An improved method for RNA isolation from plants using commercial extraction kits

• Authors: Elżbieta Kalinowska , Maria Chodorska , Elżbieta Paduch-Cichal , Karolina Mroczkowska
• Languages of publication: EN

Abstracts

EN

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.

Keywords

EN

total RNA; secondary metabolites

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 3
• Pages: 391-393

Dates

published

2012

received

2012-03-15

revised

2012-05-28

accepted

2012-07-16

(unknown)

2012-07-24

Contributors

author

Elżbieta Kalinowska

• Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland

author

Maria Chodorska

• Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland

author

Elżbieta Paduch-Cichal

• Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland

author

Karolina Mroczkowska

• Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw, Poland

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