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2012 | 59 | 1 | 65-69
Article title

Spectroscopy analysis for simultaneous determination of lycopene and β-carotene in fungal biomass of Blakeslea trispora

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Blakeslea trispora is a good alternative source for producing such carotenoids as lycopene and β-carotene. The objective of this research was to elaborate a method for the simultaneous determination of lycopene and β-carotene in Blakeslea trispora products using a usual UV-vis spectrophotometer. The standard solutions of the mixture of different concentrations of β-carotene and lycopene were measured with the UV-vis method and correlation formula for the extinction coefficients of 1% standard solution of lycopene in the solvent (hexane) and the ratios of the optical densities at the character peaks of 470 and 502 nm was elaborated. This gives a possibility to calculate the concentrations of lycopene and β-carotene in the mixture. The prediction quality of the UV-vis method was sufficient and the obtained results were very close to the ones, being measured with the HPLC technique. The proposed method can be used for both routine industrial work and academic research, providing the rapid analysis for simultaneous measurements of lycopene and β-carotene.

Physical description
  • Vitan Group, Vitan Ltd., Dneprovsky, Dnepropetrovsk Region, Ukraine
  • Vitan Group, Vita-Market Ltd., Zaporozhye, Ukraine
  • Vitan Group, Vitan Ltd., Dneprovsky, Dnepropetrovsk Region, Ukraine
  • Vitan Group, Vitan Ltd., Dneprovsky, Dnepropetrovsk Region, Ukraine
  • Baranska M, Schütze W, Schulz H (2006) Determination of lycopene and β-carotene content in tomato fruits and related products: comparison of FT-Raman, ATR-IR, and NIR spectroscopy. Anal Chem 78: 8456-8461.
  • Britton G, Liaaen-Jensen S, Pfander Carotenoids H (2004) Carotenoids Handbook. Birkhäuser Verlag - Basel; Boston; Berlin.
  • β-carotene from Blakeslea trispora (2003) FNP 52, Add. 11. Prepared at the 61st JECFA.
  • Choudhari SM, Ananthanarayan L, Singhal RS (2008) Use of metabolic stimulators and inhibitors for enhanced production of β-carotene and lycopene by Blakeslea trispora NRRL 2895 and 2896. Bioresour Technol 99: 3166-3173.
  • Cserhati T, Forgacs E, Morais MH, Mota T (2000) Liquid chromatography of natural pigments. Biomed Chromatogr 14: 281-286.
  • Echavarri-Erasun C, Johnson EA (2002) Fungal carotenoids. Appl Mycology Biotech 2: 45-85.
  • Emenhiser C, Simonovic N, Sander LC, Schwartz SJ (1996) Separation of geometrical carotenoid isomers in biological extracts using a polymeric C30 column in reversed-phase liquid chromatography. J Agric Food Chem 44: 3887-3893.
  • Ermakov IV, Ermakova MR, Gellermann W, Lademann J (2004) Noninvasive selective detection of lycopene and β-carotene in human skin using Raman spectroscopy. J Biomed Opt 9: 332-338.
  • Lycopene from Blakeslea trispora (2006) FAO JECFA Monographs 3. Prepared at the 67th JECFA.
  • Mantzouridou F, Roukas T, Kotzekidou P, Liakopoulou M (2002) Optimization of β-carotene production from synthetic medium by Blakeslea trispora: a mathematical modeling. Appl Biochem Biotechnol 101: 153-175.
  • Mantzouridou F, Tsimidou MZ (2007) On the monitoring of carotenogenesis by Blakeslea trispora using HPLC. Food Chem 104: 439-444.
  • Mehta BJ, Cerda-Olmedo E (1995) Mutants of carotene production in Blakeslea trispora. Appl Microbiol Biotechnol 42: 836-838.
  • Mehta BJ, Cerda-Olmedo E (1999) Lycopene cyclization in Blakeslea trispora. Mycoscience 40: 307-310.
  • Mehta BJ, Obraztsova IN, Cerda-Olmedo E (2003) Mutants and intersexual heterokaryons of Blakeslea trispora for production of β-carotene and lycopene. Appl Environ Microbiol 69: 4043-4048.
  • Nardo De T, Shiroma-Kian C, Halim Y, Francis D, Rodrigues-Saona LE (2009) Rapid and simultaneous determination of lycopene and β-carotene contents in tomato juice by infrared spectroscopy. J Agric Food Chem 57: 1105-1112.
  • Oliver J, Palou A (2000) Chromatographic determination of carotenoids in foods. J Chromatogr 881: 543-555.
  • Patent of Ukraine No. 83420 (2008) Method of manufacturing of lycopene biomass. Filed Oct. 4, 2006; Publ. Jul. 10, 2008, Bull. No. 13. (in Ukrainian)
  • Pedro AMK, Ferreira MMC (2005) Nondestructive determination of solids and carotenoids in tomato products by near-infrared spectroscopy and multivariate calibration. Anal Chem 77: 2505-2511.
  • Pegklidou K, Mantzouridou F, Tsimidou MZ (2008) Lycopene production using Blakeslea trispora in the presence of 2-methyl imidazole: yield, selectivity, and safety aspects. J Agric Food Chem 56: 4482-4490.
  • Peshkova VM, Gromova MI (1965) Practical Guide on Spectrophotometry and Colorimetry. 2nd edition. Publisher: Moscow University (in Russian).
  • STATISTICA@'99 (1999) Edition. Copyright@ 1984-1999 by StatSoft, Inc.
  • Xu F, Yuan QP, Dong HR (2006) Determination of lycopene and β-carotene by high-performance liquid chromatography using sudan I as internal standard. J Chromatogr B Analyt Technol Biomed Life Sci 838: 44-49.
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