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2012 | 59 | 4 | 537-541
Article title

Stigmasterol blocks cartilage degradation in rabbit model of osteoarthritis

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EN
Abstracts
EN
Stigmasterol has been shown exihbit anti-osteoarthritic properties in vitro studies. However, the in vivo effects of stigmasterol on cartilage are still unclear. This study investigated the anti-osteoarthritic properties of stigmasterol on cartilage degradation in a rabbit model of osteoarthritis (OA). Twenty rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to induce OA. Five rabbits were used as normal control. Two weeks after operation, the rabbits were randomly divided into two groups. Each group of 10 rabbits received intra-articular injection with 0.3 ml of stigmasterol in left knees and vehicle in right knees, once weekly. Group 1 was killed 6 weeks after ACLT and 2 were sacrificed 9 weeks after ACLT. The knee joints were assessed by gross morphology, histology and gene expression analysis. We found that expression of genes encoding matrix metalloproteinases (MMPs) was significantly higher while tissue inhibitors of metalloproteinase (TIMP)-1 was significantly lower in the both joints of the two OA groups compared to normal contrals. Stigmasterol reduced the cartilage degradation as assessed by histological analysis and markedly suppressed MMPs expression both in group 1 and group 2. Our results suggest that stigmasterol may be considered as a possible therapeutical agent in the treatment of OA.
Year
Volume
59
Issue
4
Pages
537-541
Physical description
Dates
published
2012
received
2011-08-11
revised
2012-06-04
accepted
2012-08-20
(unknown)
2012-10-16
References
  • Aigner T, Zien A, Gehrsitz A, Gebhard PM, McKenna L (2001) Anabolic and catabolic gene expression pattern analysis in normal versus osteoarthritic cartilage using complementary DNA-array technology. Arthritis Rheum 44: 2777-2789.
  • Bao JP, Chen WP, Feng J, Zhao J, Shi ZL, Huang K, Wu LD (2009) Variation patterns of two degradation enzyme systems in articular cartilage in different stages of osteoarthritis: regulation by dehydroepiandrosterone. Clin Chim Acta 408: 1-7.
  • Baragi VM, Becher G, Bendele AM, Biesinger R, Bluhm H, Boer J, Deng H, Dodd R, Essers M, Feuerstein T, Gallagher BM Jr, Gege C, Hochgürtel M, Hofmann M, Jaworski A, Jin L, Kiely A, Korniski B, Kroth H, Nix D, Nolte B, Piecha D, Powers TS, Richter F, Schneider M, Steeneck C, Sucholeiki I, Taveras A, Timmermann A, Van Veldhuizen J, Weik J, Wu X, Xia B (2009) A new class of potent matrix metalloproteinase 13 inhibitors for potential treatment of osteoarthritis: Evidence of histologic and clinical efficacy without musculoskeletal toxicity in rat models. Arthritis Rheum 60: 2008-2018.
  • Billinghurst RC, Dahlberg L, Ionescu M, Reiner A, Bourne R, Rorabeck C, Mitchell P, Hambor J, Diekmann O, Tschesche H, Chen J, Van Wart H, Poole AR (1997) Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage. J Clin Invest 99: 1534-1545.
  • Boileau C, Martel-Pelletier J, Caron J, Msika P, Guillou GB, Baudouin C, Pelletier JP (2009) Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13. Arthritis Res Ther 11: R41.
  • Bouic PJ, Etsebeth S, Liebenberg RW, Albrecht CF, Pegel K, Van Jaarsveld PP (1996) beta-Sitosterol and beta-sitosterol glucoside stimulate human peripheral blood lymphocyte proliferation: implications for their use as an immunomodulatory vitamin combination. Int J Immunopharmacol 18: 693-700.
  • Bouic PJ, Lamprecht JH (1999) Plant sterols and sterolins: a review of their immune-modulating properties. Altern Med Rev 4: 170-177.
  • Dean DD, Martel-Pelletier J, Pelletier JP, Howell DS, Woessner JF Jr (1989) Evidence for metalloproteinase and metalloproteinase inhibitor imbalance in human osteoarthritic cartilage. J Clin Invest 84: 678-685.
  • Farkouh ME, Kirshner H, Harrington RA, Ruland S, Verheugt FW, Schnitzer TJ, Burmester GR, Mysler E, Hochberg MC, Doherty M, Ehrsam E, Gitton X, Krammer G, Mellein B, Gimona A, Matchaba P, Hawkey CJ, Chesebro JH, TARGET Study group (2004) Comparison of lumiracoxib with naprox en and ibuprofen in the therapeutic arthritis research and gastrointestinal event trial (TARGET), cardiovascular outcomes: randomised controlled trial. Lancet 364: 675-684.
