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2013 | 60 | 1 | 107-110
Article title

Isolation of low-molecular albumins of 2S fraction from soybean (Glycine max (L.) Merrill)

Content
Title variants
Languages of publication
EN
Abstracts
EN
Numerous studies have shown that consumption of soybean products decrease the risk of cancers in humans. Experiments at the molecular level have demonstrated that in most cases proteins and peptides are responsible for the anticancer properties of soybeen. Special attention should be paid to lunasin - a peptide described for the first time 16 years ago. Due to its structure it causes i.a., inhibition of cancer cell proliferation. A novel procedure for the isolation and purification of low-molecular-mass 2S soybean albumin protein is described in the present paper. A fraction of four peptides one of them corresponding to molecular mass and isoelectric point characteristic for lunasin. The obtained peptides decreased on the rate of HeLa cell proliferation.
Keywords
Publisher

Year
Volume
60
Issue
1
Pages
107-110
Physical description
Dates
published
2013
received
2012-12-13
revised
2013-02-18
accepted
2013-03-14
(unknown)
2013-03-21
Contributors
  • Department of Biochemistry and Biotechnology, Faculty of Agronomy and Bioengineering, University of Life Sciences, Poznań, Poland
  • Department of Biochemistry and Biotechnology, Faculty of Agronomy and Bioengineering, University of Life Sciences, Poznań, Poland
author
  • NanoBiomedical Centre, Adam Mickiewicz University, Poznań, Poland
  • Department of Biochemistry and Biotechnology, Faculty of Agronomy and Bioengineering, University of Life Sciences, Poznań, Poland
author
  • Department of General and Environmental Microbiology, Faculty of Agronomy and Bioengineering, University of Life Sciences, Poznań, Poland
References
  • Lin J, Shewry PR, Archer DB, Beyer K, Niggemann B, Haas H, Wilson P, Alcocer MJ (2006) The potential allergenicity of two 2S albumins from soybean (Glycine max): a protein microarray approach, Int Arch Allergy Immunol 141: 91-102.
  • L'hocine L, Boye JI, Arcand Y (2006) Composition and functional properties of soy protein isolates prepared using alternative defatting and extraction procedures. J Food Sci 71: 137-145.
  • Seber LE, Barnett B, McConnell W, Hume SD, Cai JB, Davis KR (2012) Scalable purification and characterization of the anticancer lunasin peptide from soybean. PLoS ONE 7: e35409.
  • Galvez AF, de Lumen BO (1999) A soybean cDNA encoding a chromatin binding peptide inhibits mitosis of mammalian cells. Nat Biotechnol 17: 495-500.
  • Hernández-Ledesma B and de Lumen BO (2008) Lunasin: a novel cancer preventive seed peptide. Perspect Medicin Chem 2: 75-80.
  • Hsieh C, Hernández-Ledesma B and de Lumen BO (2011) Lunasin, a new breast cancer chemopreventive seed peptide. Breast Cancer - Current and Alternative Therapeutic Modalities 11: 215-242.
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  • Galvez AF, Chen N, Macasieb J, de Lumen BO (2001) Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res 61: 7473-7478.
  • Gonzalez E (2004) Lunasin concentration in different soybean genotypes, commercial soy protein, and isoflavone products J Agric Food Chem 52: 5882-5887.
  • Liu KS (1997) Chemistry and nurtitional value of soybean components. In Soybean Chemistry Technology and Utilization, pp 25-113. Chapman and Hall, New York.
  • Czarnecka J, Koziołkiewicz M (2007) Albuminy 2S - roślinne białka zapasowe o właściwościach alergennych. Biotechnologia 2: 114-127 (in Polish).
  • Galvez AF (2010) Products and methods using soy peptides to lower total and LDL cholesterol levels. Patent application publication. Pub. No 20100087367 (West Sacramento, CA, US).
  • Wang W, Mcjia EG (2005) Soybean Association and the USDA Functional Foods. Comp Rev Food Sd Food Sate 4: 63-78.
  • Amnuaycheewa P, Gonzalez de Mejia E (2010) Purification, Characterisation, and quantification of the soy allergen profilin (Gly m 3) in soy products. Food Chem 119: 1671-1680.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv60p107kz
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