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2012 | 59 | 3 | 417-423
Article title

In vitro production of M. × piperita not containing pulegone and menthofuran

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EN
Abstracts
EN
The essential oils (EOs) and static headspaces (HSs) of in vitro plantlets and callus of Mentha x piperita were characterized by GC-MS analysis. Leaves were used as explants to induce in vitro plant material. The EO yields of the in vitro biomass were much lower (0.1% v/w) than those of the parent plants (2% v/w). Many typical mint volatiles were emitted by the in vitro production, but the callus and in vitro plantelet EOs were characterized by the lack of both pulegone and menthofuran. This was an important difference between in vitro and in vivo plant material as huge amounts of pulegone and menthofuran may jeopardise the safety of mint essential oil. Regarding the other characteristic volatiles, menthone was present in reduced amounts (2%) in the in vitro plantlets and was not detected in the callus, even if it represented the main constituent of the stem and leaf EOs obtained from the cultivated mint (26% leaves; 33% stems). The M. piperita callus was characterized by menthol (9%) and menthone (2%), while the in vitro plantlet EO showed lower amounts of both these compounds in favour of piperitenone oxide (45%). Therefore, the established callus and in vitro plantlets showed peculiar aromatic profiles characterized by the lack of pulegone and menthofuran which have to be monitored in the mint oil for their toxicity.
Year
Volume
59
Issue
3
Pages
417-423
Physical description
Dates
published
2012
received
2012-05-18
revised
2012-07-17
accepted
2012-08-27
(unknown)
2012-09-03
References
  • Alkire BH, Simon JE (1993) Water management for midwestern peppermint (Mentha x piperita L.) growing in highly organic soils, Indiana, USA. Acta Hort 344: 544-556.
  • Aflatuni A (1999) The comparative study of mint species grown in Northern Finland. In Mint Research in Finland, Salo R ed. Symposium of Mint Research, Jokioinen, Agricultural Research Centre of Finland, Serie A 66: 74-81.
  • Aflatuni A, Heikkinen K, Tomperi P, Jolanen J, Laine K (2000) Variation in the extract composition of mints of different origin cultivated in Finland. J Essent Oil Res 12: 462-466.
  • Ansari MA, Vasudevan P, Tandon M, Razdan RK (2000) Larvicidal and mosquito repellent action of peppermint (Mentha piperita) oil. Bioresour Technol 71: 267-271.
  • Banthorpe DV (1996) Mentha species (mints): in vitro culture and production of lower terpenoids and pigments. In Biotechnology in Agriculture and Forestry, Bajaj PS ed, vol. 37, pp 202-225. Medicinal and Aromatic Plants IX. Springer-Verlag, Berlin.
  • Bhat S, Maheshwari P, Kumar S, Kumar A. (2002). Mentha species: in vitro regeneration and genetic transformation. Mol Biol Today 3: 11-23.
  • Bruneton J (1995) Pharmacognosy, Phytochemistry, Medicinal Plants, pp 405-466. Lavoisier Publ. Londres, New York, Paris.
  • Burbott AJ, Loomis WD (1967) Effects of light and temperature on the monoterpenes of peppermint. Plant Physiol 42: 20-28.
  • EMEA/HMPWP/52/04, Draft Position Paper ON the use of herbal medicinal products containing pulegone and menthofuran. EMEA 2004, pp 1-4.
  • European Pharmacopeia (1997) 3rd edn. Strasbourg, France: Council of Europe, 1298-1300.
  • Chalchat JC, Garry RP, Michet A (1997) Variation of chemical composition of essential oil of Mentha piperita L. during the growing time. J Essent Oil Res 9: 463-465.
  • Clark RJ, Menary RC (1979) The importance of harvest data and plant density on the yield and quality of Tasmanian peppermint oil. J Amer Soc Hort Sci 104: 702-706.
  • Clark RJ, Menary RC (1981). Variations in composition of peppermint oi1 in relation to production areas. Econ Bot 35: 59-69.
