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2012 | 59 | 3 | 391-393
Article title

An improved method for RNA isolation from plants using commercial extraction kits

Content
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EN
Abstracts
EN
Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
Keywords
Publisher

Year
Volume
59
Issue
3
Pages
391-393
Physical description
Dates
published
2012
received
2012-03-15
revised
2012-05-28
accepted
2012-07-16
(unknown)
2012-07-24
Contributors
  • Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland
  • Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland
  • Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland
  • Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p391kz
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