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In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase

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Glutathione reductase (GR, E.C is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.
Physical description
  • Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey
  • Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Ankara, Turkey
  • Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey
  • Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey
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