Polarization angle dependence of stark absorption spectra of spirilloxanthin bound to the reconstituted LH1 complexes using LH1-subunits isolated from the purple photosynthetic bacterium Rhodospirillum rubrum
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Reconstituted LH1 complexes were prepared using the LH1 subunit-type complexes, isolated from the purple photosynthetic bacterium Rhodospirillum (Rs.) rubrum, and purified all-trans spirilloxanthin. Stark absorption spectra of spirilloxanthin bound to both the native and reconstituted LH1 complexes were compared in different polarization angles (χ) against the external electric field. From the polarization angle dependence of the Stark absorption spectra, two angles were determined in reference to the direction of transition dipole moment (m) of spirilloxanthin: one is the change in polarizability upon photoexcitation (Δα), θΔα and the other is the change in static dipole moment upon photoexcitation (Δμ), θΔμ. Despite the symmetric molecular structure of all-trans spirilloxanthin, its Stark absorption spectra show pronounced values of Δμ. This large Δμ values essentially caused by the effect of induced dipole moment through Δα both in the cases for native and reconstituted LH1 complexes. However, slightly different values of θΔα and θΔμ observed for the native LH1 complex suggest that spirilloxanthin is asymmetrically distorted when bound to the native LH1 complex and gives rise to intrinsic Δμ value.
- Akahane J, Rondonuwu FS, Fiedor L, Watanabe Y, Koyama Y (2004) Dependence of singlet-energy transfer on the conjugation length of carotenoids reconstituted into the LH1 complex from Rhodospirillum rubrum G9. Chem Phys Lett 393: 184-191.
- Bublitz GU, Boxer SG (1997a) Stark spectroscopy: Applications in chemistry, biology, and materials science. Annu Rev Phys Chem 48: 213-242.
- Bublitz GU, Ortiz R, Marder SR, Boxer SG (1997b) Stark spectroscopy of donor/acceptor substituted polyenes. J Am Chem Soc 119: 3365-3376.
- Cogdell RJ, Gall A, Köhler J (2006) The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes. Q Rev Biophys 39: 227-324.
- Cogdell RJ, Gardiner AT, Gabrielsen M, Southall J, Roszak AW, Isaacs NW, Fujii R, Hashimoto H (2008) The structure of purple bacterial antenna complexes. In Photosynthetic Protein Complexes: A Structural Approach, Fromme P ed, pp 325-340. Wiley-Blackwell, Weinheim, Germany.
- Gottfried DS, Steffen MA, Boxer SG (1991) Large protein-induced dipoles for a symmetrical carotenoid in a photosynthetic antenna complex. Science 251: 662-665.
- Iida K, Inagaki JI, Shinohara M, Suemori Y, Ogawa M, Dewa T, Nango M (2005) Near-IR absorption and fluorescence spectra and AFM observation of the light-harvesting 1 complex on a mica substrate refolded from the subunit light-harvesting 1 complexes of photosynthetic bacteria Rhodospirillum rubrum. Langmuir 21: 3069-3075.
- Ke B (2001) Photosynthesis: Photobiochemistry and Photobiophysics, pp 1-46. Kluwer Academic Publishers, Dordrecht, The Netherlands.
- Krawczyk BJ, Luchowski R, Wiącek D (2006) Electroabsorption spectra of carotenoid isomers: Conformational modulation of polarizability vs. induced dipole moments. Chem Phys 326: 465-470.
- Krawczyk S, Daniluk A (1995) Solvent effects and vibrational dependence in electrochromic spectra of carotenoids. Chem Phys Lett 236: 431-437.
- Liptay W (1974) Dipole moments and polarizabilities of molecules in excited electronic states. In Excited States. Lim EC ed, vol. 1, pp 129-229. Academic Press, New York, USA
- Middendorf TR, Mazzola LT, Lao KQ, Steffen MA, Boxer SG (1993) Stark-effect (electroabsorption) spectroscopy of photosynthetic reaction centers at 1.5 K - evidence that the special pair has a large excited-state polarizability. Biochim Biophys Acta 1143: 223-234.
- Nakagawa K, Nakano T, Fukui N, Nakashima A, Sakai A, Mizuno T, Dewa T, Iida K, Gardiner AT, Cogdell RJ, Fujii R, Hashimoto H, Nango M (2009) Reconstitution of the light-harvesting 1 (LH1) Complex Using LH1-a and LH1-b polypeptides, separately isolated from the purple photosynthetic bacterium Rhodospirillum rubrum, together with bacteriochlorophyll a and all-trans carotenoids. Carotenoid Science 14: 54-57.
- Nakagawa K, Suzuki S, Fujii R, Gardiner AT, Cogdell RJ, Nango M, Hashimoto H (2008a) Probing the effect of the binding site on the electrostatic behavior of a series of carotenoids reconstituted into the light-harvesting 1 complex from purple photosynthetic bacterium Rhodospirillum rubrum detected by stark spectroscopy. J Phys Chem B 112: 3467-9475.
- Nakagawa K, Suzuki S, Fujii R, Gardiner AT, Cogdell RJ, Nango M, Hashimoto H (2008b) Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Photosynth Res 95: 339-344.
- Roszak AW, Howard TD, Southall J, Gardiner AT, Law CJ, Isaacs NW, Cogdell RJ (2003) Crystal structure of the RC-LH1 core complex from Rhodopseudomonas palustris. Science 302: 1969-1972.
- Yanagi K, Hashimoto H, Gardiner AT, Cogdell RJ (2004) Stark spectroscopy on the LH2 complex from Rhodobacter sphaeroides strain G1C; frequency and temperature dependence. J Phys Chem B 108: 10334-10339.
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