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2012 | 59 | 2 | 261-264
Article title

Effect of substrate stiffness on differentiation of umbilical cord stem cells

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Tissue formation and maintenance is regulated by various factors, including biological, physiological and physical signals transmitted between cells as well as originating from cell-substrate interactions. In our study, the osteogenic potential of mesenchymal stromal/stem cells isolated from umbilical cord Wharton's jelly (UC-MSCs) was investigated in relation to the substrate rigidity on polyacrylamide hydrogel (PAAM). Osteogenic differentiation of UC-MSCs was enhanced on stiff substrate compared to soft substrates, illustrating that the mechanical environment can play a role in differentiation of this type of cells. These results show that substrate stiffness can regulate UC-MSCs differentiation, and hence may have significant implications for design of biomaterials with appropriate mechanical properties for regenerative medicine.
Physical description
  • Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland
  • Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland
  • Institute of Physics, Polish Academy of Sciences, Warszawa, Poland
  • Department of Regenerative Medicine, WIHE Institute of Hygiene and Epidemiology
  • Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland
  • Department of Biophysics and Human Physiology, Centre of Biostructure Research, Medical University of Warsaw, Warszawa, Poland
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