  • Gabay O, Sanchez C, Salvat C, Chevy F, Breton M, Nourissat G, Wolf C, Jacques C, Berenbaum F (2010) Stigmasterol: a phytosterol with potential anti-osteoarthritic properties. Osteoarthritis Cartilage 18: 106-116.
  • Grether-Beck S, Muhlberg K, Brenden H, Krutmann J (2008) Topical application of vitamins, phytosterols and ceramides. Protection against increased expression of interstital collagenase and reduced collagen-I expression after single exposure to UVA irradiation. Hautarzt 59: 557-562.
  • Gupta MB, Nath R, Srivastava N, Shanker K, Kishor K, Bhargava KP (1980) Anti-inflammatory and antipyretic activities of beta-sitosterol. Planta Med 39: 157-63.
  • Johnson AR, Pavlovsky AG, Ortwine DF, Prior F, Man CF, Bornemeier DA, Banotai CA, Mueller WT, McConnell P, Yan C, Baragi V, Lesch C, Roark WH, Wilson M, Datta K, Guzman R, Han HK, Dyer RD (2007) Discovery and characterization of a novel inhibitor of matrix metalloprotease-13 that reduces cartilage damage in vivo without joint fibroplasia side effects. J Biol Chem 282: 27781-2791.
  • Konttinen YT, Mandelin J, Li TF, Salo J, Lassus J, Liljestrom M, Hukkanen M, Takagi M, Virtanen I, Santavirta S (2002) Acidic cysteine endoproteinase cathepsin K in the degeneration of the superficial articular hyaline cartilage in osteoarthritis. Arthritis Rheum 46: 953-960.
  • Lazzaroni M, Bianchi Porro G (2004) Gastrointestinal side-effects of traditional non-steroidal anti-inflammatory drugs and new formulations. Aliment Pharmacol Ther 20 (Suppl 2): 48-58.
  • Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW (1997) Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion. Ann Rheum Dis 56: 108-115.
  • Navarro A, De las Heras B, Villar A (2001) Anti-inflammatory and immunomodulating properties of a sterol fraction from Sideritis foetens Clemi. Biol Pharm Bull 24: 470-473.
  • Okoye FB, Osadebe PO, Proksch P, Edrada-Ebel RA, Nworu CS, Esimone CO (2010) Anti-inflammatory and membrane-stabilizing stigmastane steroids from Alchornea floribunda leaves. Planta Med 76: 172-177.
  • Pritzker KP, Gay S, Jimenez SA, Ostergaard K, Pelletier JP, Revell PA, Salter D, van den Berg WB (2006) Osteoarthritis cartilage histopathology: grading and staging. Osteoarthritis Cartilage 14: 13-29.
  • Raicht RF, Cohen BI, Fazzini EP, Sarwal AN, Takahashi M (1980) Protective effect of plant sterols against chemically induced colon tumors in rats. Cancer Res 40: 403-405.
  • Rowan AD, Litherland GJ, Hui W, Milner JM (2008) Metalloproteases as potential therapeutic targets in arthritis treatment. Expert Opin Ther Targets 12: 1-18.
  • Thiers H (1953) The value of stigmasterol (R. Wulzen's antistiffness factor essential for guinea pigs) in the therapy of rheumatic diseases. Rev Rhum Mal Osteoartic 20: 636-643.
  • Tortorella MD, Malfait AM, Deccico C, Arner E (2001) The role of ADAM-TS4 (aggrecanase-1) and ADAM-TS5 (aggrecanase-2) in a model of cartilage degradation. Osteoarthritis Cartilage 9: 539-552.
  • Wu H, Du J, Zheng Q (2008) Expression of MMP-1 in cartilage and synovium of experimentally induced rabbit ACLT traumatic osteoarthritis: immunohistochemical study. Rheumatol Int 29: 31-36.
  • Yan C, Boyd DD (2007) Regulation of matrix metalloproteinase gene expression. J Cell Physiol 211: 19-26.
  • Yoshioka M, Shimizu C, Harwood FL, Coutts RD, Amiel D (1997) The effects of hyaluronan during the development of osteoarthritis. Osteoarthritis Cartilage 5: 251-260.
  • Youssef DT, Ibrahim AK, Khalifa SI, Mesbah MK, Mayer AM, van Soest RW (2010) New anti-inflammatory sterols from the Red Sea sponges Scalarispongia aqabaensis and Callyspongia siphonella. Nat Prod Commun 5: 27-31.
  • Yudoh K, Shishido K, Murayama H, Yano M, Matsubayashi K, Takada H, Masuko K, Kato T, Nishioka K (2007) Water-soluble C60 fullerene prevents degeneration of articular cartilage in osteoarthritis via down-regulation of chondrocyte catabolic activity and inhibition of cartilage degeneration during disease development. Arthritis Rheum 56: 3307-3318.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p537kz
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