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2012 | 59 | 3 | 433-439
Article title

Certain protein transducing agents convert translocated proteins into cell killers

Content
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EN
Abstracts
EN
The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.
Publisher

Year
Volume
59
Issue
3
Pages
433-439
Physical description
Dates
published
2012
received
2012-07-03
revised
2012-08-23
accepted
2012-09-02
(unknown)
2012-09-03
Contributors
  • Institute of Structural Biology, CNRS-CEA-UJF, UMR 5075, Grenoble, France
  • Institute of Structural Biology, CNRS-CEA-UJF, UMR 5075, Grenoble, France
  • TheREx, TIMC-IMAG, UMR5525 CNRS/Université Joseph Fourier Faculté de Médecine, Bat. J Roget, La Tronche, France
  • TheREx, TIMC-IMAG, UMR5525 CNRS/Université Joseph Fourier Faculté de Médecine, Bat. J Roget, La Tronche, France
author
  • TheREx, TIMC-IMAG, UMR5525 CNRS/Université Joseph Fourier Faculté de Médecine, Bat. J Roget, La Tronche, France
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
author
  • Department of Pathology, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
author
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
  • Institute of Structural Biology, CNRS-CEA-UJF, UMR 5075, Grenoble, France
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p433kz
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