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2012 | 59 | 1 | 101-103
Article title

Violaxanthin and diadinoxanthin de-epoxidation in various model lipid systems

Content
Title variants
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EN
Abstracts
EN
The xanthophyll cycle is an important photoprotective process functioning in plants. One of its forms, the violaxanthin (Vx) cycle, involves interconversion between: Vx, antheraxanthin (Ax) and zeaxanthin (Zx). Another kind of the xanthophyll cycle is the diadinoxanthin (Ddx) cycle in which interconversion between Ddx and diatoxanthin (Dtx) occurs. In this study an information on molecular mechanism and regulation of these two types of the xanthophyll cycle is presented. The influence of lipids on the de-epoxidation of the xanthophyll cycle pigments was investigated, with special focus put on the significance of physical properties of the aggregates formed by inverted lipid micelles, which are necessary for activity of the xanthophyll cycle enzymes. In particular, thickness of the hydrophobic fraction of the aggregates, size of the inverted micelles, suggested by mathematical description of the structures and solubility of Vx and Ddx in various kind of lipids were studied. Obtained results show that the rate of de-epoxidation is strongly dependent on the physicochemical properties of the lipids used. The key role for enzyme activation play non-bilayer lipids and the parameters of inverted micelles such as thickness, fluidity of hydrophobic core and their diameter. The presented results show that MGDG and other non-lamellar lipids like different forms of phosphatidylethanolamine are necessary for the Vx and Ddx de-epoxidation because they provide the three-dimensional structures, which are needed for the binding of de-epoxidases and for the accessibility of Vx and Ddx to these enzymes.
Year
Volume
59
Issue
1
Pages
101-103
Physical description
Dates
published
2012
received
2011-10-17
accepted
2012-03-01
(unknown)
2012-03-17
References
  • Adamska I (1997) ELIPs-light-induced stress proteins. Review. Physiol Plant 100: 794-805.
  • Frommolt R, Goss R, Wilhelm C (2001) The de-epoxidase and epoxidase reactions of Mantoniella squamata (Prasinophyceae) exhibit different substrate-specific reaction kinetics compared to spinach. Planta 213: 446-456.
  • Goss R, Latowski D, Grzyb J, Vieler A, Lohr M, Wilhelm C, Strzałka K (2007) Lipid dependence of diadinoxanthin solubilization and de-epoxidation in artificial membrane systems resembling the lipid composition of the natural thylakoid membrane. Biochim Biophys Acta 1768: 67-75.
  • Goss R, Lohr M, Latowski D, Grzyb J, Vieler A, Wilhelm C, Strzałka K (2005) Role of hexagonal structure forming lipids in diadinoxanthin and violaxanthin solubilization and de-epoxidation. Biochemistry 44: 4028-4036.
  • Grouneva I, Jakob T, Wilhelm C, Goss R (2006) Influence of ascorbate and pH on the activity of the diatom xanthophyll cycle-enzyme diadinoxanthin de-epoxidase. Physiol Plant 126: 205-211.
  • Hager A (1969) Lichtbedingte pH-Erniedrigung in Einem Chloroplastenkompartiment als Ursache der Enzymatischen Violaxanthin-Zeaxanthin-Umwandlung; Beziehungen zur Photophosphorylierung. Planta 89: 224-243.
  • Hager A, Holocher K (1994) Localization of the xanthophyll-cycle enzyme violaxanthin de-epoxidase within the thylakoid lumen and abolition of its mobility by a (light-dependent) pH decrease. Planta 192: 581-589.
  • Jakob T, Goss R, Wilhelm C (2001) Unusual pH-dependence of diadinoxanthin de-epoxidase activation causes chlororespiratory induced accumulation of diatoxanthin in the diatom Phaeodactylum tricornutum. J Plant Physiol 158: 383-390.
  • Latowski D, Akerlund HE, Strzałka K (2004) Violaxanthin de-epoxidase, the xanthophyll cycle enzyme, requires lipid hexagonal structures for its activity. Biochemistry 43: 4417-4420.
  • Latowski D, Kruk J, Burda K, Skrzynecka-Jaskier M, Kostecka-Gugała A, Strzałka K (2002) Kinetics of violaxanthin de-epoxidation by violaxanthin de-epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers. Eur J Biochem 269: 4656-4665.
  • Latowski D, Kuczyńska P, Strzałka K (2011) Xanthophyll cycle - a mechanism protecting plants against oxidative stress. Redox Rep 16: 78-90.
  • Lohr M, Wilhelm C (2001) Xanthophyll synthesis in diatoms: quantification of putative intermediates and comparison of pigment conversion kinetics with rate constants derived from a model. Planta 212: 382-391.
  • Pfündel EE, Bilger W (1994) Regulation and possible function of the violaxanthin cycle. Photosynth Res 42: 89-109.
  • Provasoli L, McLaughlin JJA, Droop MR (1957) The development of artificial media for marine algae. Arch Mikrobiol 25: 392-428.
  • Sprague GS, Staehelin LA (1984) Effect of reconstitution method on the structural organization of isolated chloroplast membrane lipids. Biochim Biophys Acta 777: 306-322.
  • Stransky H, Hager A (1970) The carotenoid pattern and the occurrence of the light-induced xanthophyll cycle in various classes of algae. VI. Chemosystematic study. Arch Mikrobiol 73: 315-323.
  • Yamamoto HY (1979) Biochemistry of the violaxanthin cycle in higher-plants. Pure Appl Chem 51: 639-648.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv59p101kz
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