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2012 | 59 | 2 | 275-278
Article title

High efficiency method to obtain supercoiled DNA with a commercial plasmid purification kit

Content
Title variants
Languages of publication
EN
Abstracts
EN
Supercoiled state corresponds to the active form for plasmid applications. The relaxed circular form of plasmids is often inactive or poorly active. To obtain significant amounts of almost fully supercoiled DNA, we modified the standard protocol of a commercially available Qiagen plasmid purification kit. Our changes led to isolation of almost 100% of the plasmids in the supercoiled state. The modified protocol was used to purify different plasmids with consistent results. The purified plasmids maintain supercoiled state for about two months. The modified protocol is very advantageous because it allows easy DNA production with high degree of supercoiled form at low cost.
Year
Volume
59
Issue
2
Pages
275-278
Physical description
Dates
published
2012
received
2011-11-19
revised
2012-05-11
accepted
2012-06-01
(unknown)
2012-06-11
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p275kz
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