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2012 | 59 | 3 | 371-375
Article title

PDE4 inhibitors have no effect on eotaxin expression in human primary bronchial epithelial cells

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Abstracts
EN
The bronchial epithelium is a very important factor during the inflammatory response, it produces many key regulators involved in the pathophysiology of asthma and COPD. Local influx of eosinophils, basophils, Th2 lymphocytes and macrophages is the source of many cytotoxic proteins, cytokines and other mediators of inflammation. These cells are attracted by eotaxins (eotaxin-1/CCL11, eotaxin-2/CCL24, eotaxin-3/CCL26). Inhibitors of phosphodiesterase 4 (PDE4) are new anti-inflammatory drugs which cause cAMP accumulation in the cell and inhibit numerous stages of allergic inflammation. The aim of our study was to evaluate the influence of PDE4 inhibitors: rolipram and RO-20-1724 on the expression of eotaxins in human primary bronchial epithelial cells. Cells were preincubated with PDE4 inhibitors for 1 hour and then stimulated with IL-4 or IL-13 alone or in combination with TNF-α. After 48 hours, eotaxin protein level was measured by ELISA and mRNA level by real time PCR. These cells produce CCL24 and CCL26. PDE4 inhibitors increased CCL24 and CCL26 mRNA level irrespectively of the used stimulators. Rolipram and RO-20-1724 had no effect on eotaxin protein production in our experimental conditions. Thus PDE4 inhibitors have no effect on eotaxin protein expression in human primary bronchial epithelial cells. In vitro experiments should be performed using a primary cell model rather than immortalized lines.
Publisher

Year
Volume
59
Issue
3
Pages
371-375
Physical description
Dates
published
2012
received
2012-03-26
revised
2012-07-27
accepted
2012-08-24
(unknown)
2012-09-03
Contributors
  • Department of Internal Medicine, Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland
  • Department of Internal Medicine, Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland
  • Department of Internal Medicine, Pneumonology and Allergology, Medical University of Warsaw, Warsaw, Poland
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Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p371kz
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