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2012 | 59 | 3 | 395-400
Article title

Regulatory effects of 1,25-dihydroxyvitamin D3 on vascular smooth muscle cells

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EN
Abstracts
EN
Inflammatory response has been recognized as a central feature in the development and progression of atherosclerosis, and VSMCs (Vascular Smooth Muscle Cells) - the main cellular component of media, play an important role in this process. Many reports indicate that the biologically active vitamin D metabolite - 1,25-dihydroxyvitamin D3 (1,25(OH)2D3 = calcitriol), besides its well established role in calcium homeostasis, plays an essential role in the regulation of the inflammation process. The aim of this study was to determine the regulatory effects of calcitriol, applied at two supra-physiological doses (10 nM and 100 nM), in VSMC culture. Secretion of the pro-inflammatory cytokines, IL-6 and TNF-α, was significantly attenuated in calcitriol-treated VSMC culture, but the level of anti-inflammatory TGF-β was generally unchanged. Since in advanced atherosclerosis lesions several cell types, including VSMCs, overproduce the HSP70 chaperone protein, we also checked the effects of calcitriol on its synthesis. The presence of 1,25(OH)2D3 did not affect HSP70 synthesis under physiological conditions but the synthesis of HSP70 in VSMCs exposed to heat shock was significantly inhibited by calcitriol (=100 nM). We observed that 1,25(OH)2D3 induced SOD 1 activity, stimulated the expression of IκB-α, and did not influence the level of NF-κB-p65 in VSMCs. The results of our study suggest that 1,25(OH)2D3 may serve as a natural anti-inflammatory agent and may therefore play a beneficial role in the physiology of VSMC in some contexts of atherosclerosis.
Keywords
EN
Publisher

Year
Volume
59
Issue
3
Pages
395-400
Physical description
Dates
published
2012
received
2012-04-01
revised
2012-07-24
accepted
2012-08-19
(unknown)
2012-08-21
Contributors
author
  • Department of Plant Physiology and Biotechnology, University of Gdańsk, Gdańsk, Poland
  • Department of Clinical Immunology & Transplantology, Medical University of Gdańsk, Gdańsk, Poland
author
  • Department of Electron Microscopy, Medical University of Gdańsk, Gdańsk, Poland
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p395kz
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