PL EN


Preferences help
enabled [disable] Abstract
Number of results
2011 | 58 | 3 | 297-302
Article title

The effects of velvet antler polypeptides on the phenotype and related biological indicators of osteoarthritic rabbit chondrocytes

Content
Title variants
Languages of publication
EN
Abstracts
EN
Objective: To study the effects of velvet antler polypeptides (VAPs) on osteoarthritic chondrocytes (OCs) in rabbits. Methods: An osteoarthritic rabbit model was established according to Hulth's method. OCs were isolated and cultured for observation of the cell cycle. Cell proliferation was detected by MTT assay and the cell cycle was monitored by flow cytometry. The phenotype was determined by toluidine blue staining as well as immunohistochemical staining for collagen type II. The expression of MMP-1, MMP-3, MMP-13, TIMP-1, and collagen I and X mRNA by chondrocytes was assayed by RT-PCR. Results: The VAPs had no obvious proliferative effect on OCs and did not affect the cell cycle. However, they significantly reduced the proportion of early apoptotic cells in a dose-dependent manner. Further, VAPs inhibited the expression of collagen I and X mRNA and induced abnormal expression of MMP-1 and MMP-13 mRNA. VAPs had no significant effect on MMP-3 and TIMP-1 mRNA levels. The toluidine blue and collagen type II immunohistochemical staining intensities of VAP-treated chondrocytes were positively correlated with the concentration of VAPs used. Conclusion: VAPs had no significant effect on OC proliferation and the cell cycle, but did increase the glycosaminoglycan (GAG) and collagen type II expression levels in the extracellular matrix, and down-regulated collagen I and X mRNA expression. Treatment of cartilage cells with VAPs maintained their normal phenotype, inhibited matrix metalloproteinases (MMPs) secretion, kept the balance of cartilage matrix metabolism, and sustained an external environment where the cartilage cells could survive. Moreover, VAPs reduced the proportion of early apoptotic cells, suggesting that they may block the apoptotic pathway in OCs.
Publisher

Year
Volume
58
Issue
3
Pages
297-302
Physical description
Dates
published
2011
received
2010-11-11
revised
2011-05-12
accepted
2011-07-03
(unknown)
2011-07-11
Contributors
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
author
  • Clinical Laboratory of Tumor Hospital of Jilin Province, Jilin, P.R.China
author
  • Department of Pharmacology, Henan University of Science and Technology, Henan, P.R. China
author
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
author
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
author
  • Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Jilin, P.R. China
References
  • Aigner T, Hemme Lm, Neureiter D (2001) Apoptotic cell death is not a wide spread phenomenon in no rmalaging and osteoarthritis human articular knee cartilage.Arthritis Rheum 44: 1304-1312.
  • Duan Lx (2007) Studies on the chemical structrue of velvet antler polypeptides (cervus Nippon temminck) and its anti-hepatic fibrosis. D, Jilin University.
  • Gauci SJ, Golub SB, Tutolo L, Little CB, Sims NA, Lee ER, Mackie EJ, Fosang AJ (2008) Modulating chondrocyte hypertrophy in growth plate and osteoarthritic cartilage. J Musculoskelet Neuronal Interact 8: 308-310.
  • Guo Y, Zhou Q, Liu P, Wang Y, Fang J, Wang B (1998) The research of pilose antler polypeptides promoting osteoblast precursor cells and chondrocytes proliferation. J Chinese J Biochem Pharmaceutics 19: 74-75.
  • Guo Q, Tian D, Ao Y (2003) The collagen phenotype of normal and osteoarthritis cartilages.J Chinese J Sports Med 22: 204-209.
  • Kim HA, Blanco FJ (2007) Cell death and apoptosis in osteoarthritic cartilage. Curr Drug Targets 8: 333-345.
  • Kim HA, Lee YJ, Seong SC, Choe KW, Song YW (2000) Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 27: 455-462.
  • Kim Y-K, Kim K-S, Chung K-H, Kim J-G, Kim K-S, Lee Y-C, Chang Y-C, Kim C-H (2003) Inhibitory effects of deer antler aqua-acupuncture, the pilose antler of Cervus Korean Temminck var.mantchuricus Swinhoe, on type II collagen-induced arthritis in rats. Int Immunopharmacol 3: 1001-1010.
  • Kim K-H, K-S, Choi B-J, Chung K-H, Chang Y-C, Lee S-D, Park K-K, Kim H-M, Kim C-H (2005) Anti-bone resorption activity of deer antler aqua-acupunture, the pilose antler of cervus korean TEMMINCK var. mantchuricus Swinhoe (Nokyong) in adjuvant-induced arthritic rats. J Ethnopharmacol 96: 497-506.
  • Kim KW, Song KH, Lee JM, Kim KS, Kim SI, Moon SK, Kang BS, Kim DS, Chung KH, Chang YC, Kim CH (2008) Effects of TGFbeta1 and extracts from Cervus korean TEMMINCK var. mantchuricus Swinhoe on acute and chronic arthritis in rats.J Ethnopharmacol 118: 280-283.
  • Kraan PM, Blaney Davidson EN, Blom A, Berg WB (2009) TGF-beta signaling in chondrocyte terminal differentiation and osteoarthritis: modulation and integration of signaling pathways through receptor-Smads. Osteoarthritis Cartilage 17: 1539-1545.
  • Liu X, Li X, Zhou J (2005) Experimental study on replicating knee osteoarthritis by modified Hulth's modeling method. J Chinese J Int Trad West Med 25: 1104-1108.
  • Pelletier JP (1990) Imbalance between the mehanisms of activation and inhibition of metallo-proteases in the early lesions of experimental osteoarthristis. Arthritis Rheum 33: 364-367.
  • Qu M, Kang Y (2000) Study on biological characteristics of hypertrophic chondrocytes of damaged articular cartilage.J Chinese J Sports Med 19: 76-81.
  • Von Der Mark K, Kirsch T, Nerlich A, Kuss A, Weseloh G, Glückert K, Stöss H (1992) Type X collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy. Arthritis Rheum 35: 806-811.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv58p297kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.