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2012 | 59 | 1 | 87-90
Article title

EPR study of thylakoid membrane dynamics in mutants of the carotenoid biosynthesis pathway of Synechocystis sp. PCC6803

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EN
Abstracts
EN
EPR spectroscopy using 5-doxylstearic acid (5-SASL) and 16-doxylstearic acid (16-SASL) spin probes was used to study the fluidity of thylakoid membranes. These were isolated from wild type Synechocystis and from several mutants in genes encoding selected enzymes of the carotenoid biosynthesis pathway and/or acyl-lipid desaturases. Cyanobacteria were cultivated at 25°C and 35°C under different light regimes: photoautotrophically (PAG) and/or in light-activated heterotrophic conditions (LAHG). The relative fluidity of membranes was estimated from EPR spectra based on the empirical outermost splitting parameter in a temperature range from 15°C to 40°C. Our findings demonstrate that in native thylakoid membranes the elimination of xanthophylls decreased fluidity in the inner membrane region under optimal growth conditions (25°C) and increased it under sublethal heat stress (35°C). This indicated that the overall fluidity of native photosynthetic membranes in cyanobacteria may be influenced by the ratio of polar to non-polar carotenoid pools under different environmental conditions.
Year
Volume
59
Issue
1
Pages
87-90
Physical description
Dates
published
2012
received
2011-10-17
accepted
2012-03-01
(unknown)
2012-03-17
References
  • Allen MM (1968) Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 4: 1-4.
  • Anderson SL, McIntosh L (1991) Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process. J Bacteriol 173: 2761-2767.
  • Cogdell RJ, Howard TD, Bittl R, Schlodder E, Geisenheimer I, Lubitz W ( 2000 ) How carotenoids protect bacterial photosynthesis. Philos Trans R Soc B: Biol Sci 355: 1345-1349.
  • Fernández-González B, Sandmann G, Vioque A (1997) A new type of asymmetrically acting β-carotene ketolase is required for the synthesis of echinenone in the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem 272: 9728-9733.
  • Gombos Z, Murata N (1998) Genetic engineering of the unsaturation of membrane glycerolipid: effects on the ability of the photosynthetic machinery to tolerate temperature stress. In Lipids in Photosynthesis: Structure, Function, and Genetics, Siegenthaler P-A, Murata N, eds, pp 249-262. Kluwer Academic Publishers, Dordrecht, The Netherlands.
  • Gombos Z, Wada H, Murata N (1992) Unsaturation of fatty acids in membrane lipids enhances tolerance of the cyanobacterium Synechocystis PCC 6803 to low-temperature photoinhibition. Proc Natl Acad Sci USA 89: 9959-9963.
  • Gombos Z, Wada H, Murata N (1994) The recovery of photosynthesis from low-temperature photoinhibition is accelerated by the unsaturation of membrane lipids: a mechanism of chilling tolerance. Proc Natl Acad Sci USA 91: 8787-8791.
  • Gombos Z, Kanervo E, Tsvetkova N, Sakamoto T, Aro EM, Murata N (1997) Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids. Plant Physiol 115: 551-559.
  • Gruszecki WI, Strzałka K (2005) Carotenoids as modulators of lipid membrane physical properties. Biochim Biophys Acta 1740: 108-115.
  • Komenda J, Barber J (1995) Comparison of psbO and psbH deletion mutants of Synechocystis PCC 6803 indicates that degradation of D1 protein is regulated by the QB site and dependent on protein synthesis. Biochemistry 34: 9625-9631.
  • Kota Z, Horvath LI, Droppa M, Horvath G, Farkas T, Pali T (2002) Protein assembly and heat stability in developing thylakoid membranes during greening. Proc Natl Acad Sci USA 99: 12149-12154.
  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148: 350-382.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
  • Masamoto K, Wada H, Kaneko T, Takaichi S (2001) Identification of the gene required for cis-to-trans carotene isomerization in carotenogenesis of the cyanobacterium Synechocystis sp. PCC6803. Plant Cell Physiol 42: 1398-1402.
  • Schäfer L, Vioque A, Sandmann G (2005) Functional in situ evaluation of photosynthesis-protecting carotenoids in mutants of the cyanobacterium Synechocystis PCC6803. J Photochem Photobiol B-Biol 78: 195-201.
  • Schnarrenberger C, Mohr H (1970) Carotenoid synthesis in mustard seedlings as controlled by phytochrome and inhibitors. Planta 94: 296-307.
  • Sozer Ö, Komenda J, Ughy B, Domonkos I, Laczkó-Dobos H, Malec P, Gombos Z, Kis M (2010) Involvement of carotenoids in the synthesis and assembly of protein subnits of photosynthetic reaction centers of Synechocystis sp. PCC6803. Plant Cell Physiol 51: 823-835.
  • Strzałka K, Gruszecki WI (1994) Effect of beta-carotene on structural and dynamic properties of model phosphatidylcholine membranes: I. An EPR spin label study. Biochim Biophys Acta 1194: 138-142.
  • Strzałka K, Hara-Nishimura I, Nishimura M (1995) Changes in physical properties of vacuolar membrane during transformation of protein bodies into vacuoles in germinating pumpkin seeds. Biochim Biophys Acta 1239: 103-110.
  • Subczyński WK, Hyde JS, Kusumi A (1989) Oxygen permeability of phosphatidylcholine-cholesterol membranes. Proc Natl Acad Sci USA 86: 4474-4478.
  • Subczyński WK, Markowska E, Sielewiesiuk J (1991) Effect of polar carotenoids on the oxygen diffusion-concentration product in lipid bilayer. An EPR spin label study. Biochim Biophys Acta 1068: 68-72.
  • Szabo I, Bergantino E, Giacometti GM (2005) Light and oxygenic photosynthesis: energy dissipation as a protection mechanism against photo-oxidation. EMBO Rep 6: 629 - 634.
  • Tasaka Y, Gombos Z, Nishiyama Y, Mohanty P, Ohba T, Ohki K, Murata N (1996) Targeted mutagenesis of acyl-lipid desaturases in Synechocystis: evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis. EMBO J 15: 6416-6425.
  • Wada H, Gombos Z, Sakamoto T, Murata N (1992) Genetic Manipulation of the Extent of Desaturation of Fatty Acids in Membrane Lipids in the Cyanobacterium Synechocystis PCC6803. Plant Cell Physiol 33: 535-540.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p87kz
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