PL EN


Preferences help
enabled [disable] Abstract
Number of results
2012 | 59 | 2 | 255-260
Article title

Triterpenoid α-amyrin stimulates proliferation of human keratinocytes but does not protect them against UVB damage

Content
Title variants
Languages of publication
EN
Abstracts
EN
Rhaponticum carthamoides plants ("maral root") are widely used in Siberian folk medicine. The present study reports for the first time the presence of pentacyclic terpenoid, α-amyrin, in methanol extract from leaves of this plant. α-Amyrin induced proliferation of human keratinocytes (HaCaT) by about 18% while other extract components were ineffective. A panel of biochemical and cell-based assays testing the antioxidative and cytoprotective activites of α-amyrin indicated no antioxidative activity of this compound. α-Amyrin did not protect HaCaT cells against the damage caused by UVB radiation.
Publisher

Year
Volume
59
Issue
2
Pages
255-260
Physical description
Dates
published
2012
received
2011-10-17
revised
2012-03-01
accepted
2012-04-04
(unknown)
2012-05-11
Contributors
author
  • Department of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark
  • Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Gdansk, Poland
  • Department of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland
  • Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Gdansk, Poland
  • Department of Dermatology, Bispebjerg Hospital, Copenhagen, Denmark
References
  • Agyare C, Lechtenberg M, Deters A, Petereit F, Hensel A (2011) Ellagitannins from Phyllanthus muellerianus (Kuntze) Exell.: Geraniin and furosin stimulate cellular activity, differentiation and collagen synthesis of human skin keratinocytes and dermal fibroblasts. Phytomedicine 18: 617-624.
  • Akihisa T, Kojima N, Kikuchi T, Yasukawa K, Tokuda H, T Masters E, Manosroi A, Manosroi J (2010) Anti-inflammatory and chemopreventive effects of triterpene cinnamates and acetates from shea fat. J Oleo Sci 59: 273-80.
  • Allouche Y, Warleta F, Campos M, Sánchez-Quesada C, Uceda M, Beltrán G, Gaforio JJ (2011) Antioxidant, antiproliferative, and pro-apoptotic capacities of pentacyclic triterpenes found in the skin of olives on MCF-7 human breast cancer cells and their effects on DNA damage. J Agric Food Chem 59: 121-130.
  • Anaya AL, Mata R, Sims JJ, González-Coloma A, Cruz-Ortega R, Guadaño A, Hernández-Bautista BE, Midland SL, Ríos R, Gómez-Pompa A (2003) Allelochemical potential of Callicarpa acuminata. J Chem Ecol 29: 2761-2776.
  • Aslam MN, Lansky EP, Varani J (2006) Pomegranate as a cosmeceutical source: pomegranate fractions promote proliferation and procollagen synthesis and inhibit matrix metalloproteinase-1 production in human skin cells. J Ethnopharmacol 103: 311-318.
  • Babior BM (1984) The respiratory burst of phagocytes. J Clin Invest 73: 599-601.
  • Barros FW, Bandeira PN, Lima DJ, Meira AS, de Farias SS, Albuquerque MR, Santos HS, Lemos TL, de Morais MO, Costa-Lotufo LV, Pessoa Cdo Ó (2011) Amyrin esters induce cell death by apoptosis in HL-60 leukemia cells. Bioorg Med Chem 19: 1268-1276.
  • Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': the FRAP assay. Anal Biochem 239: 70-76.
  • Biskup E, Szynklarz B, Kumirska J, Golebiowski M, Stepnowski P and Lojkowska E (2007) Comparison of biological activity and composition of extracts from Rhaponticum carhamoides grown on Polish and Siberian plantations. Planta Med 73: 909.
  • Biskup E, Lojkowska E (2009) Evaluation of biological activities of Rhaponticum carthamoides extracts. J Med Plant Res 3: 1092-1098.
  • Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 106: 761-771.
  • Brunold C, Deters A, Knoepfel-Sidler F, Hafner J, Müller B, Hensel A (2004) Polysaccharides from Hibiscus sabdariffa flowers stimulate proliferation and differentiation of human keratinocytes. Planta Med 70: 370-373.
  • Cai BX, Luo D, Lin XF, Gao J (2008) Compound K suppresses ultraviolet radiation-induced apoptosis by inducing DNA repair in human keratinocytes. Arch Pharm Res 31: 1483-1488.
  • Chaturvedula VS, Schilling JK, Miller JS, Andriantsiferana R, Rasamison VE, Kingston DG (2004) New cytotoxic terpenoids from the wood of Vepris punctata from the Madagascar Rainforest. J Nat Prod 67: 895-898.
  • Chobot V, Buchta V, Jahodárová H, Pour M, Opletal L, Jahodár L, Harant P (2003) Antifungal activity of a thiophene polyine from Leuzea carthamoides. Fitoterapia 74: 288-290.
  • Chung IM, Kim MY, Park SD, Park WH, Moon HI (2009) In vitro evaluation of the antiplasmodial activity of Dendropanax morbifera against chloroquine-sensitive strains of Plasmodium falciparum. Phytother Res 23: 1634-1637.
