulloch JR, Wilson CB, Rosenblum ML (1988) Effect of retinoids on the proliferation, morphology and expression of glial fibrillary acidic protein of an anaplastic astrocytoma cell line. Int J Cancer 42: 419-427.
  • Salim S, Ahmad M, Zafar KS, Ahmad AS, Islam F (2003) Protective effect of Nardostachys jatamansi in rat cerebral ischemia. Pharmacol Biochem Behav 74: 481-486.
  • Sauer H, Wartenberg M, Hescheler J (2001) Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell Physiol Biochem 11: 173-186.
  • Scapagnini G, Ravagna A, Bella R, Colombrita C, Pennisi G, Calvani M, Alkon D, Calabrese V (2002) Long-term ethanol administration enhances age-dependent modulation of redox state in brain and peripheral organs of rat: protection by acetyl carnitine. Int J Tissue React 24: 89-96.
  • Schipke CG, Ohlemeyer C, Matyash M, Nolte C, Kettenmann H, Kirchhoff F (2001) Astrocytes of the mouse neocortex express functional N-methyl-D-aspartate receptors. FASEB J 15: 1270-1272.
  • Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 25: 192-205.
  • Sharma SK, Singh AP (2012) In vitro antioxidant and free radical scavenging activity of Nardostachys jatamansi DC. J Acupunct Meridian Stud 5: 112-118.
  • Simonian NA, Coyle JT (1996) Oxidative stress in neurodegenerative diseases. Annu Rev Pharmacol Toxicol 36: 83-106.
  • Singh J, Kaur G (2009) Transcriptional regulation of PSA-NCAM expression by NMDA receptor activation in RA-differentiated C6 glioma cultures. Brain Res Bull 79: 157-168.
  • Stewart VC, Stone R, Gegg ME, Sharpe MA, Hurst RD, Clark JB, Heales SJ (2002) Preservation of extracellular glutathione by an astrocyte derived factor with properties comparable to extracellular superoxide dismutase. J Neurochem 83: 984-991.
  • Subashini R, Ragavendran B, Gnanapragasam A, Yogeeta SK, Devaki T (2007) Biochemical study on the protective potential of Nardostachys jatamansi extract on lipid profile and lipid metabolizing enzymes in doxorubicin intoxicated rats. Pharmazie 62: 382-387.
  • Taurin S, Seyrantepe V, Orlov SN, Tremblay TL, Thibault P, Bennett MR, Hamet P, Pshezhetsky AV (2002) Proteome analysis and functional expression identify mortalin as an antiapoptotic gene induced by elevation of [Na+]i/[K+]i ratio in cultured vascular smooth muscle cells. Circ Res 91: 915-922.
  • Terashvili M, Sarkar P, Nostrand MV, Falck JR, Harder DR (2012) The protective effect of astrocyte-derived 14,15-epoxyeicosatrienoic acid on hydrogen peroxide-induced cell injury in astrocyte-dopaminergic neuronal cell line co-culture. Neuroscience 223: 68-76.
  • Toda M, Miura M, Asou H, Sugiyama I, Kawase T, Uyemura K (1999) Suppression of glial tumor growth by expression of glial fibrillary acidic protein. Neurochem Res 24: 339-343.
  • Vinutha B, Prashanth D, Salma K, Sreeja SL, Pratiti D, Padmaja R, Radhika S, Amit A, Venkateshwarlu K, Deepak M (2007) Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. J Ethnopharmacol 109: 359-363.
  • Wadhwa R, Kaul SC, Mitsui Y, Sugimoto Y (1993) Differential subcellular distribution of mortalin in mortal and immortal mouse and human fibroblasts. Exp Cell Res 207: 442-448.
  • Wang J, Wang X, Jiang S, Yuan S, Lin P, Zhang J, Lu Y, Wang Q, Xiong Z, Wu Y, Ren J, Yang H (2007) Growth inhibition and induction of apoptosis and differentiation of tanshinone IIA in human glioma cells. J Neurooncol 82: 11-21.
  • Yu HH, Hur JM, Seo SJ, Moon HD, Kim HJ, Park RK, You YO (2009) Protective effect of ursolic acid from Cornus officinalis on the hydrogen peroxide-induced damage of HEI-OC1 auditory cells. Am J Chin Med 37: 735-746.
  • Zhang R, Chae S, Kang KA, Piao MJ, Ko DO, Wang ZH, Park DB, Park JW, You HJ, Hyun JW (2008) Protective effect of butin against hydrogen peroxide-induced apoptosis by scavenging reactive oxygen species and activating antioxidant enzymes. Mol Cell Biochem 318: 33-42.
  • Zhang R, Kang KA, Piao MJ, Kim KC, Kim AD, Chae S, Park JS, Youn UJ, Hyun JW (2010) Cytoprotective effect of the fruits of Lycium chinense Miller against oxidative stress-induced hepatotoxicity. J Ethnopharmacol 130: 299-306.
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bwmeta1.element.bwnjournal-article-abpv60p21kz
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