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2012 | 59 | 1 | 125-128
Article title

Atomic force microscopy studies of the adhesive properties of DPPC vesicles containing β-carotene

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A role of carotenoids as modulators of physical properties of model and biological membranes has been already postulated. However, there is a lack of information on the influence of these pigments on interactions between the lipids which form such membranes. This paper applies atomic force microscopy (AFM) in to study the effects of β-carotene on the adhesion properties of DPPC multilamellar liposomes. This allowed us to gain, for the first time, a direct insight into the interactions between the components in model systems on a molecular level. We observe that the adhesive forces in DPPC multilamellar liposomes containing 1mol% of β-carotene decrease exponentially with increasing temperature, and that at about 37°C they diminish. In the case of pure liposomes the decline in adhesion is of a different nature and the adhesive forces disappear at 34°C. The adhesive forces are about 5 times higher at 31°C in the presence of β-carotene than in its absence. However, measurements using differential scanning calorimetry (DSC) showed a shift of the lamellar-to-undulled-lamellar phase transition toward lower temperatures by about 0.8±0.2°C in a system containing β-carotene. The enthalpy changes (ΔH) of this transition are similar for both systems. For the main transition, gel-to-liquid crystalline, the peak is shifted by about 0.5±0.1°C, and ΔH decreases by about 30% in liposomes treated with β-carotene in comparison to pure liposomes. Our results suggest increased cooperation between liposome components in a system with enriched β-carotene, which cause a change in phase transition temperatures. Moreover, these interactions are very sensitive to temperature.
Physical description
  • Andreeva A, Popova A (2010) Integration of β-carotene molecules in small liposomes. J Phys: Conf Ser 253: 012066.
  • Bakowsky H, Richter T, Kneuer C, Hoekstra D, Rothe U, Bendas G, Ehrhardt C, Bakowsky U (2008) Adhesion characteristics and stability assessment of lectin-modified liposomes for site-specific drug delivery. Biochim Biophys Acta 1778: 242-249.
  • Bendich A (1993) Biological functions of dietary carotenoids. Ann NY Acad Sci 691: 61-67.
  • Biltonen RL and Lichtenberg D (1993) The use of differential scanning calorimetry as a tool to characterize liposome preparations. Chem Phys Lipids 64: 129-142.
  • Britton G (2008) Functions of intact carotenoids. In Carotenoids. Natural Functions. Britton G, Liaaen-Jensen S, Pfander, eds, vol 4, pp 189-211. Birkhauser Verlag.
  • Burton GW, Ingold KU (1984) Beta-carotene: an unusual type of lipid antioxidant. Science 224: 569-573.
  • Cappella B, Dietler G (1999) Force-distance curves by atomic force microscopy. Surf Sci Rep 34: 1-104
  • De Souza EF, Teschke O (2003) Liposome stability verification by atomic force microscopy. Rev Adv Mater Sci 5: 34-40.
  • Duzgunes N, Nir S (1999) Mechanisms and kinetics of liposome-cell interactions. Adv Drug Deliv Rev 40: 3-18.
  • Eastman T, Zhu DM (1996) Adhesion forces between surface-modified AFM tips and a mica surface. Langmuir 12: 2859-2862.
  • Fujihira M, Aoki D, Okabe Y, Takano H, Hokari H, Frommer J, Nagatani Y, Sakai F (1996) Effect of capillary force on friction force microscopy: a scanning hydrophilicity microscope. Chem Lett 7: 499-500.
  • Gruszecki WI, Strzałka K (2005) Carotenoids as modulators of lipid membrane physical properties. Biochim Biophys Acta 1740: 108-115.
  • Iglič A (2011) Advances in planar lipid bilayers and liposomes. Andreeva A, Popova A, eds, vol 14, pp 294-307. Academic Press.
  • Jemioła-Rzemińska M, Pasenkiewicz-Gierula M, Strzałka K (2005) The behaviour of β-carotene in the phosphatidylcholine bilayer as revealed by a molecular simulation study. Chem Phys Lipids 135: 27-37.
  • Kostecka-Gugała A, Latowski D, Strzałka K (2003) Thermotropic phase behaviour of α-dipalmitoylphosphatidylcholine multibilayers is influenced to various extents by carotenoids containing different structural features - evidence from differential scanning calorimetry. Biochim Biophys Acta 1609: 193-202.
  • Roiter Y, Ornatska M, Rammohan AR, Balakrishnan J, Heine DR, Minko S (2009) Interaction of lipid membrane with nanostructured surfaces. Langmuir 25: 6287-6299.
  • Sanchez J, Badia A (2003) Atomic force microscopy studies of lateral phase separation in mixed monolayers of dipalmitoylphosphatidylcholine and dilauroylphosphatidylcholine. Thin Solid Films 440: 223-239.
  • Suwalsky M, Hidalgo P, Strzałka K, Kostecka-Gugała A (2002) Comparative X-ray studies on the interaction of carotenoids with a model phosphatidylcholine membrane. Z Naturforsch C 57: 129-134.
  • Telfer A, Pascal A, Gall A (2008) Carotenoids in Photosynthesis. In Carotenoids. Natural Functions. Britton G, Liaaen-Jensen S, Pfander H, eds, vol 4, pp 265-305. Birkhauser Verlag.
  • Yang YP, Tsay RY (2007) Morphology of compressed dipalmitoyl phosphatidylcholine monolayers investigated by atomic force microscopy. J Lumin 127: 186-191.
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