Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens

• Authors: Renata Koníčková , Alena Jirásková , Jaroslav Zelenka , Ladislav Lešetický , Martin Štícha , Libor Vítek
• Languages of publication: EN

Abstracts

EN

Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.

Keywords

EN

bilirubin ditaurate; bile pigments; urobilinoids; intestinal metabolism; Clostridium perfringens

• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 2
• Pages: 289-292

Dates

published

2012

received

2011-11-19

revised

2012-01-17

accepted

2012-04-26

(unknown)

2012-04-27

Contributors

author

Renata Koníčková

• Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

author

Alena Jirásková

• Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

author

Jaroslav Zelenka

• Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

author

Ladislav Lešetický

• Department of Organic and Nuclear Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic

author

Martin Štícha

• Department of Organic and Nuclear Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic

author

Libor Vítek

• Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

References

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