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2012 | 59 | 3 | 425-431
Article title

Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli

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EN
Abstracts
EN
A trehalose synthase gene from Deinococcus radiodurans (DSMZ 20539) containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinant trehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography and characterized. The expressed enzyme is a homodimer with molecular mass of 126.9 kDa and exhibits the highest activity of 11.35 U/mg at pH 7.6 and at 30°C. DraTreS activity was almost unchanged after 2 h preincubation at 45°C and pH 7.6, and retained about 56% of maximal value after 8 h incubation at 50°C. The DraTreS was strongly inhibited by Cu2+, Hg2+, Zn2+, Al3+ and 10 mM Tris. The Km value of maltose conversion was 290.7 mM.
Year
Volume
59
Issue
3
Pages
425-431
Physical description
Dates
published
2012
received
2012-05-22
revised
2012-08-23
accepted
2012-09-01
(unknown)
2012-09-18
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p425kz
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