  • Dinkova-Kostova AT, Jenkins SN, Wehage SL, Huso DL, Benedict AL, Stephenson KK, Fahey JW, Liu H, Liby KT, Honda T, Gribble GW, Sporn MB, Talalay P (2008) A dicyanotriterpenoid induces cytoprotective enzymes and reduces multiplicity of skin tumors in UV-irradiated mice. Biochem Biophys Res Commun 367: 859-865.
  • Faurschou A, Gniadecki R, Calay D, Wulf HC (2008) TNF-alpha impairs the S-G2/M cell cycle checkpoint and cyclobutane pyrimidine dimer repair in premalignant skin cells: role of the PI3K-Akt pathway. J Invest Dermatol 128: 2069-2077.
  • Holanda Pinto SA, Pinto LM, Guedes MA, Cunha GM, Chaves MH, Santos FA, Rao VS (2008) Antinoceptive effect of triterpenoid alpha,beta-amyrin in rats on orofacial pain induced by formalin and capsaicin. Phytomedicine 15: 630-634.
  • Jodynis-Liebert J, Murias M, Błoszyk E (2000) Effect of sesquiterpene lactones on antioxidant enzymes and some drug-metabolizing enzymes in rat liver and kidney. Planta Med 66: 199-205.
  • Kehrer JP (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol 23: 21-48.
  • Koleckar V, Brojerova E, Rehakova Z, Kubikova K, Cervenka F, Kuca K, Jun D, Hronek M, Opletalova V, Opletal L (2010) In vitro antiplatelet activity of flavonoids from Leuzea carthamoides. Drug Chem Toxicol 31: 27-35.
  • Konczak-Islam I, Yoshimoto M, Hou DX, Terahara N, Yamakawa O (2003) Potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced tissue of sweetpotato (Ipomoea batatas L.). J Agric Food Chem 51: 5916-5922.
  • Lee J, Jung E, Lee J, Huh S, Kim J, Park M, So J, Ham Y, Jung K, Hyun CG, Kim YS, Park D (2007) Panax ginseng induces human Type I collagen synthesis through activation of Smad signaling. J Ethnopharmacol 109: 29-34.
  • Leeuwenburgh C, Hansen P, Shaish A, Holloszy JO, Heinecke JW (1998) Markers of protein oxidation by hydroxyl radical and reactive nitrogen species in tissues of aging rats. Am J Physiol 274: R453-R461.
  • Lu Y, Foo Y (2001) Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 75: 197-202.
  • Melo CM, Carvalho KM, Neves JC, Morais TC, Rao VS, Santos FA, Brito GA, Chaves MH (2010) Alpha,beta-amyrin, a natural triterpenoid ameliorates L-arginine-induced acute pancreatitis in rats. World J Gastroenterol 16: 4272-4280.
  • Miliauskas G, van Beek TA, de Waard P, Venskutonis RP, Sudhölter EJ (2005) Identification of radical scavenging compounds in Rhaponticum carthamoides by means of LC-DAD-SPE-NMR. J Nat Prod 68: 168-172.
  • Müller K, Gürster D (1993) Hydroxyl radical damage to DNA sugar and model membranes induced by anthralin (dithranol). Biochem Pharmacol 46: 1695-1704.
  • Nikiéma JB, Vanhaelen-Fastré R, Vanhaelen M, Fontaine J, De Graef C, Heenen M (2001) Effects of antiinflammatory triterpenes isolated from Leptadenia hastata latex on keratinocyte proliferation. Phytother Res 15: 131-134.
  • Oliveira FA, Chaves MH, Almeida FR, Lima RC Jr, Silva RM, Maia JL, Brito GA, Santos FA, Rao VS (2005) Protective effect of alpha- and beta-amyrin, a triterpene mixture from Protium heptaphyllum (Aubl.) March. trunk wood resin, against acetaminophen-induced liver injury in mice. J Ethnopharmacol 98: 103-108.
  • Pavlík M, Laudová V, Grüner K, Vokác K, Harmatha J (2002) High-performance liquid chromatographic analysis and separation of N-feruloylserotonin isomers. J Chromatogr B Analyt Technol Biomed Life Sci 770: 291-295.
  • Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269: 337-341.
  • Reuter J, Wölfle U, Weckesser S, Schempp C (2010) Which plant for which skin disease? Part 1: Atopic dermatitis, psoriasis, acne, condyloma and herpes simplex. J Dtsch Dermatol Ges 8: 788-796.
  • Sharaf M, Skiba A, Weglarz Z, El-Ansari MA (2001) Two flavonol 5-O-glycosides from the roots of Leuzea carthamoides. Fitoterapia 72: 940-942.
  • Thannickal VJ, Fanburg BL (2000) Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol 279: L1005-L1028.
  • Umemura K, Itoh T, Hamada N, Fujita Y, Akao Y, Nozawa Y, Matsuura N, Iinuma M, Ito M (2008) Preconditioning by sesquiterpene lactone enhances H2O2-induced Nrf2/ARE activation. Biochem Biophys Res Commun 368: 948-954.
  • Villaseñor IM, Canlas AP, Faustino KM, Plana KG (2004) Evaluation of the bioactivity of triterpene mixture isolated from Carmona retusa (Vahl.) Masam leaves. J Ethnopharmacol 92: 53-56.
  • Yance D (2004) Rhaponticum carthamoides: Monograph and Review of the Literature. American Herbalists Guide 5: 9-17.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p255kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.