PL EN


Preferences help
enabled [disable] Abstract
Number of results
2012 | 59 | 1 | 151-153
Article title

Agro-food wastes utilization by Blakeslea trispora for carotenoids production

Content
Title variants
Languages of publication
EN
Abstracts
EN
The all-trans-β-carotene is a natural pigment used in various industrial fields (food, cosmetics, pharmaceuticals, etc) and possesses the higher provitamin A activity, in respect to other carotenoids. All-trans-β-carotene is produced industrially by chemical and biotechnological means. For β-carotene biotechnological production in industrial scale mated cultures of Blakeslea trispora, a heterothallic fungus, are mainly used. Despite the intense research for β-carotene production by B. trispora, natural substrate utilization has not been extensively studied. Solid agro-food wastes such as cabbage, watermelon husk and peach peels from northern Greece as main carbon source into submerged B. trispora cultures for carotenoids production, was examined. The media containing only the agro-food waste (2-4) gave a biomass accumulation 7.77±0.4 g/L, while a reference medium 1 with glucose (10 g/L) gave 4.65±0.21 g/L. In another experiments series agro-food wastes were used with corn steep liquor and thiamine (media 6-8), giving a biomass accumulation and total carotenoid volumetric production 10.2±2.41 g/L and 230.49±22.97 mg/L, respectively. These are the higher values reported for solid wastes so far in respect to those obtained from a synthetic medium, with higher glucose concentration of 50 g/L where the correspondent values were 9.41±1.18 g/L and 45.63 mg/L respectively. The results support that B. trispora is able to utilize, almost equivalently, different origin agro-food wastes for carotenoids production. Furthermore, β-carotene percentage in all examined cases was over 76%, as it was estimated by HPLC analysis, suggesting that these agro food wastes may be used for high purity, large scale β carotene production.
Publisher

Year
Volume
59
Issue
1
Pages
151-153
Physical description
Dates
published
2012
received
2011-10-17
accepted
2012-03-01
(unknown)
2012-03-17
Contributors
  • Section of Chemistry, Faculty of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki, Greece
  • Laboratory of Natural Resources and Renewable Energies, Chemical Process Engineering Research Institute (CPERI), Centre for Research and Technology-Hellas (CERTH), Thessaloniki, Greece
References
  • Bendich A, Olson JA (1989) Biological actions of carotenoids. FASEB J 3: 1927-1932.
  • Echaverri-Erasum C, Johnson EA (2002) Fungal carotenoids. In: Applied mycology and biotechnology. Khachatourians GG, Arora DK eds, pp 45-87. Elsevier, Amsterdam.
  • Halliwell B (1997) Antioxidants and human disease: A general introduction. Nutr Rev 55: S44-S52.
  • Özdemir S, Güven K, Baysal Z, Uyar F (2009) Screening of various organic substrates and the development of a suitable low-cost fermentation medium for the production of a-amylase by Bacillus subtilis. Food Technol Biotechnol 47: 364-369.
  • Palozza P, Krinsky NI (1992) Antioxidant effects of carotenoids in vivo and in vitro: an overview. Meth Enzymol 213: 403-420.
  • Papaioannou E, Roukas T, Liakopoulou-Kyriakides M (2008) Effect of biomass pre-treatment and solvent extraction on β-carotene and lycopene recovery from Blakeslea trispora cells. Prep Biochem Biotechnol 38: 246-256.
  • Papaioannou EH, Liakopoulou-Kyriakides M (2010) Substrate contribution on carotenoids production in Blakeslea trispora cultivations. Food Bioprod Proces 88: 305-311.
  • Rosales E, Rodríguez Couto S, Sanromán A (2002) New uses of food waste: application to laccase production by Trametes hirsuta. Biotechnol Lett 24: 701-704.
  • Stabnikova O, Wang JY, Ding HB, Tay JH (2005) Biotransformation of vegetable and fruit processing wastes into yeast biomass enriched with selenium. Bioresour Technol 96: 747-751.
  • Valduga E, Valério A, Treichel H, Júnior AF, Di Luccio M (2009) Kinetic and stoichiometric parameters in the production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) in synthetic and agroindustrial media. Appl Biochem Biotechnol 157: 61-69.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p151kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.