  • Croteau R, Hooper LC (1978) Metabolism of monoterpenes. Acetylation of (-)-menthol by a soluble enzyme preparation from pepermint (Mentha piperita) leaves. Plant Physiol 61: 737-742.
  • Croteau R (1991) Metabolism of monoterpenes in mint (Mentha) species. Planta Med 57: S10-S14.
  • Diemer F, Jullien F, Faure O, Moja S, Colson M, Matthys-Rochon E, Caissard JC (1998) High efficiency transformation of peppermint (Mentha x piperita L.) with Agrobacterium tumefaciens. P1 Sci 136: 101-108.
  • Dorman HJD, Koşar M, Kahlos K, Holm Y, Hiltunen R (2003) Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J Agric Food Chem 51: 4563-4569.
  • Duke JA, Bogenschultz-Godwin MJ, duCellier J, Duke PAK (2002) In Handbook of Medicinal Herbs, 2nd edn, pp 22. CRC, Press, Boca Raton, FL,.
  • ESCOP (1992) Proposal for a European monograph on the medicinal use of Menthae Piperitae aetheroleum peppermint oil. In Proposal for European Monograph on the Medicinal Use. Vol 3.
  • European Pharmacopeias (1994) 2nd edn European Treaty Series No 50, 405-457. Maissonnueve SA, France.
  • Gershenzon J, McConkey ME, Croteau RB (2000) Regulation of monoterpene accumulation in leaves of peppermint. Plant Physiol 122: 205-213.
  • Hefendehl FW, Underhill EW, von Rudloff E (1967) The biosynthesis of the oxygenated monoterpenes in mint. Phytochemistry 6: 823-835.
  • JECFA, 55th meeting (2009) Scientific Opinion of the Panel on Food Contact Material, Enzymes, Flavourings & Processing Aids on a request from the Commission on Flavouring Group Evaluation.
  • Kim T, Kim TY, Bae GW, Lee HJ, Chae YA, Chung I.S. (1996) Improved production of essential oils by two-phase culture of Mentha piperita cells. Plant Tissue Cult Lett 13: 189-192.
  • Hendriks H (1998) Pharmaceutical aspects of some Mentha herbs and their essential oils. Perfumer & Flavorist 23: 15-23.
  • Krasnyanski S, May RA, Loskutov A, Ball TM, Sink KC (1999) Transformation of the limonane synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants. Theor Appl Genet 99: 676-682.
  • Kumar S, Bahl JR, Bansal RP, Kukreja AK, Garg SN, Naqvi AA, Luthra R, Sharma S (2000) Profits of Indian menthol mint Mentha arvensis cultivars at different stages of crop growth in northern plains. J Med Aro Pl Sci 22: 774-786.
  • Lawrence BM (1985) A review of the world production of essential oils (1984). Perfumer & Flavorist 10: 1-16.
  • Lawrence BM (1997) Progress in Essential Oils: Peppermint Oil. Perfumer & Flavorist 22: 57-66.
  • Linsmayer EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plantarum 18: 100-127.
  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Plant Physiol 21: 487-492.
  • Maffei M (1999) Sustainable methods for a sustainable production of peppermint (Menta × piperita L.) Essential Oil. J Essent Oil Res 11: 267-282.
  • Maffei M, Bertea CM, Mucciarelli M. (2007). Anatomy, physiology, biosynthesis, molecular biology, tissue culture, and biotechnology of mint essential oil production. In Mint: The genus Mentha, Lawrence BM ed, pp 41-85. CRC Press, Boca Raton, Florida.
  • Maffei M, Mucciarelli M, Scannerini S (1994) Are leaf area index (LAI) and flowering related to oil productivity in peppermint? Flavour Frag J 9: 119-124.
  • Marotti M, Piccaglia R, Giovanelli E, Deans SG, Eaglesham E (1994) Effects of planting time and mineral fertilization on peppermint (Mentha piperita L.) essential oil composition and its biological activity. Flavour Frag J 9: 125-129.
