Atomic force microscopy studies of the adhesive properties of DPPC vesicles containing β-carotene

• Authors: Dominika Augustyńska , Małgorzata Jemioła-Rzemińska , Kvetoslava Burda , Kazimierz Strzałka
• Languages of publication: EN

Abstracts

EN

A role of carotenoids as modulators of physical properties of model and biological membranes has been already postulated. However, there is a lack of information on the influence of these pigments on interactions between the lipids which form such membranes. This paper applies atomic force microscopy (AFM) in to study the effects of β-carotene on the adhesion properties of DPPC multilamellar liposomes. This allowed us to gain, for the first time, a direct insight into the interactions between the components in model systems on a molecular level. We observe that the adhesive forces in DPPC multilamellar liposomes containing 1mol% of β-carotene decrease exponentially with increasing temperature, and that at about 37°C they diminish. In the case of pure liposomes the decline in adhesion is of a different nature and the adhesive forces disappear at 34°C. The adhesive forces are about 5 times higher at 31°C in the presence of β-carotene than in its absence. However, measurements using differential scanning calorimetry (DSC) showed a shift of the lamellar-to-undulled-lamellar phase transition toward lower temperatures by about 0.8±0.2°C in a system containing β-carotene. The enthalpy changes (ΔH) of this transition are similar for both systems. For the main transition, gel-to-liquid crystalline, the peak is shifted by about 0.5±0.1°C, and ΔH decreases by about 30% in liposomes treated with β-carotene in comparison to pure liposomes. Our results suggest increased cooperation between liposome components in a system with enriched β-carotene, which cause a change in phase transition temperatures. Moreover, these interactions are very sensitive to temperature.

Keywords

EN

atomic force microscopy; dipalmitoylphosphatidylcholine; β-carotene; liposome adhesion; thermal transition

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2012
• Volume: 59
• Issue: 1
• Pages: 125-128

Dates

published

2012

received

2011-11-19

accepted

2012-03-01

(unknown)

2012-03-17

Contributors

author

Dominika Augustyńska

• AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Kraków, Poland

author

Małgorzata Jemioła-Rzemińska

• Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Kraków, Poland

author

Kvetoslava Burda

• AGH University of Science and Technology, Faculty of Physics and Applied Computer Science, Kraków, Poland

author

Kazimierz Strzałka

• Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Kraków, Poland

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