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2011 | 58 | 4 | 535-540
Article title

How the RNA isolation method can affect microRNA microarray results

Content
Title variants
Languages of publication
EN
Abstracts
EN
The quality of RNA is crucial in gene expression experiments. RNA degradation interferes in the measurement of gene expression, and in this context, microRNA quantification can lead to an incorrect estimation. In the present study, two different RNA isolation methods were used to perform microRNA microarray analysis on porcine brain tissue. One method is a phenol-guanidine isothiocyanate-based procedure that permits isolation of total RNA. The second method, miRVana™ microRNA isolation, is column based and recovers the small RNA fraction alone. We found that microarray analyses give different results that depend on the RNA fraction used, in particular because some microRNAs appear very sensitive to the RNA isolation method. We conclude that precautions need to be taken when comparing microarray studies based on RNA isolated with different methods.
Publisher

Year
Volume
58
Issue
4
Pages
535-540
Physical description
Dates
published
2011
received
2011-04-12
revised
2011-11-09
accepted
2011-12-03
(unknown)
2011-12-06
Contributors
  • Department of Basic Animal and Veterinary Sciences, Section of Genetics and Bioinformatics, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
  • Bioinformatics Centre, Department of Biology and Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark
author
  • LEO Pharma A/S, Ballerup, Denmark
  • Department of Basic Animal and Veterinary Sciences, Section of Genetics and Bioinformatics, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
  • Department of Basic Animal and Veterinary Sciences, Section of Genetics and Bioinformatics, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv58p535kz
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