  • McConkey ME, Gershenzon J, Croteau RB (2000) Developmental regulation of monoterpene biosynthesis in the glandular trichomes of peppermint. Plant Physiol 122: 215-223.
  • Misra A, Srivastava NK (2000) Influence of water stress on Japanese mint. J Herbs Spices Med Plants 7: 51-58.
  • Murray MJ, Hefendehl FW (1972) Changes in monoterpene composition of Mentha aquatica produced by gene substitution from M. arvensis. Phytochemistry 11: 2469-2474.
  • Murray MJ, Marble P, Lincoln D, Hefendehl FW (1988) Peppermint oil quality differences and the reason for them. In Flavor and Fragrances: A World Perspective, Lawrence BM, Mookherjee BD, Willis BJ, eds, pp 189-210. Elsevier Press, Amsterdam.
  • Rohloff J (1999) Monoterpene composition of essential oil from peppermint (Mentha x piperita L.) with regard to leaf position using solid-phase microextraction and gas chromatography/mass spectrometry analysis. J Agric Food Chem 47: 3782-3786.
  • Shah SC, Gupta LK (1989) Response of mentha species to different harvesting intervals. Prog Hort 21: 148-150.
  • Sharma S, Tyagi BR (1991) Character correlation, path coefficient and heritability analyses of essential oil and quality components in corn mint. J Genet 45: 257-262.
  • Spencer A, Hamill JD, Rhodes MJC (1993) In vitro biosynthesis of monoterpenes by Agrobacterium transformed shoots cultures of two Menta species. Phytochemistry 32: 911-919.
  • Srivastava NK, Misra A, Sharma S (2003) Variation among commercial cultivars of Japanese mint (Mentha arvensis L.) in the morphological and metabolite characters associated with essential oil yield. J Hortic Sci Biot Technol 78: 154-160.
  • Stojanova A, Paraskevova P, Anastassov Ch, (2000) A comparative investigation on the essential oil composition of two Bulgarian cultivar of Mentha piperita L. J Essent Oil Res 12: 438-440.
  • Thomassen D, Slattery JT, Nelson SD (1990) Menthofuran-dependent and independent aspects of pulegone hepatotoxicity: roles of glutathione. J Pharmacol Exp Ther 253: 567-572.
  • Tisserat B, Silman R (2000) Interactions of culture vessels, media volume, culture density, and carbon dioxide levels on lettuce and spearmint shoot growth in vitro. Plant Cell Rep 19: 464-471.
  • Tucker AO, De Baggio T (2000) In The big book of herbs, pp 688. Interweave Press, Loveland, CO.
  • Tucker AO, Naczi RFC (2007) Mentha: an overview of its classification and relationships. In Mint. The Genus Mentha. Medicinal and Aromatic Plants - Industrial Profiles (1-39). Lawrence BM red. CRC Press, Boca Raton, Florida, Taylor & Francis Group.
  • Turner GW, Croteau R (2004) Organization of monoterpene biosynthesis in mentha. immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase. Plant Physiol 136: 4215-4227.
  • Voirin B, Brun N, Bayet C (1990) Effects of daylength on the monoterpene composition of leaves of Mentha × piperita. Phytochemistry 29: 749-755.
  • Weglarz Z, Zalecki R (1985) Investigations of dependence of the crop season of peppermint (Mentha piperita L.) herb upon the crop itself and the quality of the raw material. Herba Pol 31: 175-180.
  • Weglarz Z, Zalecki R (1987). Effect of plantation age, herb harvest time and time of rhizome digging on the value of rhizome seedlings (Mentha piperita L.). Herba Pol 33: 43-48.
  • Wise ML, Croteau R (1999) Monoterpene biosynthesis. In Comprehensive natural products chemistry: isoprenoids. Cane ed, pp 9715. Elsevier, Oxford.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p417kz
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