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2012 | 59 | 3 | 353-356
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Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol

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Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
Physical description
  • A&A Biotechnology, Gdynia, Poland
  • A&A Biotechnology, Gdynia, Poland
  • A&A Biotechnology, Gdynia, Poland
  • A&A Biotechnology, Gdynia, Poland
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
  • Biebl H (2001) Fermentation of glycerol by Clostridium pasteurianum - batch and continuous culture studies. J Ind Microbiol Biotechnol 27: 18-26.
  • Boenigk R, Bowien S, Gottschalk G (1993) Fermentation of glycerol to 1,3-propanediol in continuous cultures of Citrobacter freundii. Appl Microbiol Biotechnol 38: 453-457.
  • Burkhard O, Grunwaldt E, Mahmoud O, Jennewein S (2009) Genome shuffling in Clostridium diolis DSM 15410 for improved 1,3-propanediol production. Appl Environ Microbiol 75: 7610-7616.
  • Chatzifragkou A, Papanikolaou S, Dietz D, Doulgeraki AI, Nychas GJ, Zeng AF (2011) Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process. Appl Microbiol Biotechnol 91: 101-112.
  • Dabrock B, Bahl H, Gottschalk G (1992) Parameters affecting solvent production by Clostridium pasteurianum. Appl Environ Microbiol 58: 1233-1239.
  • Dąbrowski S, Pietrewicz-Kubicz D, Zabłotna E, Długołęcka A (2012) 1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum. Acta Biochim Pol 59: 353-356.
  • Dohrmann AB, Baumert S, Klingebiel L, Weiland P, Tebbe CC (2011) Bacterial community structure in experimental methanogenic bioreactors and search for pathogenic clostridia as community members. Appl Microbiol Biotechnol 89: 1991-2004.
  • Emptage M, Haynie SL, Laffend LA, Pucci JP, Whited G (2009) Process for the biological production of 1,3-propanediol with high titter. Pub: US 2009/0253192.
  • Eliot AC, Gatenby AA, Van Dyk TK (2011) Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose. Pub: US 2011/0136190.
  • GlobalData (2010) Global Biodiesel Market Analysis and Forecasts to 2020.
  • Gungormusler M, Gonen C, Ozdemir G, Azbar N (2010) 1,3-Propanediol production potential of Clostridium saccharobutylicum NRRL B-643. N Biotechnol 27: 782-788.
  • Gupta A, Murarka A, Campbell P, Gonzalez R (2009) Anaerobic fermentation of glycerol in Paenibacillus macerans: methabolic pathway and environmental determinants. Appl Environ Microbiol 75: 5871-5883.
  • Kolesárová N, Hutňan M, Bodík I, Spalková V (2011) Utilization of biodiesel by-products for biogas production. J Biomed Biotechnol 2011: 126798.
  • Liu H, Xu Y, Zheng Z, Liu D (2010) 1,3-Propanediol and its copolymers: research, development and industrialization. Biotechnol J 5: 1137-1148.
  • Mu Y, Teng H, Zhang DJ, Wang W, Xiu ZL (2006) Microbial production of 1,3-propanediol by Klebsiella pneumoniae using crude glycerol from biodiesel preparations. Biotechnol Lett 28: 1755-1759.
  • Pasteris SE, Strasser de Saad AM (2009) Sugar-glycerol cofermentations by Lactobacillus hilgardii isolated from wine. J Agric Food Chem 57: 3853-3858.
  • Saitou M, Nei M (1987) The neighbor-joining method: a new method for reconstructing Guide Trees. Mol Biol Evol 4: 406-425.
  • Taconi KA, Venkataramanan KP, Johnson DT (2009) Growth and solvent production by Clostridium pasteurianum ATCC®6013TM utilizing biodiesel-derived crude glycerol as the sole carbon source. Environ Prog Sust Energy 28: 100-110.
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697-703.
  • Yazdani SS, Gonzalez R (2007) Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol 18: 213-219.
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