Spectroscopy analysis for simultaneous determination of lycopene and β-carotene in fungal biomass of Blakeslea trispora

• Authors: Iaroslav M. Soroka , Valeriy G. Narushin , Yuriy D. Turiyansky , Alexey A. Tyurenkov
• Languages of publication: EN

Abstracts

EN

Blakeslea trispora is a good alternative source for producing such carotenoids as lycopene and β-carotene. The objective of this research was to elaborate a method for the simultaneous determination of lycopene and β-carotene in Blakeslea trispora products using a usual UV-vis spectrophotometer. The standard solutions of the mixture of different concentrations of β-carotene and lycopene were measured with the UV-vis method and correlation formula for the extinction coefficients of 1% standard solution of lycopene in the solvent (hexane) and the ratios of the optical densities at the character peaks of 470 and 502 nm was elaborated. This gives a possibility to calculate the concentrations of lycopene and β-carotene in the mixture. The prediction quality of the UV-vis method was sufficient and the obtained results were very close to the ones, being measured with the HPLC technique. The proposed method can be used for both routine industrial work and academic research, providing the rapid analysis for simultaneous measurements of lycopene and β-carotene.

Keywords

EN

UV-vis analysis; β-carotene; lycopene

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 1
• Pages: 65-69

Dates

published

2012

received

2011-09-28

accepted

2012-03-01

(unknown)

2012-03-17

Contributors

author

Iaroslav M. Soroka

• Vitan Group, Vitan Ltd., Dneprovsky, Dnepropetrovsk Region, Ukraine

author

Valeriy G. Narushin

• Vitan Group, Vita-Market Ltd., Zaporozhye, Ukraine

author

Yuriy D. Turiyansky

• Vitan Group, Vitan Ltd., Dneprovsky, Dnepropetrovsk Region, Ukraine

author

Alexey A. Tyurenkov

• Vitan Group, Vitan Ltd., Dneprovsky, Dnepropetrovsk Region, Ukraine

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