Application of resonance raman microscopy to in vivo carotenoid

• Authors: Chiasa Uragami , Eiji Yamashita , Andrew Gall , Bruno Robert , Hideki Hashimoto
• Languages of publication: EN

Abstracts

EN

The high antioxidant activity of astaxanthin has been attracted considerable attention in these days. One of the major antioxidant activities of this carotenoid is anti-photoaging. We have been focusing our attention on this particular issue. The anti-photoaging activity should be functioning in inner skin. In this study we tried to find out the fact that astaxanthin that has been swabbed on the outer surface of the skin has really passed through and reached to the inner skin. For this purpose resonance Raman microscopy was applied to the rat skin sample on which astaxanthin was swabbed on its outer surface. Astaxanthin gives rise to a unique Raman spectrum that is characteristic of its molecular structure. Therefore, we can easily identify the presence or absence of astaxanthin in the area of the rat skin that is subjected to this spectroscopic measurement. We used 532 nm laser light for probing the resonance Raman scattering of astaxanthin. Astaxanthin shows three strong Raman lines at 1508, 1145, and 993 cm-1. These three lines are ascribable to the C=C stretching, C-C stretching, and C-CH3 in-plane rocking vibrational modes, respectively. We have constructed confocal Raman microscope that has the spatial resolution of ca. 500 nm. Three-dimensional mapping of the Raman spectrum of astaxanthin has been performed in order to determine its distribution in the rat skin.

Keywords

EN

Raman mapping; resonance Raman microscopy; Astaxanthin; in vivo carotenoid

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 1
• Pages: 53-56

Dates

published

2012

received

2011-10-14

accepted

2012-03-01

(unknown)

2012-03-17

Contributors

author

Chiasa Uragami

• Department of Physics and CREST/JST, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan

author

Eiji Yamashita

• Fuji Chemical Industry Co. Ltd., Nakaniikawa-gun, Toyama, Japan

author

Andrew Gall

• Institute of Biology and Technology of Saclay, CEA-Saclay, Gif/Yvette, France

author

Bruno Robert

• Institute of Biology and Technology of Saclay, CEA-Saclay, Gif/Yvette, France

author

Hideki Hashimoto

• Department of Physics and CREST/JST, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan

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