Polarization angle dependence of stark absorption spectra of spirilloxanthin bound to the reconstituted LH1 complexes using LH1-subunits isolated from the purple photosynthetic bacterium Rhodospirillum rubrum

• Authors: Tomoko Horibe , Katsunori Nakagawa , Toshiyuki Kusumoto , Ritsuko Fujii , Richard J. Cogdell , Mamoru Nango , Hideki Hashimoto
• Languages of publication: EN

Abstracts

EN

Reconstituted LH1 complexes were prepared using the LH1 subunit-type complexes, isolated from the purple photosynthetic bacterium Rhodospirillum (Rs.) rubrum, and purified all-trans spirilloxanthin. Stark absorption spectra of spirilloxanthin bound to both the native and reconstituted LH1 complexes were compared in different polarization angles (χ) against the external electric field. From the polarization angle dependence of the Stark absorption spectra, two angles were determined in reference to the direction of transition dipole moment (m) of spirilloxanthin: one is the change in polarizability upon photoexcitation (Δα), θΔα and the other is the change in static dipole moment upon photoexcitation (Δμ), θΔμ. Despite the symmetric molecular structure of all-trans spirilloxanthin, its Stark absorption spectra show pronounced values of Δμ. This large Δμ values essentially caused by the effect of induced dipole moment through Δα both in the cases for native and reconstituted LH1 complexes. However, slightly different values of θΔα and θΔμ observed for the native LH1 complex suggest that spirilloxanthin is asymmetrically distorted when bound to the native LH1 complex and gives rise to intrinsic Δμ value.

Keywords

EN

spirilloxanthin; Rhodospirillum rubrum; Stark absorption spectroscopy; polarization angle dependence; reconstitution; carotenoid; LH1 complex

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 1
• Pages: 97-100

Dates

published

2012

received

2011-10-14

accepted

2012-03-01

(unknown)

2012-03-17

Contributors

author

Tomoko Horibe

• Department of Physics, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan

author

Katsunori Nakagawa

• CREST/JST, Honcho Kawaguchi, Saitama, Japan

author

Toshiyuki Kusumoto

• Department of Physics, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan

author

Ritsuko Fujii

• CREST/JST, Honcho Kawaguchi, Saitama, Japan

author

Richard J. Cogdell

• Department of Life and Materials Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Japan

author

Mamoru Nango

• CREST/JST, Honcho Kawaguchi, Saitama, Japan

author

Hideki Hashimoto

• Department of Physics, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan

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